CD117、PDGFRA蛋白单独及联合DOG1检测在胃肠道间质瘤中的诊断价值

目的:探讨组织中CD117、PDGFRA两种蛋白表达在胃肠道间质瘤(gastrointestinal stromal tumors,GIST)中的诊断价值及联合DOG1蛋白检测的意义。方法:回顾性对99例GIST和25例非GIST肿瘤标本进行CD117、PDGFRA和DOG1蛋白表达进行检测并进行相关性分析。结果:GIST组CD117、PDGFRA和DOG1蛋白表达率分别为93.94%(93/99)、53.54%(53/99)和90.91%(90/99),非GIST组分别为4.00%(1/25)、4.00%(1/25)和12.00%(3/25),组间比较均有统计学意义(P<0.05);GIST组性别、年龄、肿瘤直径、肿瘤部位、组织学类型和危险度分级等临床病理参数与CD117、PDGFRA和DOG1蛋白表达无统计学意义(P>0.05);CD117、PDGFRA、DOG1、CD117和DOG1联合、PDGFRA和DOG1联合及三者联合判断GIST的敏感性分别为0.989、0.981、0.968、0.960、0.933和0.961,特异性分别为0.800、0.343、0.710、0.840、0.947和0.955,ROC曲线下面积(AUC)分别为0.945、0.748、0.895、0.895、0.840和0.975。结论:GIST中CD117、PDGFRA及DOG1蛋白表达在胃肠道间质瘤中的优势人群有待进一步研究;CD117、PDGFRA蛋白单独及联合DOG1检测可提高对GIST诊断的准确度。     

胃肠道间质瘤64例免疫标记物应用分析

目的 探讨胃肠道间质瘤(gastrointestinal stromal tumor,GIST)的免疫组化标记物的表达特征,为其诊断及鉴别诊断提供依据.方法 收集85例确诊的胃肠道及腹、盆腔间叶源性肿瘤标本,其中GIST 64例.免疫组化法检测CD117、CD34、DOG1、NSE、S-100、SMA和vimentin的表达.结果 GIST组中CD117、CD34、DOG1、NSE阳性率分别为89.1%、76.6%、96.9%及87.5%;非GIST组中的阳性率分别为4.8%、33.3%、28.6%和50.0%.CD117阴性的GIST组中CD34、NSE、DOG1阳性率分别为28.6%、71.4%和85.7%.结论 GIST中DOG1表达的敏感性高于CD117,在CD117阴性的病例中DOG1优于其他标记物.DOG1与CD117及NSE等联合应用于免疫组化检测足以明确绝大多数GIST的诊断.     

CD117阴性的胃肠道间质瘤中DOG1的表达及意义

目的 探讨CD117阴性的胃肠道间质瘤(gastrointestinal stromal tumor,GIST)中DOG1的表达及意义.方法 通过免疫组化法检测58例CD117阴性的GIST中DOG1的表达.结果 DOG1的阳性表达率为93.1%(54/58).DOG1的表达与临床病理相关因素(性别、年龄、部位、肿瘤大小)无相关性(P>0.05).按危险程度分级分类:极低度8例,低度12例,中度21例,高度17例.DOG1的阳性率在不同危险程度间差异无显著性(P>0.05).结论 DOG1是比CD117更敏感、更特异的诊断GIST的免疫学指标,但不能作为判断其危险程度的指标.     

胃肠、泌尿、会阴部间质瘤临床病理及免疫组织化学分析

目的 探讨胃肠道间质瘤(GIST)与胃肠道外GIST型间质瘤的组织学起源与病理特征。方法 对46例胃肠道及13例泌尿道、会阴部原诊断平滑肌瘤、平滑肌肉瘤、许旺瘤的病例作回顾性研究,观察其病理特点,应用免疫组织化学方法观察4种抗体(CD117、CD34、平滑肌肌动蛋白、S—100)的表达,对发生于不同部位的间质瘤进行对比分析。结果 45例为GIST组,CD117阳性表达率为93．3％,CD34阳性率88．9％;12例为胃肠道外GIST型间质瘤组,CDll7阳性表达率为83．3％,CD34阳性率75．0％;2例(其中1例为胃肠道)平滑肌瘤组,CDll7和CD34均为阴性,平滑肌肌动蛋白瘤细胞呈弥漫性强阳性表达。结论 CDll7和CD34标记阳性是确诊间质瘤最具有诊断价值的依据。推测GIST和胃肠道外GIST型间质瘤均系起源于一种非定向分化的、原始间充质干细胞。     

胃肠道间质瘤124例DOG1、WISP-1表达与临床意义

目的 探讨DOG1和WISP-1在胃肠道间质瘤(gastrointestinal stromal tumors,GIST)中诊断及预后的意义.方法 收集川北医学院附属医院病理科124例有完整临床病理资料的GIST,应用免疫组化EnVision两步法检测了DOG1和WISP-1蛋白在124例GIST中的表达情况,并与非GIST进行对照研究;应用χ2检验及Spearmen检验对结果进行统计学分析.结果 124例GIST中DOG1的阳性表达率为96%(119/124),CD117的阳性变表达率为91.0%(111/124),两者在GIST中的表达水平差异无统计学意义(P>0.05);GIST与非GIST中DOG1的表达水平差异有统计学意义(P<0.001);本组病例中WISP-1阳性率为80.6%(100/124),其中极低风险性、低风险性、中风险性和高风险性的阳性表达率分别为44.4%(4/9)、66.7%(26/39)、86.9%(17/20)和95.0%(38/40),其阳性表达率与美国国立卫生研究院(NIH)风险分级呈正相关关系(P<0.001);GIST与非GIST 中WISP-1的表达水平差异无统计学意义(P>0.05).结论 DOG1是GIST中一个敏感又特异的标记物,与CD117联用能提高GIST的诊断准确率,临床上可将其作为鉴别消化道间叶源性肿瘤的一线抗体;WISP-1的表达可能和GIST恶性进程有关,有可能作为评估GIST生物学行为的指标.     

DOG-1在胃肠道间质瘤中的表达及其临床意义

目的 探讨DOG-1在胃肠道间质瘤(GIST)中的表达及其临床意义.方法 应用免疫组织化学EnVision法检测DOG-1在84例GIST患者肿瘤组织中的表达,并与CD117、CD34标记进行比较观察.结果 GIST患者手术切除肿瘤组织主要由数量不等的梭形细胞和上皮样细胞组成,两种细胞可按不同比例混合性或单一性组成肿瘤的实体.DOG-1、CD117和CD34在极低度及低度危险性GIST组织中阳性表达率分别为91.3%(42/46)、95.7%(44/46)和82.6%(38/46),在中度及高度危险性GIST组织中其阳性表达率分别为100%(38/38)、100%(38/38)和78.9%(30/38),对照组真性平滑肌瘤、神经鞘瘤、纤维瘤病及正常胃肠道黏膜组织中DOG-1、CD117、CD34均无表达,DOG-1标记GIST的敏感性及特异性与CD117相似,差异无统计学意义(P＞0.05),而标记中度及高度危险性GIST的敏感性与特异性明显高于CD34,其表达差异有统计学意义(P＜0.01).结论 DOG-1是一种新的诊断GIST比较特异的标记,尤其是对中度及高度危险性GIST比CD34有更高的敏感性和特异性.与CD117联合应用将有利于进一步提高GIST的诊断水平.

Abstract：

Objective To investigate the expression of DOG-1 in gastrointestinal stromal tumors (GIST)and its diagnostic application.Methods Immunohistochemical EnVision technique was used to assess the expression of DOG-1 in 84 cases of GIST in comparison with CD117 and CD34.Results All 84cases of GIST consisted of variable proportions of spindle and epithelioid tumor cells or iust one type of the tumor cell. The expression rates of DOG-1,CD117 and CD34 were 91.3%(42/46),95.7%(44/46)and 82.6%(38/46),in the group of very low and low risk GIST,and were 100%(38/38),100%(38/38)and 78.9%(30/38),respectively,in the group of moderate and hiish risk GIST. leiomyomas,schwannomas,fibromatosis and normal gastrointestinal mucoca did not express these markers.Moreover, the sensitivity and specificity of DOG-1 in the detection of GIST were similar to those of CD117.without statistical difference(P＞0.05)between the two markers.However,the sensitivity and specificity of DOG-1 detection of moderate and high risk GIsT were significandy higher than those of CD34(P＜0.01).Conclusions DOG-1 is a novel marker of gastrointestinal stromal tumors.It has the sensitivity and specificity hisher than CD34,especially in the detection of moderate and high risk GIST.Combined DOG-1and CD117 immunohistochemistry will likely improve the diagnostic accuracy of GIST.     

nestin在胃肠道间质瘤病理诊断中的价值

目的探讨nestin在胃肠道间质瘤（gastrointestinal stromal tumors，GISTs）辅助诊断中的价值。方法用免疫组化EnVision法检测96例CD117阳性、5例CD117阴性的GISTs，以及食管平滑肌瘤（10例）、消化道雪旺瘤（33例）、肠纤维瘤病（6例）以及腹腔平滑肌肉瘤（15例），观察这些肿瘤中nestin的表达状况。结果96例CD117阳性的GISTs中，94例表达nestin，其中70例弥漫强表达，21例中度阳性，3例局灶阳性，仅2例阴性；5例CD117阴性的GISTs中，4例表达nestin；33例消化道雪旺瘤中26例表达nestin；15例平滑肌肉瘤中3例局灶表达nestin；10例食管平滑肌瘤、6例肠纤维瘤病均为阴性。结论nestin是辅助诊断GISTs的新指标，可鉴别GISTs与平滑肌肿瘤和纤维瘤病，但在GISTs与雪旺瘤的鉴别中需结合S-100蛋白等指标。     

类似胃肠间质瘤的胃梭形细胞间叶源性肿瘤31例临床病理分析

目的比较发生于胃的类似胃肠间质瘤(gastrointestinal stromal tumor, GIST)的梭形细胞间叶源性肿瘤,探讨其临床病理特征的关系。方法采用HE和免疫组化EnVision两步法检测31例与GIST类似的胃梭形细胞肿瘤,分析各类肿瘤的组织病理学特点、免疫表型及分子病理学特征。结果 31例类似GIST的胃梭形细胞间叶源性肿瘤,分别为平滑肌瘤14例,炎性纤维性息肉9例,神经鞘瘤5例,钙化性纤维性肿瘤1例,炎性肌纤维母细胞瘤1例,恶性周围神经鞘膜瘤1例。31例与GIST类似的梭形细胞肿瘤免疫组化均表达vimentin,不表达CD117和Dog-1;14例平滑肌瘤弥漫表达α-SMA和desmin;9例炎性纤维性息肉弥漫表达CD34;5例神经鞘瘤弥漫表达S-100,局部表达EMA;1例炎性肌纤维母细胞瘤表达α-SMA,局部表达CD34和actin;1例恶性周围神经鞘膜瘤弥漫表达NSE和SOX10,不表达S-100、CD99、CD34、EMA等。31例与GIST类似的梭形细胞肿瘤除1例恶性周围神经鞘膜瘤Ki-67增殖指数约20%,其余Ki-67增殖指数均10%。结论诊断类似GIST的胃梭形细胞间叶源性肿瘤,应综合考虑肿瘤的临床病理特点、免疫表型以及分子病理学特征,避免误诊。     

胃肠道间质瘤病理及CD117和CD34表达与意义

目的：对本院病案资料中GIST重新分类，加 强对胃肠道间质瘤的认识。方法：用CD117、CD34、α-SMA、S-100等抗 体对56例GIST进行标记、分类，根据GIST的临床病理及形态学特点，正确诊断，并探讨 GIST的临床特点。结果:56例GIST CD117、CD34弥漫强表达，抗体 阳性率分别为CD117（50/56，89.3%）、CD34（37/56，66.1%），部分病例局灶表达α-SM A、S-100，阳性率分别为（17/56，30.4%）、（4/56，7.1%），结蛋白desmin均阴性。其 中良性及交界性29例，恶性27例。本组病例中胃及小肠GIST共达91.1％，其它部位少见。 结论：CD117、CD34在GIST表达显著，可作为GIST诊断的一个辅助指标。 胃和小肠GIST最常见，胃镜等影像学无特异性，诊断主要依靠病理诊断。组织病理不仅有助 于诊断，而且有助于预后的判断。     

DOG1是一种敏感和特异性的胃肠道间质瘤的标记物

胃肠道间质瘤（GIST）是胃肠道最常见的间叶性肿瘤，大多数含有受体酪氨酸激酶基因c-KIT或PDGFRA的活化突变。GIST的诊断主要依赖于免疫组化KIT／CD117蛋白的表达，然而有4％～15％的GIST不表达CD117，导致诊断困难。DOG1是应用基因表达谱技术发现的一种高度表达于GIST的基因，作者采用2种新的鼠单克隆DOGI抗体（DOG1．1和DOG1．3），     

Gastrointestinal stromal tumors (GISTs): CD117, DOG-1 and PKCθ expression. Is there any advantage in using several markers?

Gastrointestinal stromal tumors (GISTs) are the most common mesenchymal tumors of the digestive tract. Expression of CD117, DOG1 and PKCθ was investigated immunohistochemically in a series of 99 paraffin-embedded GISTs in order to determine the sensitivity and diagnostic value of these markers. KIT exons 9, 11, 13 and 17 and PDGFRA exons 12 and 18 were amplified by PCR and sequenced. A total of 94/99 (94%) GISTs stained positive for CD117, 81/99 (82%) for PKCθ and 90/99 (91%) for DOG-1. A significant correlation was noted between CD117 and DOG-1 expression (p=0.0001). All three markers were expressed in 74% (73/99) of GISTs. Of the five CD117-negative cases, two were PKCθ-negative/DOG1-negative and had mutations in KIT exon 11. Two were PKCθ-positive/DOG1-positive and had mutations in PDGFRA (one each in exons 12 and 18), and one was DOG1-negative/PKCθ-positive, with a PDGFRA exon 18 mutation. The most sensitive marker was CD117, followed by DOG-1 and PKCθ. Although PKCθ was less sensitive, and its staining is more challenging and difficult to interpret, the use of this marker is highly recommended, particularly in CD117-negative/DOG-1-negative GISTs.     

Nestin and caveolin-1 in the diagnosis of GISTs

Gastrointestinal stromal tumors (GISTs) are the most common primary mesenchymal neoplasias of the gastrointestinal tract, typically expressing c-kit (CD117) and CD34. Recently, it was reported that nestin and caveolin-1 are also expressed in some human sarcomas, GISTs included. We performed a retrospective study on formalin fixed, paraffin embedded samples from 81 cases of confirmed GISTs, aiming to characterize their immunohistochemical profile, including nestin and caveolin-1 expressions. Tissue samples were evaluated immunohistochemically for CD117, CD34, nestin and caveolin-1. The patients (M:F 36:45), aged 46 to 84 years, had spindle cell type GISTs in 56.7% of cases, epithelioid in 30.8% and mixed pattern in 12.3%. Immunohistochemically, CD117 was positive in 88.9% of GISTs, CD34 in 85.1%, nestin in 77.7% and caveolin-1 in 71.6% of the tumors. Of nine c-kit negative GISTs, 66.7% expressed nestin, the same as caveolin-1 and 44.5% expressed both nestin and caveolin-1. Statistical analysis using Kendall's and Spearman's tests revealed significant correlations between nestin and caveolin-1 expressions (p=0.024). Our results suggest that nestin and caveolin-1 could be considered sensitive markers in GISTs, together with CD117 and CD34, for diagnostic purposes. Their significant expression in CD117 negative GISTs could represent an immunohistochemical alternative in establishing the diagnosis of these tumors.     

DOG1 and CD117 are the antibodies of choice in the diagnosis of gastrointestinal stromal tumours

Novelli M, Rossi S, Rodriguez‐Justo M, Taniere P, Seddon B, Toffolatti L, Sartor C, Hogendoorn P C W, Sciot R, Van Glabbeke M, Verweij J, Blay J Y, Hohenberger P, Flanagan A & Dei Tos A P
(2010) Histopathology 57 , 259–270
DOG1 and CD117 are the antibodies of choice in the diagnosis of gastrointestinal stromal tumours Aims: The histopathological diagnosis of gastrointestinal stromal tumours (GIST) is typically made based on a combination of clinical and morphological features supported by immunohistochemistry studies. The aim of this study was to examine the staining quality, sensitivity, specificity and utility of antibodies used commonly in GIST diagnosis. Methods and results: Immunohistochemistry with a panel of antibodies [CD117, DOG1, protein kinase C (PKC)‐theta, nestin, CD34, smooth muscle actin (SMA), desmin, S100 and CD171] was performed on whole sections from 187 GIST and 29 gastrointestinal mesenchymal tumours, and on several microarrays including 355 GISTs and 120 soft tissue sarcomas. Results showed that DOG1 and CD117 were the most sensitive and specific antibodies used in GIST diagnosis. PKC‐theta and nestin were sensitive, but less specific, also staining other spindle cell tumours commonly considered in the differential diagnosis of GIST. CD34 staining was less sensitive than many of the other antibodies and of limited aid in diagnosis. The smooth muscle markers SMA and desmin, together with the neural marker S100, were unhelpful in confirming a diagnosis of GIST, but were particularly useful in the exclusion/diagnosis of other gastrointestinal mesenchymal tumour types. Conclusions: In the majority of histologically suspected GISTs a combination of CD117 and DOG1 immunostaining is sufficient to confirm the histological diagnosis.     

Gastrointestinal Kaposi's sarcoma: CD117 expression and the potential for misdiagnosis as gastrointestinal stromal tumour

Aims:  Gastrointestinal Kaposi's sarcoma (KS) may mimic gastrointestinal stromal tumours (GISTs) histologically. Studies have shown that KS outside the gastrointestinal (GI) tract may express CD117, an antibody usually used to support a diagnosis of GIST. The aim was to evaluate the clinicopathological features of GI KS, including the expression of CD117 with and without antigen retrieval.
Methods and results:  Fourteen GI KS were assessed histologically, 12 of which were also subjected to immunohistochemistry for CD34, human herpesvirus (HHV) 8, DOG1 and CD117. CD117 immunohistochemistry was performed with and without antigen retrieval. All cases showed an infiltrative spindle cell tumour. Lamina propria infiltration, lymphoplasmacytic inflammation, extravasated red blood cells and haemosiderin were typical histological features. In all cases tumour cells were positive for CD34 and HHV8, but negative for DOG1. CD117 was positive in four of 12 cases without antigen retrieval and 10 of 12 cases with antigen retrieval.
Conclusions:  The microscopic distinction of GI KS from GIST can be difficult. Clues that raise the possibility of GI KS include young patient age, a history of immunosuppression, lamina propria infiltration, lymphoplasmacytic inflammation, extravasated red blood cells and haemosiderin deposition. Use of the immunomarkers CD117 (without antigen retrieval), HHV8 and DOG1 may aid in the distinction between GI KS and GIST.     

CD117阴性胃肠道间质瘤的临床病理及免疫组织化学研究

目的 探讨免疫组织化学在形态学典型、免疫组织化学CD117阴性胃肠道间质瘤(GIST)诊断中的意义.方法 对10例CD117阴性、形态学典型的GIST进行c-kit基因第9、11、13、17号外显子及血小板源性生长因子受体α(PDGFRA)基因第12和18号外显子的基因检测,同时所有病例均进行CD117、CD34、平滑肌肌动蛋白(SMA)、结蛋白、S-100蛋白、WT-1、DOG-1 的免疫组织化学染色(EnVision法).结果 10例中8例完成c-kit及PDGFRA基因的检测,仅1例有c-kit基因第9号外显子突变,余未发现基因突变.10例CD117阴性的病例9例CD34阳性,2例SMA局灶阳性.结蛋白和S-100蛋白均阴性.DOG1弥漫阳性者5例,1例弥漫弱阳性,2例局灶阳性,2例阴性.4例WT-1弥漫阳性,2例局灶阳性,1例有散在肿瘤细胞阳性,3例阴性.结论 对胃肠道及胃肠道外形态学典型、但CD117阴性的GIST病例,联合应用多种免疫组织化学标记有助于诊断.DOG-1和WT-1可作为补充加入到CD117阴性GIST的诊断中.     

CD117阴性胃肠道间质瘤的临床病理及免疫组织化学研究

目的 探讨免疫组织化学在形态学典型、免疫组织化学CD117阴性胃肠道间质瘤(GIST)诊断中的意义.方法 对10例CD117阴性、形态学典型的GIST进行c-kit基因第9、11、13、17号外显子及血小板源性生长因子受体α(PDGFRA)基因第12和18号外显子的基因检测,同时所有病例均进行CD117、CD34、平滑肌肌动蛋白(SMA)、结蛋白、S-100蛋白、WT-1、DOG-1 的免疫组织化学染色(EnVision法).结果 10例中8例完成c-kit及PDGFRA基因的检测,仅1例有c-kit基因第9号外显子突变,余未发现基因突变.10例CD117阴性的病例9例CD34阳性,2例SMA局灶阳性.结蛋白和S-100蛋白均阴性.DOG1弥漫阳性者5例,1例弥漫弱阳性,2例局灶阳性,2例阴性.4例WT-1弥漫阳性,2例局灶阳性,1例有散在肿瘤细胞阳性,3例阴性.结论 对胃肠道及胃肠道外形态学典型、但CD117阴性的GIST病例,联合应用多种免疫组织化学标记有助于诊断.DOG-1和WT-1可作为补充加入到CD117阴性GIST的诊断中.

Abstract：

Objective To study the immunophenotype and c-kit or platelet derived growth factor receptor alpha(PDGFRA)gene mutations in CD117-negative gastrointestinal stromal tumors(GISTs).Methods Ten cases of GISTs with typical histologic features but no CD117 expression were retrieved from the archival of Department of Pathology,Peking Union Medical College Hospital,China.The Cages were further evaluated for the presence of c-kit exons 9.11, 13 and 17 mutations and PDGFRA exons 12 and 18mutations.DNA was extracted from the paraffin-embedded tuinor tissue.The PCR products were sequenced directly for the mutations.An immunohistochemical study for CD117,CD34,smooth muscle actin,desmin,S-100 protein.WT-1 and DOC-1 Was also performed.Results Eight of the 10 Cases had the mutation tests completed.C-kit mumfion in exon 9 Wag detected in only one case.Amongst the 10 cases studied, CD34Wag expressed in 9 cases. Smooth muscle actin was focally positive in 2 cases.None of them expressed desmin or S-100 protein.DOG-1 and WT-1 were diffusely positive in 5 and 4 Cages.respectively.In addition.DOG1 Was diffusely but weakly positive in 1 case and focally expressed in 2 cages.Three cases were focally positive for WT-1.Conclusion Pathologic diagnosis of CD117-negative GISTs can be facilitated with the application of a panel of immunohistochemical markers.including DOG-1 and WT-1.     

DOG1 antibody is a highly sensitive and specific marker for gastrointestinal stromal tumors in cytology cell blocks

Initial diagnosis of submucosal gastrointestinal stromal tumors (GISTs) is often made from material obtained by endoscopic ultrasound-guided fine-needle aspiration (EUS-FNA). Although 95% of GISTs are positive for KIT by immunohistochemical analysis on surgical specimens, we have observed several cases of GIST that were negative for KIT on the cell block but subsequently positive on the surgical resection. DOG1 has been found to be a specific and sensitive marker for GISTs on surgical material. We compared KIT and DOG1 staining in 52 GIST cell blocks and in 44 cell blocks of other intra-abdominal spindle cell neoplasms. We found that DOG1 was the more sensitive marker, with positivity in all 52 GIST cell blocks. KIT was positive in 46 (88%) of the GIST cases, with sensitivity dependent on the FNA method. Both markers were highly specific: KIT was negative in all 44 non-GIST cases, whereas DOG1 showed weak positivity in only 1 leiomyosarcoma.     

The novel marker, DOG1, is expressed ubiquitously in gastrointestinal stromal tumors irrespective of KIT or PDGFRA mutation status

We recently characterized gene expression patterns in gastrointestinal stromal tumors (GISTs) using cDNA microarrays, and found that the gene FLJ10261 (DOG1, discovered on GIST-1), encoding a hypothetical protein, was specifically expressed in GISTs. The immunoreactivity of a rabbit antiserum to synthetic DOG1 peptides was assessed on two soft tissue tumor microarrays. The tissue microarrays included 587 soft tissue tumors, with 149 GISTs, including 127 GIST cases for which the KIT and PDGFRA mutation status was known. Immunoreactivity for DOG1 was found in 136 of 139 (97.8%) of scorable GISTs. All seven GIST cases with a PDGFRA mutation were DOG1-positive, while most of these failed to react for KIT. The immunohistochemical findings were confirmed with in situ hybridization probes for DOG1, KIT, and PDGFRA. Other neoplasms in the differential diagnosis of GIST, including desmoid fibromatosis (0 of 17) and Schwannoma (0 of 3), were immunonegative for DOG1. Only 4 of 438 non-GIST cases were immunoreactive for DOG1. DOG1, a protein of unknown function, is expressed strongly on the cell surface of GISTs and is rarely expressed in other soft tissue tumors. Reactivity for DOG1 may aid in the diagnosis of GISTs, including PDGFRA mutants that fail to express KIT antigen, and lead to appropriate treatment with imatinib mesylate, an inhibitor of the KIT tyrosine kinase.     

胃肠间质肿瘤免疫组化及细胞增殖活性研究

研究胃肠间质肿瘤的组织起源,分化以及细胞增殖。方法：应用ＬＳＡＢ法观察ＣＤ３４系系列标记物在２８例ＧＩＳＴｓ中的表达。结果：ＧＩＳＴｓ中ＣＤ３４和Ｖｉｍ的阳性率达８８．５％和９４％,且阳性程度强;肌源性和神经源性标记在部分阳性且相对偏弱;ＣＤ３４和ＰＣＮＡ在良恶性ＧＩＳＴｓ中表达无差异,Ｋｉ６７有一定区别。