重组人脑利钠肽治疗慢性充血性心力衰竭的疗效观察

目的观察重组人脑利钠肽治疗慢性充血性心力衰竭的临床疗效。方法 68例充血性心力衰竭患者,随机分成治疗组36例和对照组32例,2组均给予强心、常规利尿、扩血管、β受体阻滞剂和(或)ACEI类药物治疗,治疗组在常规治疗基础上加用重组人脑利钠肽治疗,观察2组治疗前后临床状况好转率、心率、尿量、左室射血分数(LVEF)等情况。结果治疗组的治疗总有效率为91.7%优于对照组的78.2%,差异有统计学意义(P<0.05);治疗组心率改善、尿量改善及心功能改善等高于对照组,差异有统计学意义(P<0.05)。结论重组人脑利钠肽能明显改善慢性充血性心力衰竭患者的临床症状。     

重组人脑利钠肽治疗充血性心力衰竭的疗效评价

目的探讨选择重组人脑利钠肽对充血性心力衰竭患者完成治疗后获得的临床效果。方法将我院2013年12月至2015年12月收治的充血性心力衰竭患者110例作为此次实验的研究对象。研究采用随机分组对比的形式,通过随机数表法展开。对55例观察组充血性心力衰竭患者临床采用重组人脑利钠肽实施治疗;对55例对照组充血性心力衰竭患者临床采用常规治疗的方法;观察对比临床相关指标水平以及治疗总有效率等。结果两组充血性心力衰竭患者完成治疗后,在LVWP水平、LVD水平以及HR水平几方面,同治疗前比较,获得显著降低(P<0.05);患者的LVEF水平获得显著提高(P<0.05)。组间比较,观察组明显优于对照组充血性心力衰竭患者(P<0.05)。结论对于充血性心力衰竭患者,临床采用重组人脑利钠肽的方法进行治疗,患者出现不良反应的概率较低,临床获得的有效率较高,体现出较高的治疗安全性。     

厄贝沙坦联合重组人脑利钠肽治疗充血性心力衰竭的疗效及安全性

目的研究厄贝沙坦与重组人脑利钠肽联合治疗充血性心力衰竭患者的效果及安全性。方法选取72例充血性心力衰竭患者进行研究,收治时间为2014年7月至2016年3月,随机分为对照组和观察组,各36例。两组均进行常规对症治疗,对照组在此基础上使用厄贝沙坦治疗,观察组在上述基础上采取重组人脑利钠肽进行联合治疗。观察两组患者的临床效果、不良反应发生情况以及治疗前后血清指标。结果观察组患者治疗后的总有效率(86.11%)高于对照组患者,P值<0.05;两组患者不良反应发生情况对比没有较大差异,P值>0.05;在血清指标水平方面,观察组患者治疗后的数据比对照组患者更低,P值<0.05。结论厄贝沙坦与重组人脑利钠肽联合治疗充血性心力衰竭患者效果显著,能够明显降低相关血清指标。     

应用重组人脑利钠肽治疗充血性心力衰竭57例临床疗效观察

目的观察重组人脑利钠肽治疗充血性心力衰竭的临床疗效。方法收集符合充血性心力衰竭诊断标准、应用人脑利钠肽治疗患者57例,以同期未用此药治疗的该疾病患者63例作为对照。在常规治疗基础上,治疗组加用重组人脑利钠肽治疗3天后进行临床疗效分析。结果治疗组和对照组有效率分别为94.74%和82.54%,经检验差别有统计学意义（P〈0.05）。结论重组人脑利钠肽治疗充血性心力衰竭临床效果明显。     

注射用重组人脑利钠肽联合呋塞米注射液治疗急性心力衰竭患者的临床疗效观察

目的 探讨注射用重组人脑利钠肽联合呋塞米注射液治疗急性心力衰竭患者的临床疗效.方法 100例急性心力衰竭患者,随机分为观察组与对照组,每组50例.对照组患者接受呋塞米注射液治疗,观察组患者在对照组基础上联合注射用重组人脑利钠肽治疗.比较两组患者心功能相关生化指标[脑利钠肽(BNP)、血肌酐(SCR)、血钾(K+)、血钠...     

重组人脑利纳肽和乌拉地尔治疗急性充血性心力衰竭的临床效果对比观察

目的探讨重组人脑利钠肽和乌拉地尔治疗急性充血性心力衰竭的临床疗效。方法选择2012年8月至2013年8月我院心内科住院的急性充血性心力衰竭患者80例,随机分为观察组和对照组各40例,在给予常规强心利尿扩血管治疗基础上,分别予重组人脑利钠肽和乌拉地尔治疗,观察两组治疗效果及不良反应发生情况。结果 (1)经治疗后观察组总有效率77.5%(31/40),对照组总有效率55%(22/40),两组比较差异有统计学意义(P<0.05)。(2)两组在不良反应发生率上比较差异无统计学意义(P>0.05)。结论采用重组人脑利钠肽治疗急性充血性心力衰竭相比乌拉地尔效果好,可明显改善患者血流动力学,应用安全可靠,值得临床上推广。     

重组人脑利钠肽联合去乙酰毛花苷治疗急性心力衰竭的临床研究

目的探究重组人脑利钠肽联合去乙酰毛花苷治疗急性心力衰竭患者的临床疗效和安全性。方法选取2014年1月—2017年1月在昌江黎族自治县人民医院治疗的急性心力衰竭患者150例,随机分成对照组(75例)和治疗组(75例)。对照组患者静脉滴注去乙酰毛花苷注射液,0.4 mg溶于5%葡萄糖注射液10 m L中,10 min内滴注完毕,然后每2小时静脉滴注一次,直至患者急性左心衰症状完全缓解;治疗组患者在对照组的基础上静脉推注注射用重组人脑利钠肽,1.5μg/kg负荷剂量,然后采用微量泵以0.01μg/(kg·min)持续泵入。两组患者均治疗48 h。观察两组患者临床疗效,同时比较治疗前后两组患者收缩压、心室率、左心衰缓解时间、超敏C反应蛋白(hs-CRP)、同型半胱氨酸(Hcy)和N-末端脑钠肽前体(NT-pro BNP)水平及不良反应情况。结果治疗后,对照组和治疗组的总有效率分别为82.67%、94.67%,两组比较差异具有统计学意义(P0.05)。治疗后,两组患者的收缩压、心室率均显著降低(P0.05),且治疗组收缩压、心室率和左心衰缓解时间均显著低于对照组患者(P0.05)。治疗后,两组患者hs-CRP、Hcy和NT-pro BNP血清水平均显著降低(P0.05),且治疗后治疗组患者hs-CRP、Hcy和NT-pro BNP血清水平明显低于对照组(P0.05)。治疗期间,治疗组患者不良反应的发生率为4.00%,显著低于对照组的14.67%,两组比较差异具有统计学意义(P0.05)。结论重组人脑利钠肽联合去乙酰毛花苷治疗急性心力衰竭疗效显著,不良反应发生率低,具有一定的临床推广应用价值。     

生脉注射液联合重组人脑利钠肽治疗急性心力衰竭的临床研究

目的探讨生脉注射液联合重组人脑利钠肽治疗急性心力衰竭的临床效果。方法选取2014年1月—2017年10月在中国人民解放军第二五四医院收治的112例急性心力衰竭患者,随机分为对照组和治疗组,每组各56例。对照组静脉滴注注射用重组人脑利钠肽,首次静脉冲击1.5μg/kg,随后按照0.01μg/(kg·min)静脉泵入。治疗组在对照组治疗基础上静脉滴注生脉注射液,50 m L生脉注射液加入到5%葡萄糖注射液100 m L,1次/d。两组均连续治疗3 d。观察两组的临床疗效,比较两组治疗前后左心室射血分数(LVEF)、左室舒张末内径(LVEDD)、尿素氮(BUN)、肌酐(Cr)、血清脑钠肽(BNP)的变化情况。结果治疗后,对照组和治疗组的总有效率分别为78.57%、94.64%,两组比较差异有统计学意义(P0.05)。治疗后,两组患者LVEF显著升高,但LVEDD、BUN、Cr、BNP显著降低,同组治疗前后比较差异有统计学意义(P0.05);治疗后,治疗组LVEF高于对照组,LVEDD、BUN、Cr、BNP低于对照组,两组比较差异具有统计学意义(P0.05)。结论生脉注射液联合重组人脑利钠肽治疗急性心力衰竭具有显著的临床治疗效果,可明显改善患者心功能,保护肾功能,具有一定的临床推广应用价值。     

冻干重组人脑利钠肽、米力农治疗充血性心力衰竭疗效对比

目的：观察冻干重组人脑利钠肽、米力农治疗充血性心力衰竭的疗效。方法：将62例充血性心力衰竭患者随机分为两组,一组（对照组）在常规治疗的基础上加用米力农,另一组（观察组）在常规治疗的基础上加用冻干重组人脑利钠肽,观察治疗前后两组患者的临床症状、体征、心功能等的改善情况。结果：两组治疗前后,观察组疗效明显优于对照组,两者比较,差异有统计学意义。结论：冻干重组人脑利钠肽治疗充血性心力衰竭疗效确切,较米力农改善更加显著。     

益心舒胶囊联合重组人脑利钠肽治疗慢性充血性心力衰竭的临床研究

目的探讨益心舒胶囊联合重组人脑利钠肽治疗慢性充血性心力衰竭的临床疗效和安全性。方法选取2014年2月—2015年12月延安市人民医院收治的慢性充血性心力衰竭患者112例,随机分为对照组和治疗组,每组各56例,对照组患者静脉滴注冻干重组人脑利钠肽,首次静脉冲击剂量1.5μg/kg,之后以7.5μg/(kg·min-1)的速度静脉滴注。治疗组患者在对照组患者的治疗基础上口服益心舒胶囊,3粒/次,3次/d。两组均连续治疗14 d。观察两组的临床疗效,比较患者左心室收缩末期内径(LVESD)、左室舒张末期内径(LVEDD)、左室内径(LADD)、心指数(CI)、左室射血分数(LVEF)等指标,检测治疗前后去甲肾上腺素(NE)、内皮素-1(ET-1)和抗利尿激素(ADH)指标,并观察两组患者不良反应发生情况。结果治疗后,对照组和治疗组的总有效率分别为78.57%、91.07%,两组比较差异具有统计学意义(P0.05);两组患者LVESD、LVEDD、LADD明显降低,CI和LVEF明显升高,同组治疗前后差异有统计学意义(P0.05);与对照组相比,治疗组患者观察指标改善更明显,差异具有统计学意义(P0.05)。治疗后,两组患者NE、ADH、ET-1水平显著下降,同组治疗前后差异有统计学意义(P0.05);且治疗组患者NE、ADH和ET-1水平改善程度更优于对照组,两组比较差异有统计学意义(P0.05)。结论益心舒胶囊联合重组人脑利钠肽治疗慢性充血性心力衰竭具有较好的临床疗效,安全性高,具有一定的临床推广应用价值。     

Synthesis,Cytotoxicity and Antileishmanial Activity of Some N-(2-(indol-3-yl)ethyl)-7-chloroquinolin-4-amines

We report herein the condensation of 4,7-dichloroquinoline (1) with tryptamine (2) and D-tryptophan methyl ester (3) . Hydrolysis of the methyl ester adduct (5) yielded the free acid (6) . The compounds were evaluated in vitro for activity against four different species of Leishmania promastigote forms and for cytotoxic activity against Kb and Vero cells. Compound (5) showed good activity against the Leishmania species tested, while all three compounds displayed moderate activity in both Kb and Vero cells.     

Efficacy of gut lavage,hemodialysis, and hemoperfusion in the therapy of paraquat or diquat intoxication

Clinical and in vitro investigations were carried out to test the efficacy of gut lavage, hemodialysis, and hemoperfusion in the treatment of poisoning with paraquat or diquat. In a patient suffering from diquat intoxication 130 times more diquat was removed by gut lavage 30 h after ingestion than was removed by complete aspiration of the gastric contents.Determination of in vitro clearances for paraquat and diquat by hemodialysis showed that, at serum concentrations of 1–2 ppm, such as are frequently encountered in poisoning in man, toxicologically relevant quantities of herbicide cannot be removed from the body. At a concentration of 20 ppm, on the other hand, hemodialysis proved to be effective, the clearance being 70 ml/min at a blood flow rate of 100 ml/min. The efficacy of hemoperfusion with coated activated charcoal was on the whole better. Especially at concentrations around 1–2 ppm, the clearance values for hemoperfusion were some 5–7 times higher than those for hemodialysis.In a patient suffering from paraquat poisoning, both hemodialysis as well as hemoperfusion were carried out. The in vitro results could be confirmed: At serum concentrations of paraquat less than 1 ppm no clearance could be obtained by hemodialysis while by hemoperfusion with activated charcoal quite high clearance values were measured and the serum level dropped down to zero.

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Zusammenfassung Klinische Untersuchungen und Laboratoriumsversuche wurden durchgeführt, um die Wirksamkeit von Darmspülung, Hämodialyse und Hämoperfusion bei Paraquat- und Deiquat-Vergiftungen zu prüfen.Bei einem Patienten wurde 30 Std nach Deiquat-Aufnahme durch Darmspülung 130mal mehr Deiquat entfernt als durch vollständige Aspiration des Mageninhaltes. In vitro-Versuche ergaben, daß bei Blutserumkonzentrationen von 1–2 ppm, die bei Vergiftungen oft gemessen werden, durch Hämodialyse keine toxikologisch relevanten Paraquat- oder Deiquat-Mengen entfernt werden können. Dagegen erwies sich die Hämodialyse bei 20 ppm und einer Blutumlaufgeschwindigkeit von 100 ml/min mit einer Clearance von 70 ml/min als wirksam. Die Hämoperfusion mit beschicheter Aktivkohle war in diesen Versuchen aber eindeutig überlegen, denn insbesondere bei Konzentrationen um 1–2 ppm waren die Clearance-Werte 5–7mal höher als bei der Hämodialyse.Die in vitro-Ergebnisse wurden bei einem Patienten mit einer Paraquat-Vergiftung bestätigt: Bei Konzentrationen unter 1 ppm war die Hämodialyse wirkungslos, während durch Hämoperfusion relativ hohe Clearance-Werte erreicht wurden, so daß der Serumspiegel rasch unter die Nachweisgrenze abfiel.

     

In vitro studies on sterically stabilized liposomes (SL) as enzyme carriers in organophosphorus (OP) antagonism

This study describes a new approach for organophosphorous (OP) antidotal treatment by encapsulating an OP hydrolyzing enzyme, OPA anhydrolase (OPAA), within sterically stabilized liposomes. The recombinant OPAA enzyme was derived from Alteromonas strain JD6. It has broad substrate specificity to a wide range of OP compounds: DFP and the nerve agents, soman and sarin. Liposomes encapsulating OPAA (SL)* were made by mechanical dispersion method. Hydrolysis of DFP by (SL)* was measured by following an increase of fluoride ion concentration using a fluoride ion selective electrode. OPAA entrapped in the carrier liposomes rapidly hydrolyze DFP, with the rate of DFP hydrolysis directly proportional to the amount of (SL)* added to the solution. Liposomal carriers containing no enzyme did not hydrolyze DFP. The reaction was linear and the rate of hydrolysis was first order in the substrate. This enzyme carrier system serves as a biodegradable protective environment for the recombinant OP-metabolizing enzyme, OPAA, resulting in prolongation of enzymatic concentration in the body. These studies suggest that the protection of OP intoxication can be strikingly enhanced by adding OPAA encapsulated within (SL)* to pralidoxime and atropine.     

Lung disease and PKCs

The lung offers a rich opportunity for development of therapeutic strategies focused on isozymes of protein kinase C (PKCs). PKCs are important in many cellular responses in the lung, and existing therapies for pulmonary disorders are inadequate. The lung poses unique challenges as it interfaces with air and blood, contains a pulmonary and systemic circulation, and consists of many cell types. Key structures are bronchial and pulmonary vessels, branching airways, and distal air sacs defined by alveolar walls containing capillaries and interstitial space. The cellular composition of each vessel, airway, and alveolar wall is heterogeneous. Injurious environmental stimuli signal through PKCs and cause a variety of disorders. Edema formation and pulmonary hypertension (PHTN) result from derangements in endothelial, smooth muscle (SM), and/or adventitial fibroblast cell phenotype. Asthma, chronic obstructive pulmonary disease (COPD), and lung cancer are characterized by distinctive pathological changes in airway epithelial, SM, and mucous-generating cells. Acute and chronic pneumonitis and fibrosis occur in the alveolar space and interstitium with type 2 pneumocytes and interstitial fibroblasts/myofibroblasts playing a prominent role. At each site, inflammatory, immune, and vascular progenitor cells contribute to the injury and repair process. Many strategies have been used to investigate PKCs in lung injury. Isolated organ preparations and whole animal studies are powerful approaches especially when genetically engineered mice are used. More analysis of PKC isozymes in normal and diseased human lung tissue and cells is needed to complement this work. Since opposing or counter-regulatory effects of selected PKCs in the same cell or tissue have been found, it may be desirable to target more than one PKC isozyme and potentially in different directions. Because multiple signaling pathways contribute to the key cellular responses important in lung biology, therapeutic strategies targeting PKCs may be more effective if combined with inhibitors of other pathways for additive or synergistic effect. Mechanisms that regulate PKC activity, including phosphorylation and interaction with isozyme-specific binding proteins, are also potential therapeutic targets. Key isotypes of PKC involved in lung pathophysiology are summarized and current and evolving therapeutic approaches to target them are identified.     

Steroidal sapogenin contents in some domestic plants

In order to find out the values of the steroid resources for the future use. the compositions and contents of steroidal sapogenins from 13 domestic plants have been investigated. As a result,Dioscorea nipponica, D. quinqueloba andSmilax china were found to have large amount of diosgenin. And pennogenin inTrillium kamtschaticum andParis verticillata, yuccagenin inAllium fistulosum, hecogenin inAgave americana and neochlorogenin inSolanum nigum were appeared to be major steroidal sapogenins.