GC-MS分析宁夏苦豆子不同部位挥发油的化学成分

目的利用气相色谱-质谱联用技术(GC-MS)测定苦豆子不同部位的挥发油成分,并比较各成分。方法利用水蒸汽蒸馏法提取挥发油,乙醚萃取所得部分通过GC-MS分析;根据所得色谱保留时间和纯质谱,在质谱库中进行相似性检索,实现对组分的鉴定,同时用峰面积归一化法计算其相对含量。结果从苦豆子的种子、根、叶和茎中分别得到30、12、15、12个挥发油成分,种子中以酚类和萜类化合物为主要成分,根、叶和茎都以酯类化合物为主要成分。结论苦豆子不同部位挥发油的成分组成和含量有较大差别。     

微波辅助提取与水蒸气蒸馏杭白芷挥发油的比较

目的:比较微波辅助提取与水蒸气蒸馏杭白芷挥发油的提取率和化学成分。方法:分别采用微波辅助提取和水蒸气蒸馏提取白芷挥发油,并运用毛细管气相色谱-质谱联用法结合计算机检索对其挥发油进行化学成分分析。结果:共鉴定出68个成分,从微波辅助提取和水蒸气蒸馏提取所得挥发油中分别鉴定出了59种和37种化合物,分别占总峰面积的88.93%和94.79%。结论:微波辅助提取与水蒸气蒸馏提取所得挥发油的化学成分有较大差异,微波提取的挥发油成分更多。     

比较研究新疆狭叶薰衣草不同部位挥发油成分

目的:对狭叶薰衣草花、叶及茎中挥发油的化学成分进行分析,为狭叶薰衣草的综合利用提供指导。方法:采集狭叶薰衣草的花、叶及茎,以水蒸气蒸馏法提取挥发油,以GC-MS法分析其化学成分。GC-MS主要分析条件:进样口温度250℃,分流比30∶1,离子源温度200℃,程序升温(在40℃保持2 min,最后以3℃·min-1升温速率升至246℃,保持5 min)。结果:在狭叶薰衣草的花、叶及茎的挥发油中分别检出80、60和16个色谱峰,分别鉴定出69、47、15个化合物,分别占各部位挥发油组分总色谱峰面积的94.01%、86.83%、97.35%,3个部位中共有化合物15个。所含成分多为萜烯类化合物及其含氧衍生物。结论:狭叶薰衣草花中挥发油成分含量最多,茎中成分最少,叶挥发油具有潜在的应用价值。     

鼠曲草挥发油化学成分的GC—MS分析

目的 鉴定鼠曲草挥发油的化学成分。方法 用常规的水蒸气蒸馏法提取鼠曲草挥发油,采用气相色谱）质谱联用仪分析其挥发油的化学成分。结果 共分离出15种成分,鉴定出11种成分,所鉴定的组分占挥发油色谱总峰面积的73．33％。结论 鼠曲草挥发油的主要成分为石竹烯。     

托里桉叶挥发油化学成分的气相色谱-质谱联用分析

目的研究广东托里桉叶挥发油的化学成分。方法 采用水蒸气蒸馏法提取挥发油,用气相色谱-质谱联用技术进行分离检定,对分离的化合物进行结构鉴定,应用气相色谱峰面积归一化法确定各组成分的相对含量。结果确定了广东托里桉叶挥发油中30个化合物,其主要成分为α-松油醇(35.77%),其次是苯甲醛(5.48%)等。占色谱峰总流出面积的80.68%。结论首次确定了广东托里桉叶挥发油的30个化合物,为综合利用奠定了基础。     

蒙药旋覆花挥发油化学成分分析

目的:利用气相色谱-质谱联用技术对蒙药旋覆花挥发油化学成分进行全面分析。方法:使用乙醚萃取浓缩、水蒸气蒸馏的方法提取旋覆花挥发油,利用气相色谱-质谱联用技术分离鉴定,采用 GC 峰面积归一化法定量。结果:共鉴定出29个成分,占挥发油色谱总峰面积的90.35%,其中邻苯二甲酸二丁基酯16.30%,有6个化合物含量超过7%。结论:本方法快速方便,重现性好,适用于旋覆花挥发油化学成分分析。     

托里桉叶挥发油化学成分的气相色谱-质谱联用分析

目的 研究广东托里桉叶挥发油的化学成分.方法 采用水蒸气蒸馏法提取挥发油,用气相色谱-质谱联用技术进行分离检定,对分离的化合物进行结构鉴定,应用气相色谱峰面积归—化法确定各组成分的相对含量.结果 确定了广东托里桉叶挥发油中30个化合物,其主要成分为α-松油醇(35 77％),其次是苯甲醛(5 48％)等.占色谱峰总流出...     

猫眼草挥发油成分的GC-MS分析

目的研究猫眼草全草的挥发油成分。方法采用水蒸气蒸馏法提取猫眼草的挥发油,利用气相色谱/质谱联用技术(GC-MS)对挥发油中的化学成分进行分离分析,并采用峰面积归一化法计算各成分相对百分含量。结果对其中的43种化学成分进行了鉴定,所鉴定的成分占总流出峰面积的75.86%。其中醛类化合物14种,占30.77%;酮类8种,占19.02%;醇类14种,占17.08%。结论本文首次分析了猫眼草全草挥发油成分,为了解猫眼草的化学物质基础和进一步开发研究提供了依据。     

广陈皮的超临界流体萃取和水蒸气蒸馏挥发油的比较分析

目的:比较两种不同提取方法所得的广陈皮挥发油的挥发性化学成分。方法:分别采用超临界CO2流体萃取(SFE)和水蒸气蒸馏(SD)的方法提取广陈皮的挥发油。运用气相色谱-质谱联用法分离和分析两挥发油的挥发性化学成分,主要成分棕榈酸和D-柠檬烯采用标准品的峰面积增大法进一步确认。结果:从广陈皮SFE和SD挥发油中共鉴定出22个成分,占色谱总流出峰面积的97%以上,两者的提取率分别为5.05%和0.46%。SFE挥发油的主要成分为挥发性较弱、相对分子量较大的棕榈酸,SD挥发油的主要成分为挥发性较强、相对分子量较小的D-柠檬烯。此外,SFE和SD挥发油中均有含量不低的2-甲胺基-苯甲酸甲酯。结论:采用两种方法提取广陈皮所得挥发油的挥发性成分不尽相同,且SFE法的提取率高于SD法;两种方法均能将广陈皮的特征性成分2-甲胺基-苯甲酸甲酯提取出来。     

大罗伞鲜品根、茎、叶挥发油成分的GC-MS分析

目的:研究大罗伞鲜品根、茎、叶中的挥发油成分。方法:采摘12 h内的大罗伞,用挥发油测定器提取挥发油,用气相色谱-质谱联用技术对挥发油成分进行分析,采用NIST标准谱库结合保留指数解谱法对各成分进行定性分析,并用面积归一化法计算各成分的质量分数。结果:从大罗伞根、茎、叶的挥发油中确定出7、12、5个成分,分别占各自挥发油总量的91.7%、83.6%、85.4%;其中,棕榈酸为大罗伞根、茎、叶挥发油的主要成分,分别占各自挥发油总量的66.0%、60.4%、71.8%。结论:该方法可快速、准确地鉴别大罗伞鲜品根、茎、叶中的挥发油成分,可为大罗伞药材的进一步开发利用提供科学依据。     

伏康树籽挥发油的GC-MS分析

目的:分析伏康树籽的挥发油成分。方法:采用水蒸气蒸馏法提取伏康树籽挥发油,通过气相色谱-质谱联用技术对其成分进行鉴定,同时采用面积归一法测定其含量。结果:分离出32种物质,鉴定出22个化合物,占总馏出组分的97.85%,其中脂肪酸类百分含量为80.93%。结论:伏康树籽挥发油主要成分为油酸(30.46%),棕榈酸(28.65%),亚油酸(12.31%)等。通过对伏康树籽挥发油成分的分析鉴定,为进一步开发利用其资源提供科学依据。     

Essential oil of the Persian sage, Salvia rhytidea Benth

Chemical composition of volatile compounds from Salvia rhytidea Benth. was analyzed, for the first time, by gas chromatography/mass spectrometry. The volatiles were isolated from dried aerial parts of the plant by hydrodistillation. A total yield of 2.0 mg of essential oil per g of plant dry mass was obtained and sixty compounds were identified, representing 98.2% of total volatiles. The essential oil was characterized by a high content of hydrocarbon and oxygenated monoterpenes. The main constituents were p-cymene-8-ol (11.9%), spathulenol (7.3%), pulegone (6.4%), sabinene (5.8%), terpinen-4-ol (5.5%) and alpha-copaene (5.3%).     

Composition,antimicrobial activity and in vitro cytotoxicity of essential oil from Cinnamomum zeylanicum Blume (Lauraceae)

The essential oil from the bark of Cinnamomum zeylanicum Blume was analyzed by GC–MS and bioassays were carried out. Nine constituents representing 99.24% of the oil were identified by GC–MS. The major compounds in the oil were (E)-cinnamaldehyde (68.95%), benzaldehyde (9.94%) and (E)-cinnamyl acetate (7.44%). The antimicrobial activity of the oil was investigated in order to evaluate its efficacy against 21 bacteria and 4 Candida species, using disc diffusion and minimum inhibitory concentration methods. The essential oil showed strong antimicrobial activity against all microorganisms tested. The cytotoxic and apoptotic effects of the essential oil on ras active (5RP7) and normal (F2408) fibroblasts were examined by MTT assay and acridine orange/ethidium bromide staining, respectively. The cytotoxicity of the oil was quite strong with IC₅₀ values less than 20 μg/mL for both cell lines. 5RP7 cells were affected stronger than normal cells. Morphological observation of apoptotic cells indicated the induction of apoptosis at the high level of the oil, especially in 5RP7 cells. The present study showed the potential antimicrobial and anticarcinogenic properties of the essential oil of cinnamon bark, indicating the possibilities of its potential use in the formula of natural remedies for the topical treatment of infections and neoplasms.     

蚧螬化学成分的GC-MS分析

目的分析蚧螬石油醚提取物的化学成分。方法采用石油醚超声提取挥发油,气相色谱-质谱-计算机联用技术分析。结果共鉴定出21种成分,占挥发油总量的96.3%。结论蚧螬石油醚提取物的主要成分是反油酸50.82%,十六酸25.64%,反-11-十六碳烯酸8.98%,硬脂酸2.65%。     

Composition and antimicrobial activity of the essential oil of Achillea multifida

The composition of the water-distilled essential oil of Achillea multifida (DC.) Boiss. (Compositae) was analysed by GC and GC/MS. Fifty-eight compounds were identified representing 93.9 % of the total oil. alpha-Thujone (60.9 %), beta-thujone (9.1 %), sabinene (4.1 %) and camphor (3.7 %) were characterised as the main constituents. The essential oil was tested for its antimicrobial activity using a micro-dilution assay resulting in the inhibition (MIC: 62.5-250 microg/ml) of human pathogenic bacteria and yeast.     

Volatile Constituents of Peucedanum palustre

The essential oils of fruits, stalks, leaves, and roots of PEUCEDANUM PALUSTRE (L.) Moench (Apiaceae) were analyzed by various chromatographic and spectroscopic methods. While the fruit and stalk oils consist nearly exclusively of monoterpene hydrocarbons, the essential leaf oil comprises monoterpene hydrocarbons (47%), sesquiter-pene hydrocarbons (38%), aliphatic alcohols and esters (7%), and traces of oxygenated sesquiterpenoids. 35 constituents of this oil could be identified. The detailed analysis of the complex root oil, mainly consisting of mono- and sesquiterpenoids, resulted in identification of 68 compounds, representing about 92% of the oil. The occurrence of some new sesquiterpenoids with unusual carbon skeletons is noteworthy.     

不同白术栽培品种挥发油化学成分的GC-MS分析

目的:对不同白术栽培品种挥发油化学成分进行比较分析。方法:采用水蒸气蒸馏法提取白术挥发油,用气相色谱-质谱(GC-MS)联用技术对不同白术栽培品种的挥发油化学成分进行测定,用面积归一化法计算其相对含量。结果:鉴定出主流白术主要化学成分27种,占总化合物的84.24%;鉴定出2个变异白术化学成分分别为37种和35种,分别占总化合物的91.92%和94.72%。主流品种主要含苍术酮;变异品种主要含茅苍术醇、β-桉油醇和苍术酮,富含倍半萜醇类。结论:本研究结果可为白术药材的质量评价和控制提供依据。     

Chemical Composition and Antimicrobial Activity of the Volatile Oil of Artemisia khorassanica. from Iran

Abstract

Artemisia khorassanica. Podl. (Asteraceae) is a common perennial herb growing wild in northeastern parts of Iran. The essential oil of A. khorasanica. was isolated by hydrodistillation in 1.25 (v/w) yield. The chemical composition of the essential oil was examined by gas chromatography and gas chromatography-mass spectrometry. Thirty-one compounds were identified, representing 79.6% of the total oil. The major constituents were 1,8-cineol (17.7%), camphor (13.9%), davanone (12.2%), and isogeraniol (5.7%). Minimum inhibitory concentration was determined using agar dilution method against eight bacteria and two fungal strains. The essential oil indicated a moderate antimicrobial activity.     

二氧化碳超临界流体萃取两面针根挥发油成分的气相色谱-质谱分析

目的分析两面针根挥发油的化学成分,为两面针药材的综合开发应用提供科学实验依据。方法采用二氧化碳超临界流体萃取两面针根挥发油成分,用气相色谱毛细管柱进行分离,质谱检测,归一化法测定其相对含量。结果检出34个成分,鉴定了23个化合物,占挥发油总量的95.12%。两面针根的挥发油主要化学成分为萜类,其中荜澄茄烯醇相对含量高达49.96%,其次是亚麻油酸和亚油酸,含量分别为14.34%和10.16%。结论所用方法所得结果与文献报道的差异较大,可能与产地、采收期、提取方法等因素有关。