危重症患者血清降钙素原测定对细菌感染的诊断价值

Objective To evaluate the clinical value of serum procalcitonin (PCT) in the diagnosis of bacte-rial infections in the critically ill patients. Methods A loud d 100 cases of critical patients were divided into bacteri-al infection group(56 cases) and non-bacterial infection group(44 cases). Serum PCT was measured by immunolu-minometric assay. Results The concentration of PCT in bacterial infection group(21.54 ±5.72) μg/L, was signifi-cantly higher than non-bacterial infection group (11. 95±4. 58)μg/L (t =2.291,P<0.05);The APACHE Ⅲ score of bacterial infection group(62. 44 ± 19. 55) cent was significantly higher than non-bacterial infection group(44. 56 ± 25. 88) cent(t = 2. 195 ,P < 0. 05). The concentration of PCT of 1.0μg/L and 2. 0 p.g/L compared to the former sensitivity (96. 5) % higher than the latter (55.2) % (X2 = 3. 94, P < 0. 05), the former specificity (41.7) % lower than the latter (95.8)% (X2 = 4. 02 ,P < 0. 05);1.5μg/L as a positive standard Youden index and the Agreement (83.0 % ,0. 65), were significantly higher than 1 μg/L and 2 μg/L(71.7%, 0. 38 ;73.6% ,0. 51) (X2 = 3.84, X2=3. 90,X2 = 3.992 = 3.91 ,P < 0. 05);The concentration of PCT in death group (38. 9 ±12. 6)μg/L was signifi-canfly higher than the survival group(11.8± 8. 3) μg/L(t =2. 398 ,P <0. 05). Conclusion Serum PCT has clinical values in the diagnosis and therapy of bacterial infections in the critical patients.     

危重症患者血清降钙素原测定对细菌感染的诊断价值

Objective To evaluate the clinical value of serum procalcitonin (PCT) in the diagnosis of bacte-rial infections in the critically ill patients. Methods A loud d 100 cases of critical patients were divided into bacteri-al infection group(56 cases) and non-bacterial infection group(44 cases). Serum PCT was measured by immunolu-minometric assay. Results The concentration of PCT in bacterial infection group(21.54 ±5.72) μg/L, was signifi-cantly higher than non-bacterial infection group (11. 95±4. 58)μg/L (t =2.291,P<0.05);The APACHE Ⅲ score of bacterial infection group(62. 44 ± 19. 55) cent was significantly higher than non-bacterial infection group(44. 56 ± 25. 88) cent(t = 2. 195 ,P < 0. 05). The concentration of PCT of 1.0μg/L and 2. 0 p.g/L compared to the former sensitivity (96. 5) % higher than the latter (55.2) % (X2 = 3. 94, P < 0. 05), the former specificity (41.7) % lower than the latter (95.8)% (X2 = 4. 02 ,P < 0. 05);1.5μg/L as a positive standard Youden index and the Agreement (83.0 % ,0. 65), were significantly higher than 1 μg/L and 2 μg/L(71.7%, 0. 38 ;73.6% ,0. 51) (X2 = 3.84, X2=3. 90,X2 = 3.992 = 3.91 ,P < 0. 05);The concentration of PCT in death group (38. 9 ±12. 6)μg/L was signifi-canfly higher than the survival group(11.8± 8. 3) μg/L(t =2. 398 ,P <0. 05). Conclusion Serum PCT has clinical values in the diagnosis and therapy of bacterial infections in the critical patients.     

危重症患者血清降钙素原测定对细菌感染的诊断价值

Objective To evaluate the clinical value of serum procalcitonin (PCT) in the diagnosis of bacte-rial infections in the critically ill patients. Methods A loud d 100 cases of critical patients were divided into bacteri-al infection group(56 cases) and non-bacterial infection group(44 cases). Serum PCT was measured by immunolu-minometric assay. Results The concentration of PCT in bacterial infection group(21.54 ±5.72) μg/L, was signifi-cantly higher than non-bacterial infection group (11. 95±4. 58)μg/L (t =2.291,P<0.05);The APACHE Ⅲ score of bacterial infection group(62. 44 ± 19. 55) cent was significantly higher than non-bacterial infection group(44. 56 ± 25. 88) cent(t = 2. 195 ,P < 0. 05). The concentration of PCT of 1.0μg/L and 2. 0 p.g/L compared to the former sensitivity (96. 5) % higher than the latter (55.2) % (X2 = 3. 94, P < 0. 05), the former specificity (41.7) % lower than the latter (95.8)% (X2 = 4. 02 ,P < 0. 05);1.5μg/L as a positive standard Youden index and the Agreement (83.0 % ,0. 65), were significantly higher than 1 μg/L and 2 μg/L(71.7%, 0. 38 ;73.6% ,0. 51) (X2 = 3.84, X2=3. 90,X2 = 3.992 = 3.91 ,P < 0. 05);The concentration of PCT in death group (38. 9 ±12. 6)μg/L was signifi-canfly higher than the survival group(11.8± 8. 3) μg/L(t =2. 398 ,P <0. 05). Conclusion Serum PCT has clinical values in the diagnosis and therapy of bacterial infections in the critical patients.     

危重症患者血清降钙素原测定对细菌感染的诊断价值

Objective To evaluate the clinical value of serum procalcitonin (PCT) in the diagnosis of bacte-rial infections in the critically ill patients. Methods A loud d 100 cases of critical patients were divided into bacteri-al infection group(56 cases) and non-bacterial infection group(44 cases). Serum PCT was measured by immunolu-minometric assay. Results The concentration of PCT in bacterial infection group(21.54 ±5.72) μg/L, was signifi-cantly higher than non-bacterial infection group (11. 95±4. 58)μg/L (t =2.291,P<0.05);The APACHE Ⅲ score of bacterial infection group(62. 44 ± 19. 55) cent was significantly higher than non-bacterial infection group(44. 56 ± 25. 88) cent(t = 2. 195 ,P < 0. 05). The concentration of PCT of 1.0μg/L and 2. 0 p.g/L compared to the former sensitivity (96. 5) % higher than the latter (55.2) % (X2 = 3. 94, P < 0. 05), the former specificity (41.7) % lower than the latter (95.8)% (X2 = 4. 02 ,P < 0. 05);1.5μg/L as a positive standard Youden index and the Agreement (83.0 % ,0. 65), were significantly higher than 1 μg/L and 2 μg/L(71.7%, 0. 38 ;73.6% ,0. 51) (X2 = 3.84, X2=3. 90,X2 = 3.992 = 3.91 ,P < 0. 05);The concentration of PCT in death group (38. 9 ±12. 6)μg/L was signifi-canfly higher than the survival group(11.8± 8. 3) μg/L(t =2. 398 ,P <0. 05). Conclusion Serum PCT has clinical values in the diagnosis and therapy of bacterial infections in the critical patients.     

危重症患者血清降钙素原测定对细菌感染的诊断价值

Objective To evaluate the clinical value of serum procalcitonin (PCT) in the diagnosis of bacte-rial infections in the critically ill patients. Methods A loud d 100 cases of critical patients were divided into bacteri-al infection group(56 cases) and non-bacterial infection group(44 cases). Serum PCT was measured by immunolu-minometric assay. Results The concentration of PCT in bacterial infection group(21.54 ±5.72) μg/L, was signifi-cantly higher than non-bacterial infection group (11. 95±4. 58)μg/L (t =2.291,P<0.05);The APACHE Ⅲ score of bacterial infection group(62. 44 ± 19. 55) cent was significantly higher than non-bacterial infection group(44. 56 ± 25. 88) cent(t = 2. 195 ,P < 0. 05). The concentration of PCT of 1.0μg/L and 2. 0 p.g/L compared to the former sensitivity (96. 5) % higher than the latter (55.2) % (X2 = 3. 94, P < 0. 05), the former specificity (41.7) % lower than the latter (95.8)% (X2 = 4. 02 ,P < 0. 05);1.5μg/L as a positive standard Youden index and the Agreement (83.0 % ,0. 65), were significantly higher than 1 μg/L and 2 μg/L(71.7%, 0. 38 ;73.6% ,0. 51) (X2 = 3.84, X2=3. 90,X2 = 3.992 = 3.91 ,P < 0. 05);The concentration of PCT in death group (38. 9 ±12. 6)μg/L was signifi-canfly higher than the survival group(11.8± 8. 3) μg/L(t =2. 398 ,P <0. 05). Conclusion Serum PCT has clinical values in the diagnosis and therapy of bacterial infections in the critical patients.     

危重症患者血清降钙素原测定对细菌感染的诊断价值

Objective To evaluate the clinical value of serum procalcitonin (PCT) in the diagnosis of bacte-rial infections in the critically ill patients. Methods A loud d 100 cases of critical patients were divided into bacteri-al infection group(56 cases) and non-bacterial infection group(44 cases). Serum PCT was measured by immunolu-minometric assay. Results The concentration of PCT in bacterial infection group(21.54 ±5.72) μg/L, was signifi-cantly higher than non-bacterial infection group (11. 95±4. 58)μg/L (t =2.291,P<0.05);The APACHE Ⅲ score of bacterial infection group(62. 44 ± 19. 55) cent was significantly higher than non-bacterial infection group(44. 56 ± 25. 88) cent(t = 2. 195 ,P < 0. 05). The concentration of PCT of 1.0μg/L and 2. 0 p.g/L compared to the former sensitivity (96. 5) % higher than the latter (55.2) % (X2 = 3. 94, P < 0. 05), the former specificity (41.7) % lower than the latter (95.8)% (X2 = 4. 02 ,P < 0. 05);1.5μg/L as a positive standard Youden index and the Agreement (83.0 % ,0. 65), were significantly higher than 1 μg/L and 2 μg/L(71.7%, 0. 38 ;73.6% ,0. 51) (X2 = 3.84, X2=3. 90,X2 = 3.992 = 3.91 ,P < 0. 05);The concentration of PCT in death group (38. 9 ±12. 6)μg/L was signifi-canfly higher than the survival group(11.8± 8. 3) μg/L(t =2. 398 ,P <0. 05). Conclusion Serum PCT has clinical values in the diagnosis and therapy of bacterial infections in the critical patients.     

危重症患者血清降钙素原测定对细菌感染的诊断价值

Objective To evaluate the clinical value of serum procalcitonin (PCT) in the diagnosis of bacte-rial infections in the critically ill patients. Methods A loud d 100 cases of critical patients were divided into bacteri-al infection group(56 cases) and non-bacterial infection group(44 cases). Serum PCT was measured by immunolu-minometric assay. Results The concentration of PCT in bacterial infection group(21.54 ±5.72) μg/L, was signifi-cantly higher than non-bacterial infection group (11. 95±4. 58)μg/L (t =2.291,P<0.05);The APACHE Ⅲ score of bacterial infection group(62. 44 ± 19. 55) cent was significantly higher than non-bacterial infection group(44. 56 ± 25. 88) cent(t = 2. 195 ,P < 0. 05). The concentration of PCT of 1.0μg/L and 2. 0 p.g/L compared to the former sensitivity (96. 5) % higher than the latter (55.2) % (X2 = 3. 94, P < 0. 05), the former specificity (41.7) % lower than the latter (95.8)% (X2 = 4. 02 ,P < 0. 05);1.5μg/L as a positive standard Youden index and the Agreement (83.0 % ,0. 65), were significantly higher than 1 μg/L and 2 μg/L(71.7%, 0. 38 ;73.6% ,0. 51) (X2 = 3.84, X2=3. 90,X2 = 3.992 = 3.91 ,P < 0. 05);The concentration of PCT in death group (38. 9 ±12. 6)μg/L was signifi-canfly higher than the survival group(11.8± 8. 3) μg/L(t =2. 398 ,P <0. 05). Conclusion Serum PCT has clinical values in the diagnosis and therapy of bacterial infections in the critical patients.     

危重症患者血清降钙素原测定对细菌感染的诊断价值

Objective To evaluate the clinical value of serum procalcitonin (PCT) in the diagnosis of bacte-rial infections in the critically ill patients. Methods A loud d 100 cases of critical patients were divided into bacteri-al infection group(56 cases) and non-bacterial infection group(44 cases). Serum PCT was measured by immunolu-minometric assay. Results The concentration of PCT in bacterial infection group(21.54 ±5.72) μg/L, was signifi-cantly higher than non-bacterial infection group (11. 95±4. 58)μg/L (t =2.291,P<0.05);The APACHE Ⅲ score of bacterial infection group(62. 44 ± 19. 55) cent was significantly higher than non-bacterial infection group(44. 56 ± 25. 88) cent(t = 2. 195 ,P < 0. 05). The concentration of PCT of 1.0μg/L and 2. 0 p.g/L compared to the former sensitivity (96. 5) % higher than the latter (55.2) % (X2 = 3. 94, P < 0. 05), the former specificity (41.7) % lower than the latter (95.8)% (X2 = 4. 02 ,P < 0. 05);1.5μg/L as a positive standard Youden index and the Agreement (83.0 % ,0. 65), were significantly higher than 1 μg/L and 2 μg/L(71.7%, 0. 38 ;73.6% ,0. 51) (X2 = 3.84, X2=3. 90,X2 = 3.992 = 3.91 ,P < 0. 05);The concentration of PCT in death group (38. 9 ±12. 6)μg/L was signifi-canfly higher than the survival group(11.8± 8. 3) μg/L(t =2. 398 ,P <0. 05). Conclusion Serum PCT has clinical values in the diagnosis and therapy of bacterial infections in the critical patients.     

危重症患者血清降钙素原测定对细菌感染的诊断价值

Objective To evaluate the clinical value of serum procalcitonin (PCT) in the diagnosis of bacte-rial infections in the critically ill patients. Methods A loud d 100 cases of critical patients were divided into bacteri-al infection group(56 cases) and non-bacterial infection group(44 cases). Serum PCT was measured by immunolu-minometric assay. Results The concentration of PCT in bacterial infection group(21.54 ±5.72) μg/L, was signifi-cantly higher than non-bacterial infection group (11. 95±4. 58)μg/L (t =2.291,P<0.05);The APACHE Ⅲ score of bacterial infection group(62. 44 ± 19. 55) cent was significantly higher than non-bacterial infection group(44. 56 ± 25. 88) cent(t = 2. 195 ,P < 0. 05). The concentration of PCT of 1.0μg/L and 2. 0 p.g/L compared to the former sensitivity (96. 5) % higher than the latter (55.2) % (X2 = 3. 94, P < 0. 05), the former specificity (41.7) % lower than the latter (95.8)% (X2 = 4. 02 ,P < 0. 05);1.5μg/L as a positive standard Youden index and the Agreement (83.0 % ,0. 65), were significantly higher than 1 μg/L and 2 μg/L(71.7%, 0. 38 ;73.6% ,0. 51) (X2 = 3.84, X2=3. 90,X2 = 3.992 = 3.91 ,P < 0. 05);The concentration of PCT in death group (38. 9 ±12. 6)μg/L was signifi-canfly higher than the survival group(11.8± 8. 3) μg/L(t =2. 398 ,P <0. 05). Conclusion Serum PCT has clinical values in the diagnosis and therapy of bacterial infections in the critical patients.     

他克莫司与西罗莫司根据细胞周期序贯用药对阿霉素肾病大鼠的影响

目的 观察他克莫司、西罗莫司根据细胞周期序贯联合用药对阿霉素肾病大鼠的治疗效果.方法 将24只雄性SD大鼠模型完全随即分为正常对照组、肾病组及治疗组,各8只;测定3组大鼠的尿蛋白定量、血生化指标;免疫组化、定时定量聚合酶链反应检测肾组织Nephrin、Podocin表达以及肾组织结缔组织生长因子(CTGF)的表达.结果 ①肾病组在12周末可见肾小球出现局灶节段性硬化,治疗组病理变化明显减轻.②肾病组4、8、12周末尿蛋白定量[分别为(276.41±11.12)、(355.76±14.10)、(338.92 ±15.87)mg]均明显高于对照组[分别为(12.18±1.38)、(12.98 ±2.07)、(13.56±2.70)mg,P<0.01)],治疗组大鼠尿蛋白定量在8、12周末[分别为(160.64±13.72)、(126.30±14.65)mg]均明显低于肾病组(P<0.05),但高于对照组;肾病组TP、ALB明显低于对照组(P<0.01),肾病组TG、胆固醇明显高于对照组(P<0.01),治疗组TP、ALB明显高于肾病组(P<0.05).③在12周末肾病组大鼠Nephrin、Podocin蛋白和mRNA表达明显减少(P<0.01),治疗组表达较肾病组上调(P<0.05).④在12周末肾病组CTGF蛋白表达较对照组明显增加(P<0.01),治疗组较肾病组明显减少(P<0.05).结论 他克莫司、西罗莫司根据细胞周期序贯联合用药对阿霉素肾病大鼠肾组织病理学变化均有明显改善,可有效减少阿霉素肾病大鼠的尿蛋白;升高TP和ALB,降低TG和胆固醇;并可恢复足细胞相关蛋白的表达,改善肾脏纤维化.

Abstract：

Objective To assess the therapeutic effects of the sequential thempy regimens according to the theory of cell cycle in adriamycin-induced nephrooathy(AIN)rats.Methods AIN model rats were randomly divided into three groups:control group(n=8),AIN model group(n=8)and treated group(n=8).The level of24hour urinary protein,serum total protein(TP),albumin(ALB),cholesterin(Chol),triglyeride(TG),serum urea nitrogen(BUN),serum creatinine(Scr)were measured.The expression of Nephrin and Podocin were analyzed by 11.12),(355.76±14.10)and(338.92±15.87)mg]were obviously higher than control group[respectively (12.18±1.38),(12.98±2.07)and(13.56±2.70)mg].The level of24 hour urinary protein in model group at 8th and 12 th week[respectively(160.64±13.72)and(126.30±14.65)mg]was obviously lower than model group(P<0.05).The levels of TP and ALB in treatment group were significantly higher in AIN model group,and westemblot indicated the expression of CTGF in treatment group was higher than that in AIN model group(P<0.05).Conclusions The sequential combined regimens can improve the pathological changes in Adriamycin-in-duced nephropathy rats,decrease the excretion of urinary protein,increase the levels of TP and ALB and decrease the levels of TG and Chol.It also can increase the expression of Nephrin and Podocin,and ameliorate kidney fibrosis.     

含枳术宽中胶囊的四联疗法治疗单纯幽门螺杆菌感染性胃炎的临床回顾性研究

目的观察含枳术宽中胶囊的四联疗法治疗单纯幽门螺杆菌(Hp)感染性胃炎的疗效。方法治疗组65例,以含枳术宽中胶囊的四联疗法(枳术宽中胶囊+阿莫西林+泮托拉唑+胶体果胶铋)治疗;对照组59例,以四联疗法(阿莫西林+克拉霉素+泮托拉唑+胶体果胶铋)治疗。疗程为2周,分别观察Hp根除率和半年后复发率。结果治疗组和对照组的总有效率分别为89.23%和86.44%,两组比较差异无统计学意义(P>0.05);半年后复发率分别为12.5%和30.56%,两组比较差异有统计学意义(P<0.05)。结论含枳术宽中胶囊的四联疗法治疗单纯幽门螺杆菌感染性胃炎具有良好的疗效,可有效降低Hp根除率,并减少复发。     

中药漱口水联合含氟涂料防治儿童牙龋病的效果及对口腔Hp感染的影响

目的 观察中药漱口水联合含氟涂料防治儿童牙龋病的效果及对口腔Hp感染的影响.结果 选取2013年2月-2014年8月石家庄市中心城辖区6所幼儿园共420名儿童,按防治方法分为对照组和观察组,每组210例,对照组接受局部含氟涂料预防治疗,观察组在对照组基础上加用中药漱口水,涂氟后第2天开始口含中药漱口水.比较2组儿童患龋率、乳牙龋面均(DMFS)、显著性龋均指数(SIC)及防治前后口腔Hp变化情况.结果 防治前2组患龋率、DMFS、SIC和口腔Hp感染率比较差异无统计学意义(P＞0.05).防治后1年和2年,2组患龋率、DMFS、SIC和口腔Hp感染率较防治前上升,但观察组低于对照组,差异有统计学意义(P＜0.05);2组防治后SIC比较差异无统计学意义(P＞0.05).2组不良反应发生率比较差异无统计学意义(P＞0.05).结论 中药漱口水联合含氟涂料防治儿童牙龋病的效果及对口腔Hp感染的改善要优于单独使用含氟涂料,含氟涂料最佳防龋效果还需进一步研究.     

自身免疫性胃炎根除幽门螺杆菌后的相关研究

目的探讨自身免疫性胃炎根除幽门螺杆菌(Hp)后对血清壁细胞抗体(PCA)、血清胃泌素、血清维生素B12、胃内pH值的影响。方法 104例患者均经胃镜及血清壁细胞抗体(PCA)诊断为自身免疫性胃炎(AIG),Hp感染由13C尿素呼气试验或快速尿素酶试验检查证实。患者随机分为两组:治疗组54例,对照组50例。治疗组采用经典根除幽门螺杆菌方案,共服药10d。治疗结束后4～8周,检测Hp根除情况。对照组不用任何对Hp有影响的药物,且与治疗组在同一时间作Hp检测、胃镜及PCA、血清胃泌素、血清维生素B12等检查。结果治疗组Hp根除率为83.8%,对照组根除率为0%(χ2=76.2,P<0.01),在患者根除Hp后PCA阳性率下降,转阴率为72.2%,对照组PCA转阴率为0%(χ2=57.3,P<0.01),具有统计学意义。治疗组治疗与对照组比较自身免疫性胃炎根除Hp后,PCA阳性率明显下降,血清胃泌素降低,胃内pH值及血清维生素B12升高(P<0.01),均具有统计学意义。治疗组上腹部胀痛症状改善明显,对照组中原有上腹部胀痛症状无改善,治疗组贫血明显改善,对照组贫血改善不明显。结论自身免疫性胃炎根除Hp后,PCA阳性率下降,血清胃泌素降低,胃内pH值及血清维生素B12升高。对于Hp感染的自身免疫性胃炎建议根除Hp。     

含左氧氟沙星三联疗法治疗幽门螺杆菌感染相关性消化性溃疡的临床观察

目的探讨含左氧氟沙星的三联疗法在幽门螺杆菌感染相关性消化性溃疡中的疗效。方法 116例幽门螺杆菌感染相关性消化性溃疡患者随机分两组各58例,观察组予左氧氟沙星+泮托拉唑+阿莫西林治疗,对照组予甲硝唑+泮托拉唑+阿莫西林治疗,对比两组疗效与不良反应。结果观察组幽门螺杆菌根除率及总有效率显著高于对照组(P<0.01);两组不良反应发生率差异无统计学意义(P>0.05)。结论含左氧氟沙星的三联疗法在幽门螺杆菌感染相关性消化性溃疡中疗效确切,值得临床推广应用。     

不同尿素呼气试验值阴转后胃食管反流病的治疗随访

目的　观察幽门螺杆菌不同尿素呼气试验 ( UBT)值阴转后在胃食管反流病人中对抑酸剂的影响。方法　症状积分法≥ 6分为反流阳性。1 4C- U BT值≥ 10 0 dm p/ m mol CO2 诊断幽门螺杆菌阳性。观察组按不同 1 4C- UBT阳性值分为 3个组 ,对照组 1 4C- UBT阴性。排除胃、十二指肠溃疡、手术、Barrett′s食管和食管裂孔疝。随访 :每 4周 1次 ,观察奥美拉唑( om eprazole)改为尼扎替定 ( nizatidine)后效果 ,共随访 5次。结果　改服尼扎替定后 UBT值最高组阴转后症状积分降低和有效率显著高于 U BT低值组和对照组 ( P<0 .0 5 )。结论　胃食管反流病合并 U BT高值组 ( >3 0 0 dpm / m mol CO2 )阴转后可以降低用药强度 ;效果在 8周后逐渐增加     

抗幽门螺杆菌治疗腹型过敏性紫癜效果观察

目的:探讨抗幽门螺杆菌(HP)治疗腹型过敏性紫癜(HSP)效果.方法:纳入60例腹型HSP患儿,应用14C尿素呼气试验检测HP,将HP( )患儿随机分为2组:对照组18例,给予抗过敏、抗炎等常规治疗;观察组24例,在常规治疗基础上应用抗HP三联疗法.疗程均为2周,随访时间6个月.比较两组临床疗效及复发率.结果:①腹型HSP HP感染率70%;②观察组临床疗效明显高于对照组(91.67%和61.11%,P＜0.05);复发率显著低于对照组(22.73%和81.82%,P＜0.05).结论:①腹型HSP与HP感染有关;②抗HP三联疗法可提高腹型HSP临床疗效,降低复发率.     

老年人胃黄色瘤与幽门螺杆菌感染的相关性分析

目的 了解老年慢性胃炎患者黄色瘤与幽门螺杆菌(Hp)感染之间的相关关系.方法 对2010年1月至2011年12月在该院接受胃镜检查的60岁以上的慢性胃炎患者499例根据病理结果分为黄色瘤组186例和非黄色瘤组313例,分别观察两组患者胃黏膜Hp感染、中性粒细胞浸润、腺体萎缩、淋巴滤泡等病理表现.结果 黄色瘤组与非黄色瘤组比较,Hp感染率(52.7％、37.3％)、中性粒细胞浸润率(35.5％、24.0％)、腺体萎缩率(36.6％、3.5％)均明显升高(x210.677、7.641、95.606,P＜0.01)；两组淋巴滤泡检出率(21.0％、20.8％)比较,差异无统计学意义(x2=0.003,P=0.957).Hp 感染者中性粒细胞浸润(45.8％)、淋巴滤泡检出率(42.6％)均高于Hp阴性者(14.8％、4.2％),差异均有统计学意义(P=0.000).结论 胃黄色瘤与Hp感染、中性粒细胞浸润、腺体萎缩相关,淋巴滤泡形成与Hp感染密切相关,应加强对老年慢性胃炎患者Hp的根治.     

胃苏颗粒联合奥美拉唑治疗活动期胃溃疡的临床研究

目的比较胃苏颗粒联用奥美拉唑治疗成人活动期胃溃疡患者的临床症状改善情况、内镜下溃疡愈合率以及组织学溃疡愈合质量、生命质量改善程度。方法采用随机对照研究,将92例经胃镜证实伴幽门螺杆菌感染的成人活动期胃溃疡患者,随机分为试验组(47例)和对照组(45例)。治疗第1~2周,2组均予幽门螺杆菌根除四联疗法,试验组同时给予胃苏颗粒5 g·次?1,3次·d?1,泡服。第3~8周,试验组给予胃苏颗粒(剂量服法同前)联合奥美拉唑胶囊,每次20 mg,1次·d?1,口服。对照组仅给予奥美拉唑胶囊20 mg·次?1,1次·d?1,口服。结果治疗第2周末,试验组改善腹痛、腹胀、反酸、嗳气、烧心的发生频率显著优于对照组(P<0.01),试验组改善腹痛程度亦显著优于对照组(P<0.01)。治疗8周后,2组内镜下溃疡愈合总有效率差异无统计学意义。病理组织学检查显示试验组腺体密度和腺管形态恢复显著优于对照组(P<0.05)。治疗后试验组与对照组的生命质量改善程度比较,在心理功能、社会功能的变化上差异均有统计学意义(P<0.01),在生理功能变化上差异无统计学意义。结论治疗活动期胃溃疡,在质子泵抑制剂有效抑酸基础上,联合应用中成药胃苏颗粒,能更快缓解多种临床症状,能显著促进胃黏膜组织形态结构的恢复,提高溃疡愈合质量,同时改善心理功能、社会功能。     

乌灵胶囊治疗伴有焦虑状态的胃食管反流病的疗效

目的：探讨乌灵胶囊治疗伴焦虑状态的胃食管反流病（GERD）患者的临床疗效。方法：选择Zung焦虑自评量表（SAS）评分≥50分的64例GERD患者,随机分为2组,治疗组32例给予乌灵胶囊和埃索美拉唑镁肠溶片,对照组32例给予埃索关拉唑镁肠溶片;疗程8周。应用胃食管反流病症状分级及sAS的评分变化进行疗效评定。结果：治疗8周末,治疗组中显效率、总有效率分别为58．9％和93．1％,对照组分别为32．2％和67．9％,有统计学差异。两组内治疗前与治疗后SAS评分均有统计学差异;治疗后第4、8周末治疗组SAS评分与对照组相比有统计学差异。结论：乌灵胶囊联用埃索美拉唑镁肠溶片能安全有效治疗伴有焦虑状态的GERD患者。     

幽门螺杆菌通过NF-κB信号通路调控胃上皮细胞自噬及凋亡的机制研究

目的 研究幽门螺杆菌通过NF-κB信号通路调控胃上皮细胞自噬及凋亡的机制.方法 体外培养幽门螺杆菌(Helicobacter pylori,Hp)及人胃上皮AGS细胞株,收集Hp处理0 h、3 h、6 h、9 h及对照组、18μmol·L-1 NF-κB特异性抑制剂SN50处理6 h、18μmol·L-1 SN50+Hp处理6 h的AGS细胞,利用流式细胞仪分别检测各组细胞的自噬率及凋亡率;收集Hp梯度时间处理后的AGS细胞,提取各组总蛋白,蛋白质印迹法(Western Blot)检测自噬相关蛋白Beclin1、微管相关蛋白1轻链3Ⅰ(LC3Ⅰ)、微管相关蛋白1轻链3Ⅱ(LC3Ⅱ),凋亡相关蛋白B细胞淋巴瘤/白血病-2(Bcl-2)、Bcl-2相关X蛋白(Bax)及NF-κB通路相关蛋白p65、p-p65的表达.结果 AGS细胞的自噬率与凋亡率均随着Hp作用时间的延长逐渐升高,各时间点与0 h相比均差异有统计学意义(P<0.01).自噬相关蛋白Beclin1、LC3Ⅱ、凋亡相关蛋白Bax及NF-κB通路相关蛋白p-p65的表达随着Hp作用时间的延长逐渐增高,自噬相关蛋白LC3Ⅰ及凋亡相关蛋白Bcl-2蛋白表达随着Hp作用时间的延长逐渐降低,与0 h相比,各检测时间点蛋白表达差异有统计学意义(P<0.01).NF-κB通路相关蛋白p65的表达未出现显著性变化(P>0.05).与对照组相比[(6.56±1.40)%、(11.76±1.70)%],Hp处理后,AGS细胞自噬率(9.86±1.78)%与凋亡率(13.25±1.56)%显著增强(t1=11.054,P1=0.000;t2=10.456,P2=0.000);SN50处理后,AGS细胞自噬率(19.65±2.14)%与凋亡率(28.65±2.14)%显著减弱(t1=3.403,P1=0.009;t2=2.799,P2=0.023);Hp与SN50联合处理后,AGS细胞自噬率(20.65±2.14)%与凋亡率(29.65±2.14)%显著高于对照组,但低于Hp单独处理组,差异有统计学意义(t1=5.164,P1=0.001;t2=5.890,P2=0.000;t3=4.718,P3=0.002;t4=5.738,P4=0.000).结论 Hp刺激显著促进AGS细胞的自噬与凋亡,作用机制可能与NF-κB通路的活化有关.