Expression of Bmi-1,P16,and CD44v6 in Uterine Cervical Carcinoma and Its Clinical Significance

Objective Bmi-1,a putative proto-oncogene,is a core member of the polycomb gene family,which is expressed in many human tumors.The pl6 protein negatively regulated cell proliferation,whereas CD44v6 is associated with proliferation as an important protein.Additionally,CD44v6 is an important nuclear antigen closely correlated to tumor metastasis.The present study aims to investigate the expression and significance of Bmi-1,pl6,and CD44v6 in uterine cervical carcinoma(UCC). Methods A total of 62 UCC,30 cervical neoplasic,and 20 normal cervical mucosal tissues were used in the current study.The expression of Bmi-1,pl6,and CD44v6 in these tissues was determined using immunohistochemical assay.The relationships among the expression of these indices,the clinicopathologic features of UCC,and the survival rate of UCC patients were also discussed.The correlation between Bmi-1 protein expression and pl6 or CD44v6 protein in UCC was analyzed. Results The expression of Bmi-1,p16,and CD44v6 was significantly high in cervical carcinoma compared with that in the cervical neoplasia and normal colorectal mucosa(P<0.05).The over-expression of Bmi-1 protein in UCC was apparently related to the distant metastasis(P<0.01) and the tumor,nodes and metastasis-classification,i.e.the TNM staging,World Health Organization(P<0.05). Nevertheless,the positive expression of pl6 protein in UCC was not significantly associated with the clinicopathologic features (P>0.05).The Kaplan-Meier survival analysis showed that the over-expression of Bmi-1 significantly decreased the survival rate of UCC patients(P<0.05).A strong correlation indicated that there was statistical significance between the expression of Bmi-1 and CD44V6 proteins in UCC(r=0.419,P<0.001). Conclusions The over-expression of Bmi-1 and CD44v6 protein closely correlate to the tumorigenesis,metastasis,and prognosis of UCC.Bmi-1 and CD44v6 may be used to predict the prognosis of cervical carcinoma.Bmi-1 may indirectly regulate the expression of CD44v6 in UCC patients.The positive expression of p16 protein is possibly associated with the tumorigenesis,but not with the metastasis or prognosis of UCC.     

Expression of the EphA2 Gene in Esophageal Carcinoma Tissues

OBJECTIVE To investigate the relationship of the EphA2 gene with the occurrence, invasion and metastasis of esophageal carcinoma. METHODS The expression of EphA2 mRNA was detected by RT-PCR and the EphA2 protein was estimated by immunohistochemistry (SP method) in both esophageal cancerous tissues and normal epithelial tissues. RESULTS The expression of EphA2 mRNA showed no difference between esophageal cancerous tissues and normal epithelium, and there appeared to be no correlation with differentiation of the cancerous tissues, the depth of infiltration or lymph node metastasis (P>0.05). However, the expression of the EphA2 protein was significantly higher in cancerous tissues compared to normal epithelial tissues (P<0.05). The expression of the EphA2 protein in a deeper invasive group and in a group with lymph node metastasis was significantly higher compared to a superficially invasive group and a group without lymph node metastasis (P<0.05). Its expression did not appear to be correlated with differentiation of cancerous tissues (P>0.05). CONCLUSION The occurrence of esophagus carcinoma and the formation of invasion and metastasis may be related to overexpression of the EphA2 protein but not to the level of mRNA, a finding which may due to up-regulation at the translation level or by increased protein stability.     

RhoB-mRNA在喉癌中的表达及其临床意义

Objective To study the expression of RhoB-mRNA,and periphery regions in human laryngeal carcinoma.Methods The expression of RhoB-mRNA were examined by RT-PCR method.Results It was noted that the expression of RhoB-mRNA was positively correlated well with the clinical stages.The expression of RhoB-mRNA in carcinoma with node metastasis group was significantly higher than that without node metastasis.The difference between the two groups was statistically significant(P＜0.05).The expression of RhoB-mRNA in different types and stages of laryngeal carcinoma was significantly different(P＜0.05).The expression of RhoB in laryngeal carcinoma negtive correlation with the stages of laryngeal carcinoma(r=0.557).Conclusion The expression of RhoB plays an inhibitive role in the growth,invasion and local node metastasis of human laryngeal carcinoma.RhoB promote the formation,invasion and lymph node metastasis as oncogenes.It may be valuable as predictors for lymph node metastasis and prognosis.     

Expresston of the EphA2 Gene in Esophageal Carcinoma Tissues

OBJECTIVE To investigate the relationship of the EphA2 gene with the occurrence, invasion and metastasis of esophageal carcinoma. METHODS The expression of EphA2 mRNA was detected by RT-PCR and the EphA2 protein was estimated by immunohistochemistry （SP method） in both esophageal, cancerous tissues and normal epithelial tissues. RESULTS The expression of EphA2 mRNA showed no difference between esophageal cancerous tissues and normal epithelium, and there appeared to be no correlation with differentiation of the cancerous tissues, the depth of infiltration or lymph node metastasis （P〉0.05）. However, the expression of the EphA2 protein was significantly higher in cancerous tissues compared to normal epithelial tissues （P〈0.05）. The expression of the EphA2 protein in a deeper invasive group and in a group with lymph node metastasis was significantly higher compared to a superficial： ly invasive group and a group without lymph node metastasis （P〈0.05）. Its expression did not appear to be correlated with differentiation of cancerous tissues （P〉0.05）. CONCLUSION The occurrence of esophagus carcinoma and the formation of invasion and metastasis may be related to overexpression of the EphA2 protein but not to the level of mRNA, a finding which may due to up-regulation at the translation level or by increased protein stability.     

结直肠癌中CDX2、PTEN、Ki-67的表达及DNA倍体分析

Objective To investigate the expression of CDX2,PTEN and Ki-67 in colorectal carcinoma,and the correlations among CDX2,PTEN,Ki-67 and DNA ploidy. Methods Fifty samples of human colorectal carcinoma tissue and ten samples of normal mucosa adjacent to carcinoma were examined for CDX2,PTEN as well as Ki-67 by immunohistochmistry,and the flow cytometry was used to detect the status of DNA ploidy jn colorectal carcinoma.Results The expression of CDX2 in colorectal carcinoma and normal mucosa tissue were 82%(41/50) and 100%(10/10) respectively.The expression of CDX2 had significant difference compared to that in normal mucosa tissue.PTEN in colorectal carcinoma and normal mucosa were 64%(32/50)and 90%(9/10) respectively,the expression had significant difference.Both CDX2 and PTEN were related with staging and grading of tumor,metastasis of lymph nodes and infiltrating depth of carcinoma.Expression of CDX2 and PTEN was negatively correlated with the expression of Ki-67(r=-0.254,P＜0.05;r=-0.340,P＜0.01) respectively.The expression of CDX2 was increased in diploid colorectal cancer,as compared with that in heteroploidy colorectal cancer(93.75%and 76.47%).But the expression of PTEN had no difference between diploidy and heteroploidy carcinomas.Conclusion The expression of the CDX2 and of the PTEN were are positive in part of the colorectal carcinoma.Both CDX2 and PTEN's expression negatively correlate with Ki-67.CDX2,intestinal-specific homeobox genes,regulate cell proliferation and differentiation in the continuously renewed intestinal epitheium.PTEN positively regulates CDX2 gene expression.The down regulation of CDX2 protein is concerned with tumor maligancy and bad prognosis.     

喉癌术前平阳霉素诱导性化疗的临床及基础研究

Objective： To evaluate the short-term effects of neoadjuvant chemotherapy with pingyangmycin （PYM） in the treatment of laryngeal squamous cell carcinoma. Methods： 24 cases of laryngeal squamous cell carcinoma were treated with PYM before the operation, and the surgeries were undergone within one week after neoadjuvant chemotherapy. PCNA, p53, Bcl-2 and CD44v6 were detected in the specimens of tumor, retreated tumor and normal tissue using immunohistochemical methods. Results： Apoptosis could be detected more often in specimens with tumor and retreated tumor after chemotherapy than that before. The expression of PCNA, p53, Bcl-2 and CD44v6 in tumor tissue after neoadjuvant chemotherapy with PYM was weaker than that before the chemotherapy. There was significant difference in the positive ratio of PCNA, p53, Bcl-2 and CD44v6 between retreated tumor and tumor. Conclusion： After neoadjuvant chemotherapy with PYM, a large number of tumor cells died. The amplification and metastasis of tumor were suppressed by neoadjuvant chemotherapy with PYM.     

P73、ER、PR在食管癌及癌前病变中的表达及临床意义

Objective： To test the expression of mutant p73, p53, ER and PR proteins in the esophageal normal mucosa, hyperplasia, dysplasia and squamous cell carcinoma, and research the clinically pathological significance and the correlation, for the early diagnosis, prognostic measure and therapy in clinic. Methods： With Immunohistochemistry, it was examined to show these tumor markers＇ expression in different epithelial lesions of 40 esophageal squamous cell carcinomas, 14 dysplasias, 14 hyperplasias and 14 normal mucosas. Results： The expression of p73 was 55%, 21%, 0% and 0% in the esophageal carcinoma, dysplasia, hyperplasia and normal mucosa, respectively. The significant difference in expression of p73 （P〈0.001） was observed between the esophageal normal mucosia, hyperplasia, dysplasia and esophageal squamous cell carcinoma with Fisher＇s exact test. Difference in expression of p73 （P〈0.05） was observed between the esophageal squamous cell carcinoma and dysplasia with X^2 test. The expression of p73 showed non-correlation with the patient＇s age, sex, tumor＇s grade, lymph-node metastasis and invasive depth （P〉0.05）; Similarly, the expression of mutant p53 was 67.5%, 35.7%, 7% and 0%, respectively; In like manner, the expression of ER was 55%, 21.4%, 14.2% and 0%, respectively; The expression of PR was 57.5%,14.28%, 0% and 0%, respectively. The significant difference in expression of PR （P〈0.001） was observed with Fisher＇s exact test. Difference in expression of PR （P〈0.05） was observed between the esophageal squamous cell carcinoma and dysplasia with x2 test. The expression of PR （P〈0.05） was correlated with lymph-node metastasis, and showed non-correlation with the patient＇s age, sex, tumor＇s grade, and invasive depth （P〉0.05）. Moreover, over-expression of mutant p53 and p73 showed significant correlation with ER and PR protein＇s positive expression （P〈0.05）. Conclusion： P73 protein may become a new tumor＇s marker to diagnose esophageal squarnous celt carcinoma. Because the expression of p73 protein was closely correlated with ER and PR, they could be simultaneously examined to help to early diagnose, prognosticate and cure esophageal carcinoma.     

结直肠癌趋化因子MCP-1的表达及其与巨噬细胞浸润的关系

Objective： To study the expression of MCP-1 in colorectal carcinoma and its relationship to the infiltration of the macrophage and to the biological behaviour of infiltration and metastasis of colorectal carcinoma. Methods： The expression of the MCP-1 mRNA was assessed in colorectal carcinoma collected freshly from surgical specimen by RT-PCR and the expression of the MCP-1 protein was assessed in colorectal carcinoma collected from surgical specimen by immunohistochemistry. The tumor infiltrating cell and macrophage were also investigated by immunohistochemistry. Results： All the 12 specimens of colorectal carcinoma detected by RT-PCR expressed the MCP-1 mRNA; MCP-1 protein was detected in 90% （36/40） cases of the tumor; The expression of the MCP-1 protein in colorectal carcinoma correlated negatively with its state of metastasis and the Dukes＇ stage. But a postive correlation was found between the expression of MCP-1 and the infiltrated macrophage. The stronger expression of MCP-1, the more number of the infiltrated macrophage. Conclusion： The expression of chemokine MCP-1 in colorectal carcinoma may influence its biological behaviour of infiltration and metastasis, and can attract the immuno-cell to the local of the tumor, such as Macrophage.     

CATHEPSIN B EXPRESSION AND ITS RELATIONSHIP WITH MICROVESSEL DENSITY AND BIOLOGICAL BEHAVIOUR OF COLORECTAL CARCINOMA

The proteolytic enzymes secreted by cancer cells are believed to play an important role in cancer invasion and metastasis. Our studies have shown that Cathepsin D is correlated with the invasion and metastasis of colorectal carcinoma. But there are no significant differences between mucinous and non-mucinous colorectal carcinoma for cathepsin D expression. The results suggest that there maybe other closely related factors which make mucinous colorectal carcinoma apt to metastasis compared wit…     

CD44v6、p27表达与大肠癌临床病理学因素的关系

目的探讨CD44v6和p27在大肠癌中的表达及其临床意义。方法对50例手术切除的大肠癌新鲜标本及相应的正常大肠黏膜,应用流式细胞术（FCM）检测CD44v6的表达;同步制备上述病例大肠癌组织石蜡标本,采用免疫组织化学方法检测大肠癌组织中p27的表达,结合病理及临床资料进行统计学分析。结果CD44v6和p27在大肠癌组织及正常黏膜组织中表达存在明显差异（P〈0．01）,而且CD44v6和p27的表达与大肠癌的分化程度、有无淋巴结转移、有无远端器官转移、Duke分期及预后相关（P值均〈0．05）,CD44v6。的表达与大肠癌的浸润深度无明显相关（P〉0．05）,但p27的表达与大肠癌的浸润深度明显相关（P〈0．05）。结论CD44v6和p27的表达与大肠癌的临床病理学特征密切相关,两者在大肠癌组织中的表达呈一定的负相关,同时检测二者对判断大肠癌的恶性程度、复发转移潜能,评价患者的预后及术后选择合理的治疗方案具有一定的参考价值。     

CD44v6、P27蛋白在乳腺癌组织中的表达及意义

目的 探讨乳腺癌中CD44v6、p27蛋白表达的意义。方法 应用免疫组化S-P法检测65例乳腺癌中CD44v6、p27蛋白表达。结果 65例乳腺癌中CD44v6、p27蛋白表达总阳性率分别为58.4％、38.4％。CD44v6蛋白阳性表达率与乳腺癌组织学类型、腋淋巴结转移呈正相关(P<0.05,P<0.01),与预后呈负相关(P<0.01),与组织学分级无明显相关性(P>0.05);p27蛋白阳性表达与乳腺癌组织学类型、组织学分级及腋淋巴结转移呈负相关(P<0.05),与预后呈正相关(P<0.05)。结论 CD44v6、p27蛋白表达与乳腺癌浸润、转移及预后显著相关,检测乳腺癌中CD44v6、p27蛋白表达水平,对判断乳腺癌恶性程度、复发转移潜能,评估患者预后及术后选择合理治疗方案有一定参考价值。     

p27和CD44V6在卵巢癌中的表达及其诊断价值

 目的 探讨p27及CD44V6在卵巢癌中的表达特点及其在判断卵巢癌患者预后中的意义。方法 应用免疫组化（二步法）技术对54例卵巢癌组织进行表达检测，结合临床病理学指标进行分析。结果 p27在卵巢癌的阳性表达率为（20／54）37.0 %，CD44V6的阳性表达率为（23／54）42.6 %。p27在卵巢癌的临床Ⅲ ~ Ⅳ期、低分化肿瘤和淋巴结转移阳性组的表达率分别为20.0 %，12.0 %和8.7 %，显著低于临床Ⅰ~Ⅱ期、高中分化肿瘤和淋巴结转移阴性组的表达率68.4 %，58.6 %和58.1 %，P值分别为＜0.05，＜0.05和＜0.01。CD44V6在卵巢癌的临床Ⅲ~Ⅳ期和淋巴结转移阳性组的表达率分别为57.1 %和73.9 %，显著高于临床Ⅰ~Ⅱ期和淋巴结阴性组的表达率15.8 %和19.4 %，P值分别为＜0.05，＜0.05。结论 p27和CD44V6在卵巢癌的发生、发展中起着重要的调控作用。p27的缺失表达和CD44V6的高表达对卵巢癌的浸润、转移起重要作用，对分析卵巢癌的生物学行为和预后估计有重要意义。     

p27与消化道肿瘤及其应用前景

p27具有多种生物学功能.其表达下降与消化道肿瘤的发生、发展、侵袭密切相关,并可作为独立的预后判定指标.p27及人工修饰构建的p27突变体在肿瘤基因治疗中有着广泛的应用前景.     

胃癌组织MCM7和p27蛋白表达及其临床意义的探讨

目的:探讨微小染色体维持蛋白7(MCM7)和p27蛋白在胃癌组织中的表达及其意叉.方法:采用免疫组化法检测MCM7和p27蛋白在20例正常胃黏膜、30例低级别非典型增生、80例胃癌组织中的表达,并以标记指数(LI)量化及其表达.结果:MCM7和p27蛋白在正常胃黏膜、低级别非典型增生和胃癌组织中的表达之间差异均有统计学意叉(P＜0.05);MCM7的LI在胃癌组织中表达与临床分期、浆膜浸润、术后复发,肝转移及淋巴结转移密切相关(P＜0.05),与肿瘤大小和组织学分级无关(P＞0.05).p27的LI在胃癌组织中表达与临床分期、肝转移和淋巴结转移密切相关(P＜0.05),与浆膜浸润、术后复发,肿瘤大小和组织学分级无关(P＞0.05).胃癌组织中MCM7和p27的LI表达呈显著负相关(γ=-0.313,P=0.005).结论:MCM7的表达升高和p27蛋白的表达下降与胃癌的发生及发展密切相关,检测MCM7和p27有助于判断胃癌恶性程度及预后.     

肝癌及癌前病变中p27蛋白和mRNA的表达

[目的]研究肝细胞癌(HCC)及癌前病变中细胞周期负性调控因子p27的表达及其意义.[方法]应用免疫组织化学S-P染色法和原位分子杂交法检测正常肝组织、慢性肝炎、肝硬化、癌周肝硬化和肝癌组织中p27蛋白及其mRNA的表达,并对结果进行计算机图像定量分析.[结果]p27蛋白在正常肝组织和慢性肝炎中呈低表达,在肝硬化和癌周肝硬化中表达明显增强,而在肝癌中表达明显下降,与前4组比较差异有显著性(P＜0.05).p27mRNA在非肝癌组织中表达均较强,在肝癌中表达明显减弱,与前4组比较差异有显著性(P＜0.05).正常肝组织、慢性肝炎和肝硬化中p27蛋白阳性信号主要定位于胞核,而癌周肝硬化和HCC中主要定位于胞浆.随着HCC的分化程度降低,p27蛋白和p27mRNA的表达减弱,差异有显著性(P＜0.05).[结论]p27表达下降可能参与了HCC的发生,且与HCC的分化有关.p27的胞浆定位可能与HCC的早期发生有关.     

PTEN表达在胃癌发生及分化中的作用

目的:探讨PTEN的表达与胃黏膜癌变及癌细胞分化程度的关系.方法: 采用免疫组织化学方法对胃癌组织、癌前病变和对照组正常胃黏膜进行PTEN表达的检测.结果: 胃癌组PTEN阳性率为53.3%,较正常组和癌前病变组明显下降(P <0.01);低分化组PTEN阳性率为36.0%,显著低于胃癌高中分化组 (P <0.01);高中分化组PTEN阳性率与癌前病变组无差异(P>0.05).结论: PTEN与胃癌的分化程度密切相关,可作为判断胃癌恶性程度的指标.     

p27在50例宫颈癌中的表达及意义

[目的]探讨p27在宫颈癌中的表达及其临床意义。[方法]应用免疫组化法检测50例宫颈癌、25例宫颈上皮内瘤样变（CIN）和10例正常宫颈组织石蜡包埋组织中p27蛋白的表达。[结果]p27在宫颈癌中的阳性表达率为32.00%（16/50）,在CIN中的阳性表达率为56.00%（14/25）,在正常宫颈组织中的阳性表达率为80.00%（8/10）,三者比较差异有统计学意义（P〈0.01）。p27高表达与宫颈癌患者的年龄、临床分期、病理类型、组织学分级均无相关性（P〉0.05）。[结论]p27在宫颈癌低表达,其可作为宫颈癌癌前病变的生物学标志物。     

p27表达水平与非小细胞肺癌预后关系的Meta分析

目的：采用Meta分析的方法，探讨p27在非小细胞肺癌（NSCLC）预后中的作用。方法：检索PubMed、CNKI中研究p27表达与NSCLC预后关系的文献，收集每篇文献的相对危险比（HR）及95％可信区间（95％CI），应用Meta分析Dersimonian—Laird模型对文献进行定量综合分析。结果：共入选7篇文献，累计NSCI。C病例888例，其中p27阳性表达443例，阳性率49．9％。对入选7篇文献进行一致性检验，文献具有异质性（Q=102．13，P=0．000），合并相对危险比为1．21（95％可信区间：1．13～1．29，P=0．000）。结论：p27的阳性表达可能是NSCLC的良好预后因素。