猪体内亚洲牛带绦虫囊尾蚴自然感染的发现

目的证实亚洲牛带绦虫的感染与生食猪肝的关系。方法对贵州都匀流行地区屠杀的猪进行调查。结果首次在猪肝上发现亚洲牛带绦虫自然感染的囊尾蚴,猪的自然感染率为8．87％（11／124）,共检获囊尾蚴17个,感染强度为1～4个。结论亚洲牛带绦虫的感染与当地村民的生活方式、饮食卫生习惯（生食猪肝等内脏）有关。     

Influence and related mechanism of Retn gene expression on glucose uptake in 3T3-L1 cells

The aim of this article was to investigate the influence and the related mechanism of the Retn gene on glucose uptake and insulin resistance in 3T3-L1 cells. Radioimmuno-assay was used to determine glucose uptake in 3T3-L1 cells with different Retn gene expression levels, whether cells were stimulated by insulin or not. RT-PCR and real-time RT-PCR analysis were used to determine the mRNA levels of several glucose transport proteins in 3T3-L1 cells with different Retn gene expression levels, including insulin receptor substrate-1(IRS-1), phosphatidylinositol 3-kinase (PI-3K), AKT-2, glucose transporter-4 (GLUT-4), p38 mitogen-activated protein kinase (p38MAPK) and glycogen synthase kinase-3β (GSK-3β). The glucose uptake decreased with the increase in Retn gene expression in 3T3-L1 cells, which was independent of whether the cells were stimulated by insulin or not. The mRNA expression of two signal proteins PI-3K and AKT-2 decreased and the other two signal proteins, GSK-3β and p38MAPK, increased with Retn overexpression in 3T3-L1 cells. Resistin could induce insulin resistance in adipocytes, which might be related to the changes of some proteins in PI-3K and Ras pathways. Translated from Academic Journal of Second Military Medical University, 2006, 27(10): 1,067–1,071 [译自: 第二军医大学学报] The authors contributed equally to the study.     

Immunohistochemical characterization of hepatic stem cell-related cells in developing human liver

Little is known about the expression characteristics of the various kinds of possible markers in hepatic stem cells (HSCs) and other HSC-related cells in human fetal liver in various developmental stages. It is significant to investigate the immunohistochemical expression for better understanding of the origin, differentiation and migration of HSCs in the developing human liver. H-E staining and immunohistochemical methods were used to observe the expression of hepatic/cholangiocellular differentiation markers (AFP, GST-π, CK7, CK19) and hematopoietic stem cell markers(CD34 and c-kit) in several kinds of HSC-related cells in thirty cases of fetal liver samples (4–35 weeks after pregnancy). AFP expression appears in fetal hepatocytes at four weeks’ gestation. It peaks at 16–24 weeks’ gestation and decreases gradually afterwards. Finally, weak signals were only found in some ductal plate cells and a few limiting plate cells. GST-π was detected in hepatic cord cells from the sixth week and in the ductal plate cells from the eighth week. Twenty-six weeks later, only some ductal plate cells and a few limiting plate cells show positive signals. CK19 expression peaks during the 6th–11th weeks in hepatic cord cells and decreases gradually afterwards, except for the ductal plates. CK7 expression was limited in the ductal plate cells and bile ducts cells from the 14th week. CD34 and c-kit were detected at the eighth week in some ductal plate cells and a few mononuclear cells in the hepatic cords/mesenchymal tissue of portal areas. After 21 weeks, CD34 and c-kit were found only in ductal plate cells and a few mononuclear cells in the hepatic mesenchymal tissue of portal areas. Fetal hepatocytes at 4–16 weeks’ gestation are mainly constituted by HSCs characterized with bi-potential differentiation capacity. At 16 weeks’ gestation, most hepatic cord cells begin to differentiate into hepatocytes and abundant HSCs remain in ductal plate (the origin site of Hering canals). It is also indicated that the hematopoietic stem cells may give rise to some HSCs in embryonic liver. These indirectly support the hypothesis about the location and origin of HSCs in “liver valley hypothesis” reported previously. Translated from Academic Journal of Second Military Medical University, 2007, 28(2): 117–121 [译自: 第二军医大学学报]     

Association study on GNB3 gene polymorphism with essential hypertension in Xinjiang Uygur group

The relationship between the tenth exon C825T of G-protein β3 subunit (GNB₃) genetic polymorphism and hypertension in the Uygur population of China was investigated. A nested case-control study (n = 738) was carried out. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique was used to genotype GNB₃ C825T polymorphism in 354 hypertensive (HT) and 384 normotensive (NT) Uygur subjects. The distributions of GNB₃ C825T genotypes were CC (27.2%), TT (42.9%), and CT (29.9%) in the hypertensive subjects and CC (27.7%), TT (42.4%), CT (29.9%) in the normotensive subjects. There were no significant differences in the genotype distributions between the two groups (χ ² = 0.0262 P = 0.99). The T allele was 51.4% in hypertensive subjects and 51.2% in normotensive subjects, which, between the two groups, was not a significant difference (χ ² = 0.0016 P = 0.97). Further analysis shows that there is no association between C825T genotypes and age, body mass index (BMI), Glucose (GLU), Triglyceride (TG), Cholesterol (CHO), systolic blood pressure (SBP) and diastolic blood pressure (DBP). No evidence was found to suggest an association between GNB₃ C825T polymorphism and hypertension in the Uygur population of China. Translated from Journal of Fudan University (Medical Sciences), 2006, 33(4): 433–436 [译自: 复旦大学学报 (医学版)]     

Genetics analysis of haptoglobin gene in Fujian Han nationality

To study the genetic features (characteristics) of haptoglobin gene, four different age groups of Fujian Han people were investigated. The phenotypes of the haptoglobin of four different groups were analyzed by using polyacrylamide gel electrophoresis. The frequency of HP ¹ in the population of Fujian Han nationality accounted for 0.340, among which children, youths, middle aged and elder groups were 0.307, 0.338, 0.363 and 0.383, respectively. The Hp^0–0 phenotype frequency was 0.026 in which the four age groups accounted for 0.032, 0.046, 0.014 and 0.014, respectively. The frequency of Hp ¹ gene is rising with increasing age. The frequency of Hp^0–0 phenotype is highest in the middle aged group and then tends to drop with increasing age. __________ Translated from Journal of Fujian Medical University, 2007, 41(5): 452–453 [译自: 福建医科大学学报]     

Pathological study of 130 cases of nonalcoholic fatty liver disease based on NASH-CRN system

To summarize the pathological features of nonalcoholic liver disease (NAFLD) in China based on a histological scoring system for NAFLD designed by the Pathology Committee of NASH Clinical Research Network (NASH-CRN), the specimens of liver needle biopsies from 130 patients with NAFLD were histopathologically analyzed by haematoxylin eosin, reticular fiber and Masson trichrome stain. Immunohistochemistry staining was used to exclude non-NAFLD cases combined with clinical data. Hepatic steatosis, lobular inflammation, hepatocytic ballooning and fibrosis were presented widespread in NAFLD liver tissues. Furthermore, macrovesicular steatosis predominantly located in acinar zone 3 was the main histologic feature of NAFLD and lobular inflammation was usually presented mildly. Hepatocyte ballooning was observed in 94.6% of all 130 cases. Mild perisinusoidal fibrosis and periportal fibrosis were often observed in stage 1 cases. According to the statistic analysis, hepatic steatosis was positively correlated with lobular inflammation, hepatocytic ballooning and fibrosis (r = 0.587, 0.488, 0.374, respectively, all P value < 0.01). The number of microgranulomas, lipogranulomas and apoptotic bodies increased following severity of steatosis, lobular inflammation and fibrosis. Meanwhile, the number of megamitochondria and glycogen nuclei was paralleveled to the degree of hepatocytic ballooning (P value all < 0.01). We suggest that the role of portal inflammation should be emphasized besides hepatic steatosis, lobular inflammation, hepatocyte ballooning and fibrosis in diagnosis and evaluation of NAFLD. It needs to be further verified whether microgranulomas, lipogranulomas and apoptosis bodies could be used as histopathological markers of development of NAFLD. The number of megamitochondria is more frequently be found in NAFLD, while in alcoholic liver diseases was Mallory bodies. __________ Translated from Medical Journal of Chinese People’s Liberation Army, 2007, 32(2): 96–99 [译自: 解放军医学杂志]     

Association of polymorphism of TLR4 and CD14 genes with gastroduodenal diseases in Japan

Background: Host genetic factors may play a key role in determining the long-term outcome of the Helicobacter pylori infection. Toll like receptor 4(TLR4) and CD14-mediated recognition of lipo-polysaccharide (LPS) is required for efficient recognition of Gram-negative bacterial infections. Aims: We investigated the effects of common polymorphisms of TLR4 Asp299Gly, Thr399Ile and CD14 promoter –C159T on the risk of gastric cancer including its subtypes and clinicopathologic features. We also investigated the effects of these polymorphisms on histologic degree of H. pylori induced gastritis. Subjects: The study was performed in 149 gastric cancer(GC) cases [mean age 64.0 ±12.4, M:F = 109:40] and 94 patients without evidence of GC (mean age 64.1 ±12.3, M:F = 65:25, Peptic ulcer diseases = 43.6 %, gastritis = 56.4 %) as the control group. Methods: TLR4 Asp299Gly, Thr399Ile and CD14 promoter – C159T were determined by PCR-RFLP in all the patients. Gastritis scores of non-cancerous gastric mucosa were assessed according to the updated Sydney system in H. pylori-positive subjects(n = 174). Results: The frequencies of CD14-260 TT and T carrier were significantly lower in patents with intestinal type gastric cancer than in controls (OR = 0.31;95 % CI = 0.12–0.78, OR = 0.38; 95 % CI = 0.18–0.81, respectively) Compared with patients older than 61 years, the atrophy score in antrum was significantly lower in TT and CT patients.TLR4 Asp299Gly, Thr399Ile were not detected in all the patients. Conclusion: Our data suggest that CD14 promoter-159TT and T carrier were associated with lower risk of developing gastric mucosal atrophy in H. pylori infected patients more than 61 years of age, and these genotypes may reduce the risk of intestinal type gastric cancer and TLR4 Asp299Gly, Thr399Ile are very rare in the Japanese population. Received 12 July 2006; accepted 17 August 2006     

Changes of phenotype and function of human CD4+CD25-T cells induced by transfection of Foxp3

The aim of this paper is to explore the effects of transfection of Foxp3 gene on the phenotype and function of naive CD4⁺ T cells. The pMSCV-Foxp3 retroviral vector encoding Foxp3 gene was transduced into the PT67 packaging cell line. Virus-containing supernatant was applied to differentiate CD4⁺CD25⁻ T cells. The resulting cells were sorted with flow cytometry. The expressions of CD25, CD127, CTLA-4 and the proliferation of transfected T cells were examined. The effect of transfected CD4⁺ T cells on the proliferation and cytokine production of CD4⁺CD25⁻ T cells was examined. Foxp3-gene transfected CD4⁺ T cells could express Foxp3 and transfection of Foxp3 gene up-regulated the expressions of CD25 and CTLA-4, but down-regulated CD127 expression. After transfection, the proliferation of CD4⁺ T cells was eliminated. Transfected T cells inhibited the proliferation of CD4⁺CD25⁻ T cells. CD4⁺CD25⁻ T cells acquired a regulatory phenotype and function after it was transduced with the Foxp3 gene. This suggested a key role of Foxp3 in the generation of CD4⁺CD25⁺ regulatory T cells. __________ Translated from Acta Academiae Medicinae Militaris Tertiae, 2008, 30(3): 186–188 [译自: 第三军医大学学报]     

Effects of podocin transfection on CD2AP distribution in HEK293 cells

The aim of this paper is to construct a podocin fluorescence expression vector and observe the effects of podocin transfection on CD2AP distribution in HEK293 cells. The pGEMT-easy vector containing the full-length cDNA encoding human podocin was cloned and digested with BamHI and XhoI. The digested full-length podocin was subcloned into pEGFP-C2. The constructed plasmids were transfected into HEK293 cells and its effects on CD2AP distribution were observed by immunofluorescence. The pEGFP-NPHS2 expression vector was successfully constructed and podocin exclusively located on the HEK293 cell membrane. After podocin transfection, CD2AP redistributed from the perinucleus to the cytoplasm in HEK293 cells. It can be concluded that podocin can recruit CD2AP to redistribute from the perinucleus to the cytoplasm in HEK293 cells. __________ Translated from Journal of Nanjing Medical University (Natural Science), 2007, 27(6): 527–529, 533 [译自: 南京医科大学学报(自然科学版)]     

Study on the action of resistin-induced human umbilical vein endothelial cell dysfunction

The aim of this paper was to investigate the effects of resistin on human umbilical vein endothelial cells (HUVECs), and to explore its role and mechanism of action in atherosclerosis. HUVECs were incubated with recombinant human resistin (0, 50, 100 ng/mL) for 24 h. ICAM-1, VCAM-1 and reactive oxygen species (ROS) were assayed by flow cytometer. ET-1, eNOS and iNOS mRNA expression were measured by semi-quantitative RT-PCR. Incubation of HUVECs with resistin resulted in an increase in ICAM-1 expression and ET-1 mRNA expression. However, resistin had no effect on VCAM-1 expression and ROS release. eNOS and iNOS mRNA expression were not altered by resistin stimulation. Adipokine resistin exerted a direct effect in promoting HUVEC dysfunction by promoting ICAM-1 and ET-1 expression. These data suggest that adipocyte-endothelium cross-talk might play an important role in the pathogenesis of cardiovascular disease in diabetes mellitus. Translated from Journal of Sun Yat-Sen University (Medical Sciences), 2006, 27(3): 258–261 [译自: 中山大学学报 (医学科学版)]     

Dual therapy with high doses of rabeprazole and amoxicillin versus triple therapy with rabeprazole,amoxicillin, and metronidazole as a rescue regimen for <Emphasis Type="Italic">Helicobacter pylori</Emphasis> infection after the standard triple therapy

Backgrounds and Aims Development of safe and effective rescue regimens for eradication failure of Helicobacter pylori infection by standard regimens is an urgent task. We designed the prospective study to compare the efficacy of two rescue regimens after eradication failure by the standard triple therapy. Methods One hundred and thirty-two patients in whom eradication of H. pylori infection failed initial triple therapy with lansoprazole 30 mg b.i.d, amoxicillin 750 mg b.i.d. and clarithromycin 400 mg b.i.d. for 1 week were randomized to either the 1–week triple therapy with rabeprazole 10 mg b.i.d., amoxicillin 750 mg b.i.d., and metronidazole 250 mg b.i.d. (RAM) or the 2–week dual therapy with rabeprazole 10 mg q.i.d. and amoxicillin 500 mg q.i.d. (RA). Eradication of H. pylori was judged by ¹³C-urea breath test 1 month later. Results The intention-to-treat and per-protocol-based eradication rates were 92.4% (95% CI: 83.2–97.5) and 95.3% (95% CI: 86.9–99.0) for the RAM therapy and 90.9% (95% CI: 81.2–96.6) and 93.8% (95% CI: 84.8–98.3), respectively, for the RA therapy (P > 0.2 for both). No clinically recognizable adverse events were observed with either regimen. Conclusion RA as well as RAM therapy are safe and effective rescue regimens for H. pylori infection after eradication failure by the standard triple therapy.     

柱前衍生化HPLC法测定积雪草及三金片中积雪草苷的含量

目的　建立积雪草中积雪草苷的柱前衍生化高效液相色谱测定法,并用该法测定积雪草和三金片中积雪草苷的含量。方法　以吡啶—苯甲酰氯为衍生化试剂,对积雪草苷分子中的羟基进行苯甲酰化后,采用RP-HPLC法测定。色谱柱：Spherisorb ODS C₁₈柱;流动相：甲醇—四氢呋喃—水(90∶4∶6,0.1%三乙胺);柱温：25℃;流速：0.8 ml.min^-1;检测波长：235 nm;内标物：V_D3。结果　积雪草苷0.396～3.960 μg与峰面积比呈线性关系,相关系数为0.9985。用此法对不同产地的积雪草和三金片中积雪草苷进行测定,结果积雪草中积雪草苷的平均回收率为99.6%,RSD为2.3%(n=6);三金片中积雪草苷的平均回收率为99.2%,RSD为2.7%(n=6)。结论　此法准确、灵敏度高、重现性好,为中药积雪草和中成药三金片的质量评价提供了科学依据。     

Visualization of vascular ultrastructure during osteogenesis by tissue engineering technique

The aim of this paper was to observe and visualize the changes in osteoblasts by electron microscopy during osteogenesis using tissue engineering technique. We also studied the feasibility of improving tissue vascularization of the engineered bone by using small intestine submucosa (SIS) as the scaffold. Bone mesenchymal stem cells (BMSCs) were isolated by gradient centrifugation method. Bone mesenchymal stem cells were seeded in the SIS, and the scaffold-cell constructs were cultured in vitro for 2 weeks. Small intestine submucosa without BMSCs served as control. Both SIS scaffolds were then implanted subcutaneously in the dorsa of athymic mice. The implants were harvested after in vivo incubation for 4, 8 and 12 weeks. The changes in osteoblasts and vascularization were observed under a transmission electron microscope and a scanning electron microscope. The BMSCs grew quite well, differentiating on the surface of the SIS and secreting a great deal of extracellular matrices. The scaffold-cell constructs formed a lot of bone and blood vessels in vivo. The scaffold degraded after 12 weeks. No osteoblasts, but vascularization and fibroblasts were observed, in the control. The SIS can be used as a scaffold for constructing tissue-engineered bone as it can improve the formation of bone and vessels in vivo. Translated from Journal of Shanghai Jiaotong University (Medical Science), 2006, 26(2): 113–116 [译自: 上海交通大学学报 (医学版)]     

Tranilast ameliorates impaired hepatic functions in Schistosoma mansoni-infected mice

The ability of tranilast, a mast cell stabilizer and anti-transforming growth factor_β (TGF_β) to improve impaired hepatic functions in Schistosoma mansoni (S. mansoni)-infected mice, was investigated, providing the first evidence on the ability of tranilast to improve hepatic impairment due to schistosomal infection. Tranilast had significant beneficial effects against progression of hepatic fibrosis in S. mansoni-infected mice treated with praziquantel and those untreated. Different aspects of drug activity were investigated. Its effect on serum liver functions was evaluated by estimating: alanine aminotransferase, aspartate aminotransferase, total bilirubin, alkaline phosphatase and albumin. Its effect on the extent of liver fibrosis, through estimation of hepatic hydroxyproline and hepatic collagen content in liver hydrolysates, was also evaluated. Also, the expression of profibrogenic mediators, such as serum TGF_β1, was estimated. Finally, the effect on S. mansoni infection itself was studied, via histopathological examination of liver specimens stained with both hematoxylin–eosin and Masson’s trichome stains. Tranilast ameliorated the harmful effects of S. mansoni infection on the liver. Such action was manifested in its significant ability to improve impaired hepatic functions, reduce histopathological changes, lower hepatic collagen content and finally reduce serum TGF_β1 levels. The beneficial effect of tranilast may be in part due to its ability to reduce the production of profibrogenic mediators in the infected animals by improving the host immune response or by interfering with critical steps in the fibrogenic cascade.     

Effects of 3-aminobenzamide on poly(ADP-ribose)polymerase expression,apoptosis and cell cycle progression of HeLa cells after X-ray irradiation

The aim of this paper is to study the changes of apoptosis and cell cycle progression in HeLa cells after the poly (ADP-ribose) polymerase (PARP) was inhibited by its inhibitor 3-aminobenzamide (3-AB) and the mechanisms of PARP action on HeLa cells damaged by irradiation. Flow cytometry (FCM) was used to examine the PARP expression and the percentage of apoptotic cells and cell cycle progression. The percentage of HeLa cells with positive expression of PARP protein 2, 4, 8 and 12 h after administrated with 3-AB was significantly lower than that of the control (P < 0.01). The percentages of apoptotic cells in the 3-AB plus irradiation group at the time points of 2, 8, 12 and 24 h after 2 Gy irradiation were higher than that in the irradiation group (P < 0.01 or P < 0.05) and the percentage of G₂ cells decreased significantly (P < 0.01 or P < 0.05). It indicates that 3-AB can rapidly inhibit PARP expression of HeLa cells, promote cell apoptosis and block G₂ arrest induced by irradiation. __________ Translated from Journal of Jilin University (Medicine Edition), 2007, 33(3): 418–421 [译自: 吉林大学学报(医学版]     

High risk factors for pulmonary fungous infection in intensive care units of neurosurgery

By analyzing the high risk factors for pulmonary fungous infection in intensive care units of neurosurgery, the strategy of early diagnosis and treatment was explored. According to the domestic diagnostic standard on pulmonary fungous infection, clinical data on 58 patients with the infection in our department were analyzed. One hundred and seventeen strains of fungi were separated from the 58 cases. Candidiasis was the most frequent type, accounting for 92.3% of the cases. Conditions such as the severity of primary diseases, long-time coma, long-term use of broad-spectrum antibiotic, abuse of glucocorticoid, the open airway, and some invasive intubations, may be regarded as high risk factors for pulmonary fungous infection. Fluconazole showed good clinical effects on the treatment of fungous infection. To eliminate these high risk factors, early diagnosis and the use of prophylactic antifungal agents can help reduce the incidence of pulmonary fungous infection. Translated from Chinese Journal of Nosocomiology, 2006, 16(4): 385–388 [译自: 中华医院感染学杂志]     

The association of ABCB1 polymorphisms and elevated serum digitoxin concentrations in geriatric patients

Objective Digitoxin is a known substrate of the efflux pump P-glycoprotein (gene name: ABCB1). P-glycoprotein expression was shown to be modulated by single nucleotide polymorphisms in the ABCB1 gene, but it remains unclear whether these polymorphisms influence digitoxin blood levels. Our objective was to examine the association of ABCB1 C3435T genotype and elevated serum digitoxin concentrations (SDC) in a cohort of 77 geriatric patients consecutively admitted to a geriatric department over a 12-month period. Methods The impact of ABCB1 3435 CC, CT, and TT genotypes on SDC and SDC normalized for daily digitoxin dosage and body weight was assessed by multivariate regression analysis. Results Among participants, 18 (23%) had the CC, 36 (47%) the CT, and 23 (30%) the TT genotype. Adjusting for relevant covariates, no significant association of ABCB1 C3435T genotype and SDC or normalized SDC was detected. Mean SDC was 22.4 ng/ml (95% CI 18.9–25.9) for the TT, 21.8 ng/ml (95% CI 18.1–25.5) for the CT, and 25.7 ng/ml (95% CI 20.6–30.8) for the CC genotype. The means for normalized SDC were 5.2 kg·l⁻¹ (95% CI 4.3–6.1) for the TT, 6.1 kg·l⁻¹ (95% CI 4.7–7.5) for the CT, and 6.2 kg·l⁻¹ (95% CI 4.6–7.7) for the CC genotype. Conclusion In this sample of frail geriatric patients, the impact of ABCB1 C3435T genotype on serum digitoxin concentration was not of major relevance. Regular monitoring of digitoxin blood levels and surveillance of appropriate drug use remain the best ways to prevent digitoxin intoxications in the elderly.     

Measurement of bone alkaline phosphatase and relative study with osteosarcoma

The objective of this paper is to explore the value of bone alkaline phosphatase (BALP) for diagnosing osteosarcoma, evaluating the effect of the chemotherapy, judging the prognosis and supervising the relapse and metastasis. The immunoassay was used to check the BALP of the blood serum that was from 42 primary osteosarcoma patients. Alkaline phosphatase (ALP) in blood serum was checked with auto biochemistry equipment. The biopsy tissue and the lesion resected in operation were treated with pathology and histological response was counted. The patients were followed up from five months to 49 months with an average of 24.3 months. Eighteen cases relapsed and transferred, among which, 16 of them were dead, and others were survival to the end of the follow-up. BALP was more sensitive than ALP in diagnosing osteosarcoma (P = 0.015). Fifteen cases decreased to normal value in ALP after preoperative chemotherapy, and 34 cases decreased in BALP. Both ALP and BALP in all cases decreased to normal value in post-operative. There was significant difference in positive correlation between the decrease of BALP and the increase of histological response (P = 0.001, r = 0.642). In the follow-up, there was significant difference in BALP between the group of relapse and transfer and the group of free disease survival (P = 0.000). As a check marker in blood serum, BALP, reflecting the process of ossification, has a higher sensitivity than ALP. It has applied value in the diagnosis of osteosarcoma, reflection of the effect of chemotherapy and forecast the prognosis. Translated from Journal of Shandong University (Health Sciences), 2006, 44(6): 610–613 [译自: 山东大学学报 (医学版)]     

Involvement of p38 mitogen-activated protein kinase in the regulation of platelet-derived growth factor induced cell migration

The aim of this study was to investigate the role of p38 mitogen-activated protein kinase (MAPK) in cell migration induced by platelet-derived growth factor (PDGF). Western blot was performed to detect the phosphorylation of p38 in NIH3T3 cells treated with PDGF. A Transwell cell migration system was used to determine the effects of PDGF treatment on the migration of NIH3T3 cells and the influence of p38 deficiency on this process in a p38 gene knockout (p38^−/−) mouse embryonic fibroblast cell line. On the stimulation of PDGF, the migration of NIH3T3 cells was significantly increased (P < 0.001) compared to the control and p38 MAP kinase was simultaneously phosphorylated. Furthermore, the PDGF-induced cell migration was significantly blocked in p38 gene knockout (p38^−/−) mouse embryonic fibroblasts (MEFs) (P < 0.001) as compared with the wild type cells (p38^+/+). p38 MAPK plays an important role in the regulation of cell migration induced by PDGF. Translated from Medical Journal of Chinese People’s Liberation Army, 2007, 32(3): 205–207 [译自: 解放军医学杂志]