肺炎支原体液体培养方法的应用

目的对肺炎支原体（Mp）快速液体培养的方法进行分析,探讨其应用价值。方法采用Mp快速液体培养基对呼吸系统感染患者的咽拭子进行培养,根据颜色变化判断结果,分析真菌、肺炎克雷伯氏菌、大肠埃希菌、产气肠杆菌、铜绿假单胞菌、链球菌、葡萄球菌对结果的影响,然后用PCR荧光探针方法进行验证。结果真菌能在培养液中生长引起假阳性,其它细菌可被抑制,阳性培养液（无真菌）PCR荧光定量94％阳性,阴性培养液PCR荧光定量100％阴性。结论Mp快速液体培养的方法快速、简便,光学显微镜排除真菌后结果准确。     

肺炎支原体液体培养方法的应用

目的 对肺炎支原体(Mp)快速液体培养的方法 进行分析,探讨其应用价值.方法 采用Mp快速液体培养基对呼吸系统感染患者的咽拭子进行培养,根据颜色变化判断结果 ,分析真菌、肺炎克雷伯氏菌、大肠埃希菌、产气肠杆菌、铜绿假单胞菌、链球菌、葡萄球菌对结果 的影响,然后用PCR荧光探针方法 进行验证.结果 真菌能在培养液中生长引起假阳性,其它细菌可被抑制,阳性培养液(无真菌)PCR荧光定量94%阳性,阴性培养液PCR荧光定量100%阴性.结论 Mp快速液体培养的方法 快速、简便,光学显微镜排除真菌后结果 准确.     

快速液体培养法检测呼吸道标本中的肺炎支原体

目的 探讨快速液体培养法检测肺炎支原体(MP)的临床应用价值,分析其假阳性的情况及原因.方法 对93例呼吸道标本进行MP快速液体培养,培养液用荧光定量PCR(FQ-PCR)检测肺炎支原体核酸,同时测定血清中肺炎支原体抗体,进行统计学分析.结果 在93例检测标本中,肺炎支原体快速培养阳性16例,检出率是17.2%(16/93);FQ-PCR法阳性10例,检出率10.7%(10/93);血清学检测阳性22例,检出率23.6%(22/93).3种方法检测结果无显著差异.结论 快速液体培养法干扰因素多,影响其特异性;结果判断时结合显微镜检查,排除细菌或真菌等导致的假阳性,其特异性可达到与血清学及FQ-PCR法一致水平.     

快速培养检测肺炎支原体的临床价值

目的 了解快速培养基检测肺炎支原体性能及临床价值。方法 使用上海奥普生物医药公司产肺炎支原体液体培养基进行检测。结果 428份标本192例阳性,总检出率44.85％,其中男性检出率44.44％,女性检出率45.21％,经卡方检验P>0.05,男女无差异。检测结果91％于24 h内发出报告,最快6 h。结论 快速培养基检测肺炎支原体感染总检出率较高,结果选择性好,最快6 h,绝大多数24 h内检测出肺炎支原体,总的情况表明该培养基较传统培养基具有快速检测能力,为临床尽快诊断肺炎支原体感染,给患者及时有效地治疗有较高的价值。     

肺炎支原体快速液体培养的方法评价

目的对肺炎支原体(Mp)快速液体培养的方法进行分析评价,探讨其应用价值。方法采用Mp快速液体培养基对呼吸系统感染患者的咽拭子进行培养,根据颜色变化判断结果,然后用聚合酶链反应(PCR)荧光定量方法进行验证。结果阳性培养液PCR荧光定量100%阳性,阴性培养液PCR荧光定量96%阴性(其中4%PCR阳性的阴性培养液延长培养时间也出现了阳性结果)。结论Mp快速液体培养的方法结果准确、快速、简便,可作为一般实验室诊断Mp感染首选试验。     

肺炎支原体快速培养法在呼吸道疾病中的应用

目的探讨呼吸系统肺炎支原体感染的早期诊断。方法采用肺炎支原体快速液体培养法对320例临床拟诊为呼吸道感染患儿咽拭子及340例呼吸道感染的成人痰及咽拭子进行培养,并对320例呼吸道感染患儿作血清冷凝集试验及血清特异性抗体IgG检测。对检测结果和临床诊断进行分析。结果患儿肺炎支原体快速液体培养法阳性157例,阳性率49.1%;成人呼吸道感染咽拭子阳性112例,阳性率为32.9%,痰阳性28例,阳性率为8.2%;血清肺炎支原体IgG抗体阳性率为62.8%(201/320),冷凝集试验阳性率为35.6%(114/320)。结论肺炎支原体快速液体培养法快速、简便、敏感、特异,对肺炎支原体感染的早期诊断具有重要的参考价值,适合基层实验室开展应用。     

肺炎支原体液体培养假阳性原因分析及解决方法探讨

商品化的肺炎支原体液体培养基因其快速和经济的特点．在临床被广泛应用于初步筛检临床样本中有无肺炎支原体。但有文献报道此方法假阳性较高．我们在日常工作中也发现此方法假阳性的存在并曾做了报道．通过探索．发现在液体培养基中加入氟康唑可以大大减少液体培养法的假阳性．现报道如下。     

阴道分泌物支原体固体和液体培养基培养结果差异及原因分析

目的分析阴道分泌物支原体固体培养基和液体培养基培养结果差异及其原因,为临床医生科学解读阴道分泌物支原体通过不同方法培养后的结果提供试验依据。方法收集该院妇产科送检的354份阴道分泌物标本,同时做固体培养基和液体培养基培养,比较两种培养方法的阳性率,并将液体培养基培养阳性但固体培养基培养阴性的标本进一步做细菌和真菌鉴定,支原体液体培养基培养阴性但固体培养基培养阳性标本再进行液体培养。结果354份阴道分泌物标本中固体培养基培养阳性[解脲脲原体(Uu)或人型支原体(Mh)阳性]标本为112份,阳性率为31.64%。112份阳性标本中,单纯Uu阳性86份,阳性率为24.29%;单纯Mh阳性9份,阳性率为2.54%;Uu合并Mh阳性17份,阳性率为4.80%。液体培养基培养阳性率(40.96%)明显高于固体培养基培养阳性率(31.64%),差异有统计学意义(χ2=6.65,P<0.001)。结论支原体固体培养基培养阳性率明显低于液体培养基培养,通过观察固体培养基的菌落形态,可准确诊断Uu和Mh感染;分解尿素和精氨酸的细菌或真菌感染是造成支原体液体培养基假阳性的主要原因。     

肺炎支原体液体培养假阳性原因分析及解决方法探讨

商品化的肺炎支原体液体培养基因其快速和经济的特点,在临床被广泛应用于初步筛检临床样本中有无肺炎支原体.但有文献报道此方法假阳性较高 [1-4],我们在日常工作中也发现此方法假阳性的存在并曾做了报道 [5].通过探索,发现在液体培养基中加入氟康唑可以大大减少液体培养法的假阳性,现报道如下.     

不同培养法检测泌尿生殖道分泌物支原体结果分析及评价

目的:对液体培养法与固体培养法对泌尿生殖道分泌物支原体培养结果的可信性进行分析,为临床实际应用提供指导意见.方法:应用液体培养基加药物敏感试验法和固体培养法培养泌尿生殖道分泌物中支原体,分析2种方法的优缺点.结果:2种培养基对解脲支原体和人型支原体的检测结果均为阳性26例,阴性63例,具有高度的一致性(μ=9 091,P＜0.01).但也存在不一致的情况,表现为液体培养对人型支原体检测存在假阴性结果(7例).结论:液体培养法具有简单、快速、经济等优点,但在人型支原体鉴定上有不足之处,要加强与固体培养方法的配合应用.     

Evaluation of the BDProbeTec ET system as screening tool in the direct detection of mycobacterium tuberculosis complex in respiratory specimens

We evaluated the BDProbeTec ET System (Becton Dickinson) for the routine detection of Mycobacterium tuberculosis complex (MTC) in respiratory specimens and pleural fluids, comparing with microscopy (Ziehl Neelsen stain, ZN) and culture in liquid (BACTEC MGIT 960, MGIT) and solid (L?wenstein Jensen, LJ) media. Five hundred and two specimens, collected from 266 patients, of which 257 with suspected tuberculosis and 9 receiving anti-tuberculosis treatment, were investigated. Thirty-nine specimens were positive by any method, including false positives. Mycobacteria were isolated from 33 specimens (32 Mycobacterium tuberculosis and 1 Mycobacterium chelonae). Thirty-six specimens were BDProbeTec ET positive, 33 specimens were MGIT positive, 27 were LJ positive and 22 were ZN positive. With BDProbeTec ET, 2 specimens were false negative (culture positive), and 2 specimens from non-treated patients were false positive (culture negative). The overall sensitivity, specificity, and positive and negative predictive values for BDProbeTec ET compared to culture were 93.7, 98.7, 83.3, and 99.5%, respectively, while with smear-positive and smear-negative specimens the sensitivities were 100% and 81.5% respectively. In five treated patients the disappearance of MTC could be monitored using BDProbeTec ET in parallel with culture. The overall inhibition rate was 0.2%. BDProbeTec ET can be very useful for rapid detection of MTC, especially in smear-negative respiratory specimens.     

A comparison of Binax NOW to viral culture and direct fluorescent assay testing for respiratory syncytial virus

The Binax NOW immunochromatographic assay for respiratory syncytial virus was prospectively compared with direct fluorescent assay and viral culture at Primary Children's Medical Center, Salt Lake City, Utah during February 2003. Three hundred ten patient specimens were collected for testing, of which 102 specimens were positive for respiratory syncytial virus by the reference tests, direct immunofluorescence assay (DFA), and culture or molecular analysis. DFA analysis identified an additional 40 patient specimens positive for other respiratory viruses. Compared to the reference tests, the sensitivity, specificity, and positive and negative predictive values of the rapid immunochromatographic assay for detection of respiratory syncytial virus were 89.2%, 100.0%, 100.0%, and 94.9%, respectively. This rapid assay format proved to be cost-effective and simple to use in comparison to DFA and viral culture. Negative rapid test results should still be confirmed with a secondary test.     

分离培养和血清学试验诊断肺炎支原体早期感染的应用及评价

目的应用并评价肺炎支原体（MP）快速培养法、被动凝集法检测总抗体和酶联免疫吸附试验（ELISA）检测免疫球蛋白M（IgM）抗体在MP感染早期诊断中的价值。方法应用MP快速培养法对该院2009年10月至2010年4月260例急性下呼吸道感染住院患者的咽拭子标本进行培养,同时用被动凝集法检测患者血清中MP的总抗体和ELISA检测MP的IgM抗体。结果260例急性下呼吸道感染患者咽拭子MP快速签定培养法培养阳性58例。阳性率22。3％。被动凝集法检测总抗体阳性83例．阳性率31．9％。ELISA检测IgM抗体阳性67例,阳性率25．8％。结论MPIgM抗体检测试剂因其方便,快捷,可作为优先检测试验。     

细菌快速检测试纸片法与传统方法培养结果的比较

目的比较细菌快速检测试纸片法与传统的方法在细菌检测结果上的差异。方法用试纸片法对物体表面污染菌总数和大肠杆菌进行检测,同时与传统方法作平行比较。结果检测细菌总数,用常规方法培养48 h后,细菌生长正常;而纸片法培养48 h,营养琼脂平板上无菌生长,继续培养至72 h才出现菌落。大肠菌群检测中,传统9管发酵法培养24h即有菌生长,国产大肠菌群快速检测试纸法培养36 h和日本产试纸片培养48 h才出现肉眼可见菌落。结论细菌快速检测试纸片虽能简化操作,但存在技术缺陷,且成本高。     

Evaluation of the BDProbeTec ET DTB assay(1) for direct detection of Mycobacterium tuberculosis complex from clinical samples

The purpose of this study was to evaluate the ability of BDProbeTec ET DTB system to detect Mycobacterium tuberculosis complex directly from clinical specimens. A total of 628 specimens (553 respiratory and 75 non respiratory specimens) were collected from 478 patients. These samples were tested with the BDProbeTec ET DTB assay and results were compared with acid fast microscopy and culture. Sixty eight out of 77 culture positive M. tuberculosis complex samples were detected with overall sensitivity and specificity of 89.5% and 98.2% respectively. Overall sensitivity was 100% in smear positive samples and 79% in smear negative samples. After resolution of discrepant results, sensitivity and specificity for respiratory samples were 91.6% and 98.7% respectively. BDProbeTec ET DTB assay demonstrated to be a rapid, sensitive and specific method for detection of M. tuberculosis complex.     

门诊病人解脲支原体培养以及药敏状况分析

目的探讨门诊病人非淋病性尿道炎解脲支原体培养方法以及药敏状况,指导临床用药。方法收集2009年性病门诊就诊患者的泌尿生殖道标本共250例,分别进行固体培养基和液体培养基的培养,并对药敏状况进行分析。结果液体培养基法检测出阳性85例,固体培养基法检测出阳性70例,液体培养基阳性而固体培养基阴性者23例,固体培养基阳性而液体培养基阴性者8例。进行一致性检验,两组方法有较强的吻合度(Kappa=0.758),有统计学意义(P<0.01)。解脲支原体对四环素类以及大环内酯类药品耐药率较高。结论解脲支原体的检测以液体培养基进行筛选,以固体培养基进行确诊,可大幅度提高检测结果的精确性。解脲原体对司帕沙星、克拉霉素、交沙霉素、美满霉素等药物敏感。     

A comparison of direct immunofluorescence, shell vial culture, and conventional cell culture for the rapid detection of influenza A and B

Direct immunofluorescence (FA) and shell vial contrifugation cultures (SVCs) were compared with conventional tube cultures for the rapid detection of influenza A and B by using a commercial antibody. Of the 439 specimens tested, 82 were positive by conventional culture (CC). The direct smear prepared from pelleted cells or direct swab material exhibited positive fluorescence in only seven (8.5%) of these cases, whereas the SVC was positive in 30 (37%). The SVC method detected 12 additional positive isolates that were not recovered in CC. The mean time to isolation in CC was 3.6 days for influenza A and 4.3 days for influenza B. The use of SVC provided more rapid results (36-48 hr). The FA method, although more rapid, may be of limited sensitivity and difficult to interpret depending on the quality of the specimen. The results indicate that SVC complements conventional culture in the rapid detection of influenza and can detect infections that may be missed in conventional tubes, but should not be used to the exclusion of conventional culture.