乌贼墨对高脂血症大鼠血脂代谢和抗氧化能力的影响

目的 探讨乌贼墨对实验性高脂血症大鼠血脂代谢和抗氧化能力的影响。方法 饲喂高脂饲料建立高脂血症大鼠模型，同时灌胃乌贼墨，剂量分别为50和200mg·kg^-1，连续30d。分别测定大鼠血清总胆固醇（TC），甘油三酯（TG），低密度脂蛋白胆固醇（LDL—c），高密度脂蛋白胆固醇（HDL-c）和一氧化氮（NO）含量，以及大鼠血清和肝脏丙二醛（MDA）含量，超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GSH—Px）活性。结果乌贼墨能显著性降低模型大鼠血清中的TC，TG和LDL-c含量，升高HDL-C，降低动脉粥样硬化指数（LDL-c／HDL-c）；显著提高高脂血症大鼠血清中NO含量；显著降低血清及肝组织中MDA含量，提高SOD和GSH—Px活性。结论乌贼墨可通过调节机体血脂代谢、提高机体内源性抗氧化酶活性，降低机体的过氧化水平，有效预防高脂血症的形成。     

北极刺参和日本刺参对大鼠血脂水平调节和血管内皮保护作用的研究

目的比较研究北极刺参和日本刺参对实验性高脂血症大鼠血脂水平调节和血管内皮细胞的保护作用。方法采用高脂饲料饲喂法建立高脂血症大鼠模型，同时分别灌胃不同剂量（50、100和200mg／kg）的北极刺参与日本刺参匀浆，30d后测定大鼠血清TC，TG，LDL—C．HDL-C和NO含量，以及血清和肝脏MDA含量、SOD和GSH—Px活性。结果北极刺参和日本刺参均能明显降低模型大鼠血清TC，LDL—C含量和动脉粥样硬化指数（P〈0．01），提高HDL—C和NO（P〈0．01）含量；显著降低血清和肝脏中MDA含量（P〈0．05，P〈0．01），提高血清SOD和GSH—Px（P〈0．01）活性。其中．日本刺参降低大鼠LDL-C水平和动脉粥样硬化指数的作用显著优于北极刺参（P〈0．05）。结论2种刺参均能显著调节机体的血脂水平，提高抗氧化能力和保护血管内皮细胞功能，有效预防高脂血症和动脉粥样硬化的形成。     

银杏内酯B对实验性动脉粥样硬化的抗氧化作用研究

目的：探讨银杏内酯B对动脉粥样硬化（AS）大鼠的抗氧化作用.方法：采用高脂饲料喂养和维生素D3腹腔注射的方法建立大鼠AS模型,分为正常组、模型组、阳性组（辛伐他汀5mg· kg-1）、银杏内酯B三个剂量组（8,4,2 mg·kg-1）,每组10只.灌胃给药4周后,各组取主动脉,光镜下观察病理改变,进行病变分级评分;取血清,测定血脂水平、超氧化物歧化酶（SOD）活力和丙二醛（MDA）含量.结果：银杏内酯B能改善AS大鼠的主动脉病变,高剂量组与模型组比较有统计学意义（P＜0.05）.银杏内酯B高、中、低剂量可分别降低AS大鼠血清三酰甘油（TG）、总胆固醇（TC）、低密度脂蛋白胆固醇（LDL-C）水平,银杏内酯B高、中剂量可显著升高AS大鼠血清高密度酯蛋白胆固醇（HDL-C）水平,与模型组比较,差异均有统计学意义（P＜0.05或0.01）.银杏内酯B各剂量能显著升高SOD活性,降低AS大鼠血清MDA含量,与模型组相比,差异有统计学意义（P＜0.05或0.01）.结论：银杏内酯B的抗AS作用可能与增强SOD活性、减少MDA含量、减轻氧自由基的损伤有关.     

加味二至胶囊对大鼠复合型脂肪肝的调脂保肝作用

目的：研究加味二至胶囊对大鼠复合型脂肪肝的调脂保肝作用。方法：采用高脂饲料、酒精及四氯化碳复制大鼠复合型脂肪肝模型,实验分为空白对照组、模型组、加味二至胶囊高剂量组（MEP1）、加味二至胶囊中剂量组（MEP2）、加味二至胶囊低剂量组（MEP3）及东宝肝泰组（DB）,然后测定各组肝脏指数,血清脂质TC、TG、HDL及LDL含量,测定血清ALT及AST含量,肝匀浆中游离脂肪酸、SOD及MDA含量。结果：模型组大鼠肝脏指数,血清脂质TC、TG、LDL,血清转氨酶ALT、AST含量较空白对照组明显升高,HDL较空白对照组显著降低;与空白对照组相比模型组大鼠SOD活性显著降低,MDA含量显著升高。与模型组相比各治疗组肝脏指数,血清脂质TC、TG、LDL,血清转氨酶ALT、AST含量均显著降低,HDL含量显著升高。与模型组相比,各治疗组SOD活性显著提高,MDA含量明显降低。结论：加味二至胶囊具有较好的降脂保肝作用,其作用机制可能与提高抗氧化能力有关。     

两种微藻油对高脂小鼠脂类代谢及脂质过氧化作用的影响

目的探讨两种海洋微藻油(小球藻油和等边金藻油)对饲喂高脂饲料小鼠血脂水平及脂质过氧化的影响。方法 100只小鼠分为5组,分别为对照组,高脂模型组,小球藻油组,等鞭金藻油组及鱼油组。饲喂高脂饲料及相应药物6W后,测血脂水平及肝脏和血清的丙二醛(MDA)水平及过氧化氢酶(CAT)、超氧化物歧化酶(SOD)活性。结果两种微藻油可使高脂小鼠血清甘油三酯(TG)、总胆固醇(TC)、低密度脂蛋白胆固醇(LDL-C)含量降低,而高密度脂蛋白胆固醇(HDL-C)升高。与模型组相比,两种微藻油处理组的血清和肝组织的SOD、CAT活性明显增强,脂质过氧化产物MDA含量下降。而两种微藻之间,以小球藻油具有较高的SOD和CAT活性。结论两种微藻油具有显著调血脂作用,并可增强体内抗氧化酶活性,降低因高脂饲料引起的小鼠脂质过氧化。     

金正胶囊对大鼠血脂水平的影响

目的：观察金正胶囊对大鼠血脂水平的影响。方法根据样品的人体推荐剂量,设126．5、253．0、506．0mg? kg －13个剂量组,再设一个基础饲料对照组（空白对照）和一个高血脂模型对照组（高脂对照）,每组12只动物,对比各组大鼠血清总胆固醇（TC）、甘油三酯（TG）、高密度脂蛋白胆固醇（HDL－C）含量变。结果126．5、253．0、506．0mg?kg－13个剂量组大鼠的血清TC、TG含量均低于高脂对照组,其差异具有统计学意义（P＜0．01,P＜0．05）。结论金正胶囊对高脂血症大鼠血脂有明显降低作用。     

茵陈蒿汤对慢性酒精性脂肪肝的疗效及机制研究

目的 探讨茵陈蒿汤对模型大鼠酒精性脂肪肝的干预作用.方法 60只SD大鼠随机分成正常对照组、模型对照和茵陈蒿汤组,模型对照和茵陈蒿汤组大鼠饮用10%乙醇、加高脂高热量饲料喂养6周造成大鼠酒精性脂肪肝模型 茵陈蒿汤组大鼠灌胃给药茵陈蒿汤0.1g/kg,继续酒精和高脂高热量饲料处理4周后处死.测定血清丙氨酸氨基转移酶（ALT）、天冬氨酸氨基转移酶（AST）、碱性磷酸酶（ALP）和甘油三酯（TG）、胆固醇（TC）、高密度脂蛋白胆固醇（HDL-C）、低密度脂蛋白胆固醇（LDL-C）以及大鼠肿瘤坏死因子-α（TNF-α）、转化生长因子-β1（TGF-β1）含量 测定肝匀浆TG、丙二醛（MDA）、超氧化歧化酶（SOD）、还原性谷胱甘肽（GSH）水平,并进行肝组织病理学检查.结果 茵陈蒿汤能抑制病鼠血清ALT、AST、ALP活性和降低TG、LDL-C、TNF-α、TGF-β1水平,提高HDL-C含量 降低肝匀浆TG和MDA含量,增强SOD活性以及改善肝细胞空泡变性和脂肪变性.结论 茵陈蒿汤阻止大鼠酒精性脂肪肝形成,其作用机制包括：调血脂、减少脂肪在肝脏的沉积 抗氧化、清除自由基代谢产物,抑制脂质过氧化反应 抗免疫性炎症和减少细胞外基质沉积.     

番茄红素对大鼠非酒精性脂肪肝的作用研究

目的观察番茄红素对非酒精性脂肪肝的影响并探讨其作用机制。方法用高脂饲料诱导建立大鼠脂肪肝模型,再给予番茄红素治疗,观察各组大鼠血清或组织中天门冬酸氨基转移酶（AST）、谷氨酸氨基转移酶（ALT）、超氧化物歧化酶（SOD）、丙二醛（MDA）、甘油三酯（TG）、胆固醇（CHO）、高密度脂蛋白胆固醇（HDL—C）、低密度脂蛋白胆固醇（LDL—C）、游离脂肪酸（FFA）、胆碱酯酶（CHE）的活性或含量。并观察肝组织病理学改变情况。结果与自然恢复组比较,番茄红素给药组血清AST及ALT的活性显著降低;肝组织中SOD活性升高、MDA含量降低;血清中TG,CHO,LDL—C及FFA水平降低,HDL—C水平升高;血清及肝组织中的CHE活性降低;肝脏脂肪沉积减少,肝组织的病理改变显著。结论番茄红素能够减轻非酒精性脂肪肝的病变程度,其机制可能与其所具有的调血脂及抗氧化等作用有关。     

松针原花青素对高脂大鼠血清中SOD活性、MDA含量的影响

目的探讨松针原花青素对Wistar高脂大鼠血清中超氧化物歧化酶（SOD）、丙二醛（MDA）的影响。方法将40只约40天龄雄性Wistar大鼠随机分为普通饮食组（A）、高脂对照组（B）、高脂＋松针原花青素低（C）、高剂量组（D）,低剂量组100mg／（kg·d）,高剂量组300rag／（kg·d）,每组10只。分别于0、4、8周眦静脉取血测定血清中SOD、MDA的含量。结果8周末时,D组大鼠血清中sOD含量较B组明显升高（P〈0．05）,MDA水平较B组明显降低（P％0．05）;而C组大鼠血清中SOD含量较B组有所升高,差异无统计学意义（P〉0．05）,MDA含量较B组有所降低,差异无统计学意义（P〉0．05）。结论松针提取物具有减缓Wistar大鼠动脉粥样硬化形成的作用,这可能与其提高大鼠血清sOD活性、阻抑MDA升高、减低对血管内皮细胞损伤及保护血管内皮功能等作用有关。较高剂量的松针原花青素能有效改善大鼠的抗氧化能力,原花青素提取物具有降低血液MDA含量、提高血清SOD活力的作用,对其作用机制的进一步研究,可为动脉粥样硬化的治疗提供新的思路和依据。     

南极磷虾油对高脂血症大鼠血脂和抗氧化力的影响

目的探讨南极磷虾油对实验性高脂血症大鼠血脂和抗氧化力的影响。方法高脂饲料建立高脂血症大鼠模型,分别灌胃50,100和500 mg.kg-1南极磷虾油,连续30 d,测定大鼠血清总胆固醇(TC),甘油三酯(TG),低密度脂蛋白胆固醇(LDL-C),高密度脂蛋白胆固醇(HDL-C)、血清一氧化氮(NO)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-PX)以及丙二醛(MDA)水平。结果南极磷虾油能显著降低高脂血症大鼠血清中的TC、TG和LDL-C含量,降低动脉粥样硬化指数(AI)。提高大鼠血清中NO含量,提高SOD和GSH-PX活性,降低MDA含量。结论南极磷虾油对高脂血症大鼠具有调血脂的作用和抗氧化作用,其抵抗动脉粥状硬化方面的作用优于深海鱼油。     

Effects of conjugated linoleic acid supplementation on blood lipids and adiposity of rats fed diets rich in saturated versus unsaturated fat

Conjugated linoleic acid (CLA) may decrease adiposity and improve blood lipid profiles under some conditions. The goal of this study was to determine the effects of CLA supplementation on blood lipid profiles and adiposity of rats fed a diet containing a primarily saturated fat versus a diet containing a primarily unsaturated fat. Twenty-eight male Sprague-Dawley rats were randomly assigned to one of four diets containing coconut oil, coconut oil with CLA, corn oil or corn oil with CLA. After 28 days, blood was collected and serum concentrations of total cholesterol (TC), HDL-cholesterol (HDL-C), and triacylglycerols (TG) were assessed. Food intake, body weights, and epididymal fat pads were measured. No significant differences (p>0.05) were noted among groups for amount of food consumed, weight gained, food efficiency ratio or serum TG concentrations. TC concentrations were lower (p<0.05) in the CLA-supplemented rats that were fed coconut oil but not those consuming corn oil. Serum HDL-C was lower (p<0.05) in rats consuming corn oil but was not significantly different (p>0.05) for CLA supplemented groups. Epididymal fat pads weighed significantly more (p<0.05) in the coconut oil fed group compared to the corn oil fed group, but there was no significant difference (p>0.05) between the corn oil and coconut oil + CLA group. Overall, this study suggests that CLA is more beneficial for control of blood lipids and adiposity when supplemented to a diet rich in saturated versus unsaturated fat.     

Protective Effect of Gymnema sylvestre Ethanol Extract on High Fat Diet-induced Obese Diabetic Wistar Rats

Obesity is associated with numerous co-morbidities such as cardiovascular diseases, type 2 diabetes, hypertension and others. Therefore, the present study was planned to investigate the effect of water- soluble fraction of Gymnema sylvestre ethanol extract on biochemical and molecular alterations in obese diabetic rats. Diabetes was induced by single i.v. injection of streptozotocin (45 mg/kg) via tail vein. Obesity was induced by oral feeding of high fat diet for a period of 28 days in diabetic rats. Body weight gain, food intake, water intake, hemodynamic parameters (systolic, diastolic, mean arterial blood pressures and heart rate), serum biochemical parameters (leptin, insulin, lipid levels, apolipoprotein B and glucose), cardiomyocyte apoptosis (cardiac caspase-3, Na⁺/K⁺ ATPase activity and DNA fragmentation) organs and visceral fat pad weight and oxidative stress parameters were measured. Oral treatment with water soluble fraction of Gymnema sylvestre ethanol extracts (120 mg/kg/p.o.) for a period of 21 days, resulted in significant reduction in heart rate, mean arterial pressure, serum leptin, insulin, apolipoprotein B, lipids, glucose, cardiac caspase-3 levels, Na⁺/K⁺ ATPase activity and DNA laddering, visceral fat pad and organ''s weight and improved the antioxidant enzymes levels in the high fat diet induced obesity in diabetic rats. The results of present study reveal that water soluble fraction of Gymnema sylvestre ethanol extract could be useful intervention in the treatment of obesity and type-2 diabetes mellitus.     

Hypolipidemic and antioxidant effects of mulberry (Morus alba L.) fruit in hyperlipidaemia rats

The phytochemical constituents of a freeze-dried powder of mulberry (Morus alba L.) fruit (MFP) were determined. The hypolipidemic and antioxidant effects of the MFP as a dietary supplement were evaluated in rats who were fed 4 weeks of either a high-fat or a normal diet supplemented with 5% or 10% MFP. Administration of MFP to rats on a high-fat diet resulted in a significant decline in levels of serum and liver triglyceride, total cholesterol, serum low-density lipoprotein cholesterol, and a decrease in the atherogenic index, while the serum high-density lipoprotein cholesterol was significantly increased. In addition, the serum and liver content of thiobarbituric acid related substances, a lipid peroxidation product, significantly decreased, while the superoxide dismutase (SOD) of red blood cell and liver, as well blood glutathione peroxidase (GSH-Px) activities significantly increased. No significant changes in lipid profile in the serum and liver were observed in rats on a normal diet supplemented with MFP, but blood and liver antioxidant status improved, as measured by SOD and GSH-Px activity, and lipid peroxidation was reduced. These beneficial effects of MFP on hyperlipidaemia rats might be attributed to its dietary fiber, fatty acids, phenolics, flavonoids, anthocyanins, vitamins and trace elements content.     

Morin alleviates fructose-induced metabolic syndrome in rats via ameliorating oxidative stress,inflammatory and fibrotic markers

Metabolic syndrome (MBS) is a widespread disease that has strongly related to unhealthy diet and low physical activity, which initiate more serious conditions such as obesity, cardiovascular diseases and type 2 diabetes mellitus. This study aimed to examine the therapeutic effects of morin, as one of the flavonoids constituents, which widely exists in many herbs and fruits, against some metabolic and hepatic manifestations observed in MBS rats and the feasible related mechanisms. MBS was induced in rats by high fructose diet feeding for 12 weeks. Morin (30 mg/kg) was administered orally to both normal and MBS rats for 4 weeks. Liver tissues were used for determination of liver index, hepatic expression of glucose transporter 2 (GLUT2) as well as both inflammatory and fibrotic markers. The fat/muscle ratio, metabolic parameters, systolic blood pressure, and oxidative stress markers were also determined. Our data confirmed that the administration of morin in fructose diet rats significantly reduced the elevated systolic blood pressure. The altered levels of metabolic parameters such as blood glucose, serum insulin, serum lipid profile, and oxidative stress markers were also reversed approximately to the normal values. In addition, morin treatment decreased liver index, serum liver enzyme activities, and fat/muscle ratio. Furthermore, morin relatively up-regulated GLUT2 expression, however, down-regulated NF-κB, TNF-α, and TGF-β expressions in the hepatic tissues. Here, we revealed that morin has an exquisite effect against metabolic disorders in the experimental model through, at least in part, antioxidant, anti-inflammatory, and anti-fibrotic mechanisms.     

Safety assessment of DHA-rich microalgae from Schizochytrium sp

The purpose of this study was to determine the potential toxicity of docosahexaenoic acid-rich microalgae from Schizochytrium sp. (DRM), administered in the diet to rats for at least 13 weeks. DRM was administered in the diet to groups of 20 male and 20 female Sprague-Dawley derived rats (Crl:CD(SD)BR) to provide dosages of 0, 400, 1500, and 4000 mg/kg/day for at least 13 weeks. DRM contained high levels of fat (approximately 41% w/w) of which long-chain highly unsaturated fatty acids (PUFAs) were a major component. Vitamin E acetate was added to DRM at manufacture to provide supplementary dietary antioxidant given the highly unsaturated fat content of DRM. Untreated controls received the basal diet only. An additional group of 20 males and 20 females received basal diet mixed with fish oil (Arista) to provide a target dosage of 1628 mg/kg/day, an amount of fat comparable to that received by rats administered the highest dose of DRM. Vitamin E acetate was also added to the fish oil to provide a comparable level of dietary antioxidant provided to high-dose DRM rats. There were no treatment-related effects in clinical observations, body weights or weight gains, food consumption, hematologic or urinalysis values, gross necropsy findings, or organ weights and there were no deaths. The only treatment-related changes in clinical chemistry parameters were decreases in high-density lipoproteins and cholesterol in the DRM and fish oil groups when compared to the untreated controls. These changes were expected based on the high PUFA content of DRM and fish oil. There were no microscopic findings suggestive of toxicity. Periportal hepatocellular fat vacuolation (accumulation of fat) was observed only in the livers of female rats in both the DRM (all dosages) and fish oil groups. This finding was expected given the higher fat content of both the DRM and the fish oil diets compared to the basal diet fed to the untreated controls. A slight increase in the incidence, but not severity, of cardiomyopathy was observed only in the 4000 mg/kg/day DRM males. This finding was not considered adverse because cardiomyopathy occurs spontaneously in rats and especially male rats of the Sprague-Dawley strain when fed high levels of fat. Since cardiomyopathy does not develop in other species including primates fed high-fat diets, its occurrence in rats is considered to have little relevance to human health. This study demonstrates that administration of DRM did not produce any treatment-related adverse effects in Sprague-Dawley rats of relevance to humans at dosages up to 4000 mg/kg/day for 13 weeks.     

何首乌二苯乙烯苷类提取物对高脂血症大鼠血脂代谢的影响及其抗氧化作用

目的研究何首乌二苯乙烯苷类提取物对实验性高脂血症大鼠血脂代谢的影响及抗氧化作用。方法以喂饲法建立高脂血症大鼠模型。分别灌胃给予何首乌二笨乙烯苷类提取物（12,24mg／kg）,给药4周后,测定血清血脂、载脂蛋白含量及抗氧化作用指标。结果何首乌二苯乙烯苷类提取物能明显降低血清总胆固醇（TC）、甘油三酯（TG）、低密度脂蛋白胆固醇（LDL—c）、载脂蛋白（apoB）、脂质过氧化物（Mr）a）含量,使动脉粥样硬化指数（AI）降低,使高密度脂蛋白胆固醇（HDL—c）／TC及apoAI／apoB比值升高,并能提高高脂血症大鼠血清超过氧化物歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GSH—PX）活性及总抗氧化能力（T—AOC）。结论何首乌二笨乙烯苷类提取物能调节高脂血症大鼠血脂代谢及纠正自由基代谢紊乱,发挥抗动脉粥样硬化作用。     

An investigation of the effects of methylmercury in rats fed different dietary fats and proteins: testicular steroidogenic enzymes and serum testosterone levels.

Methylmercury (MeHg) is a testicular toxicant causing reduced steroidogenic enzyme activity, reduced serum testosterone (T) and abnormal spermatogenesis in mammals and fowl. It is also known that certain diets can alter androgen metabolism in rats. Previously we have shown that diets used in the current study impact circulating androgen levels and testicular steroidogenic enzyme activities in Sprague Dawley rats in the absence of MeHg. In the present study, we have investigated the impact of imposing an environmental contaminant (MeHg) commonly found in marine mammals and fish onto the rats' dietary intake of different proteins and lipids in order to determine if the different diets could modify MeHg toxicity in rats. Therefore, we examined the effects of MeHg on testicular steroidogenic enzymes and serum testosterone in rats fed diets containing either different protein sources (casein, fishmeal, whey) or different lipid sources (soybean oil, docosahexaenoic acid (DHA), seal oil, fish oil, lard). Male rats 42-45 days of age (18 per group) were assigned to different experimental diets for 28 days after which 6 rats in each group were gavaged daily with 0, 1 or 3 mg/kg body weight (BW)/day MeHg chloride in 5 mM Na(2)CO(3) solution for 14 days while being maintained on their diets. On the 43rd day of dosing, rats were sacrificed and blood plasma and testes frozen (-80 degrees C) until analysis. Microsomal steroidogenic enzyme activities (3beta-HSD, 17-OHase, C-17, 20-lyase, 17beta-HSD) were measured radiometrically. Serum testosterone was determined using ELISA kits. Testis weights were not affected by MeHg. MeHg at 3 mg/kg BW/day caused a reduction (>50%) in the activity of C-17, 20-lyase in all three protein diets and similar reductions in 17-OHase activity were seen in the casein and whey protein fed rats. At 3 mg/kg BW/day, MeHg reduced 17-OHase activity in the DHA diet but had no effect on 3beta-HSD activity and no inhibitory effects on 17beta-HSD activity. MeHg (3 mg/kg BW/day) caused significant reductions in serum T in the whey, soybean oil and fish oil groups. Interestingly, fishmeal protein but not fish oil offered some protection with respect to maintaining steroidogenic enzyme activities and serum T levels in rats dosed with MeHg. In conclusion, these studies show that different lipid diets can alter the toxic effects of MeHg on male rat steroidogenesis in terms of serum testosterone and steroidogenic enzyme activities.     

大黄酸固体分散体的制备及对大鼠血脂的影响

目的以聚乙烯吡咯烷酮为载体制备大黄酸固体分散体,考察其对大鼠血脂及抗氧化能力的影响。方法差示扫描量热法和红外光谱法对固体分散体进行分析。利用高脂饲料及脂肪乳剂建立SD大鼠高脂血症动物模型,大鼠分别灌胃给予大黄酸及大黄酸固体分散体,连续给药8周后测定总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)和丙二醛(MDA)含量,测定超氧化物岐化酶(SOD)和谷胱甘肽过氧化物酶(GSH-Px)的活力。结果大黄酸固体分散体能显著降低高脂血症大鼠血清中TC、TG、LDL-C含量(P<0.05);升高血清中HDL-C含量(P<0.05);提高SOD和GSH-Px活力;降低MDA含量(P<0.05)。结论以聚乙烯吡咯烷酮为载体制备的大黄酸固体分散体对高脂血症大鼠有降血脂及抗氧化作用。     

Evaluation of hypolipidemic activity of leaf juice of Catharanthus roseus (Linn.) G. Donn. in guinea pigs

Our aim of the study was to evaluate the hypolipidemic activity of leaf juice of Catharanthus roseus (Linn.) G. Donn. in guinea pigs. Adult guinea pigs of either sex were divided into seven groups: group 1 - normal diet; group 2 - high fat diet; group 3 and 4 - normal diet plus leaf juice of Catharanthus roseus (Linn.) G. Donn. in the dose of 0.5 and 1 mL/kg, respectively; group 5 and 6- high fat diet with leaf juice of Catharanthus roseus (Linn.) G. Donn. in the dose of 0.5 and 1 mL/kg, respectively; group 7 - high fat diet plus atorvastatin (3 mg/kg). Above diet treatment was given for six weeks and drug was given during last three weeks. Serum lipid profile (total cholesterol, triglycerides, LDL-c, VLDL-c, HDL-c) was performed in each group of animals before and at the end of six weeks. Histological study of aorta, liver and kidney was done in group 1, 2, 6 and 7 and blood cell count was done in animals that were treated juice of C. roseus (Linn.) G. Donn. before and after juice administration. Simultaneous administration of leaf juice of C. roseus (Linn.) G. Donn. in the dose of 0.5 mL/kg prevents the rise of serum lipid parameters and decreases the fatty changes in the tissue induced by high fat diet, whereas in the dose of 1 mL/kg not only counteracts the elevation, but also significantly (p < 0.05) reduces the serum level LDL-c and the ratio of total cholesterol and HDL-c. Leaf juice of C. roseus (Linn.) G. Donn. possesses significant lipid lowering and anti atherosclerotic activity.     

High dose of Garcinia cambogia is effective in suppressing fat accumulation in developing male Zucker obese rats, but highly toxic to the testis.

We investigated the ability of Garcinia cambogia extract containing (-)-hydroxycitric acid (HCA) to suppress body fat accumulation in developing male Zucker obese (fa/fa) rats. We also examined histopathologically the safety of its high doses. Diets containing different levels of HCA (0, 10, 51, 102 and 154 mmol/kg diet) were fed to 6-week-old rats for 92 or 93 days. Each diet group was pair-fed to the 154 mmol HCA/kg diet group. Epididymal fat accumulation and histopathological changes in tissues were observed. The highest dose of HCA-containing Garcinia cambogia (154 mmol HCA/kg diet) showed significant suppression of epididymal fat accumulation in developing male Zucker obese rats, compared with the other groups. However, the diets containing 102 mmol HCA/kg diet and higher (778 and 1244 mg HCA/kg BW/d, respectively) caused potent testicular atrophy and toxicity, whereas diets containing 51 mmol HCA/kg diet (389 mg HCA/kg BW/d) or less did not. Accordingly, 51 mmol HCA/kg diet (389 mg HCA/kg BW/d) was deemed to be the no observed adverse effect level (NOAEL).