Expressions of Beta-Catenin,SUFU and VEGFR-2 Proteins in Medulloblastoma

Objective:to investigate the expressions of beta-catenin,SUFU and VEGFR-2 proteins in medulloblastoma.Methods:Immunohistochemical staining with SP method was conducted to determine the expressions of beta-catenin,SUFU and VEGFR-2 in 33 cases of medulloblastoma and 10 normal cerebellar tissues.Results:the abnormal expression rates of beta-catenin and VEGFR-2 in medulloblastoma were significantly higher than that in normal tissue.While the positive expression of SUFU gene in medulloblastoma was significantly lower than that in 10 normal cerebellar tissues.A significant negative correlation was found between beta-catenin and SUFU proteins and a positive correlation between beta-catenin and VEGFR-2 was found in medulloblastoma.Conclusion:Beta-catenin,VEGFR-2 and SUFU have important effects on the pathogenesis and development of medulloblastoma.     

Relationship between Expression of beta-catenin and VEGFs （VEGFA, VEGF-C）, VEGF Receptors-2 （VEGFR-2） in Medulloblastoma

Objective： To investigate the expression of beta-catenin and VEGFs （VEGF-A, VEGF-C） and VEGF receptor-2 （VEGFR-2） protein in medulloblastoma. Methods： Immunohistochemical staining with SP method was conducted to determine the expression of beta-catenin and VEGFs （VEGF-A, VEGF-C） and VEGFR-2 in 33 cases of medulloblastoma and 10 normal cerebellar tissues. Results： The expression rate of beta-catenin, and VEGFs （VEGF-A, VEGF-C） and VEGFR-2 in medulloblastoma were significantly higher than that in normal tissue. A significant positive correlation was found between beta-catenin and VEGFs （VEGF-A, VEGF-C） and VEGFR-2 protein in medulloblastoma. Conclusion： There was a correlation between beta-catenin and VEGFs （VEGF-A, VEGF-C） and VEGFR-2 in medulloblastoma, which may play a role in the pathogenesis and development of medulloblastoma.     

Expressions of GSK-3beta, Beta-catenin and PPAR-gamma in medulloblastoma

Objective  To investigate the expressions of GSK-3beta, Beta-catenin and PPAR-gamma, and their relationship in medulloblastoma, and to explore their value in clinic application. Methods  Immunohistochemical staining with SP method was conducted to determine the expressions of GSK-3beta, Beta-catenin and PPAR-gamma in 48 cases of medulloblastoma and 10 normal cerebellar tissues. Results  The rate of abnormal expressions of beta-catenin and PPAR-gamma in MB was higher than that in normal. Conversely, GSK-3beta in MB was lower than that in the normal (P<0.05). Furthermore, in medulloblastoma, beta-catenin and GSK-3beta showed a negative correlation, PPAR-gamma and beta-catenin had a positive correlation. Conclusion  Abnormal expression of beta-catenin plays a crucial role in the development of medulloblastoma. Meanwhile, PPAR-gamma and GSK-3beta which are tightly related with beta-catenin are both involved in the genesis and development of medulloblastoma.      

胃癌中幽门螺杆菌感染与VEGF-C、VEGFR-3表达的关系

目的 探讨胃癌组织中血管内皮生长因子C（VEGF-C）、血管内皮生长因子受体3（VEGFR-3）的表达与幽门螺杆菌（Hp）感染及临床病理特征的关系。方法 采用13C呼气试验及改良Giemsa法检测40例胃癌患者的Hp感染情况,采用免疫组化SP法检测40例胃癌及配对癌旁组织中VEGF-C、VEGFR-3蛋白的表达。结果 胃癌患者Hp感染率为65.0％。VEGF-C、VEGFR-3蛋白在胃癌组织中的表达量分别为（8.74±0.84）×10-2和（3.64±1.96）×10-2,在癌旁组织中为（1.23±0.38）×10-2和（0.90±0.18）×10-2,差异均有统计学意义（P＜0.05）。VEGF-C与VEGFR-3蛋白表达呈正相关（r=0.51,P＜0.05）。VEGF-C、VEGFR-3蛋白的表达与肿瘤浸润深度、分化程度、淋巴结转移、TNM分期有关（P＜0.05）,与肿瘤大小无关（P＞0.05）。VEGF-C、VEGFR-3蛋白在Hp感染阳性胃癌组织中的表达量分别为（11.72±3.07）×10-2和（8.71±2.15）×10-2,在Hp感染阴性胃癌组织中分别为（8.48±1.11）×10-2、（5.37±1.05）×10-2,差异均有统计学意义（P＜0.05）。结论 VEGF-C、VEGFR-3表达与Hp感染和胃癌的发生、发展有关,且Hp感染可能与胃癌淋巴管生成有关。     

HO-1和Nrf-2在髓母细胞瘤中的表达和意义

  目的  髓母细胞瘤是儿童后颅凹常见恶性肿瘤, 本研究目的在于检测Nrf-2和HO-1在髓母细胞瘤中的表达, 并探讨其在髓母细胞瘤发生发展的意义。  方法  应用免疫组化SP法检测41例髓母细胞瘤及27例瘤旁对照脑组织中Nrf-2和HO-1的表达, 并结合研究病例的临床资料(患者性别、年龄、临床症状、肿瘤大小、肿瘤病理分型), 进行相关性分析。  结果  在髓母细胞瘤病例中, Nrf-2和HO-1的阳性表达率(分别为82.9%, 78.0%)与瘤旁对照组织的阳性表达率(分别为37.0%, 29.6%)相比明显升高, 差异具有显著统计学意义(P < 0.001), 并且二者之间呈显著正相关关系(P < 0.05)。但Nrf-2和HO-1的表达与分析的病例的临床特征无显著相关性(P > 0.05)。  结论  Nrf-2和HO-1的高表达可能在髓母细胞瘤的发生发展中发挥重要作用。      

HGF和VEGFR-3在大肠癌中的表达及其在淋巴管生成和转移中的作用

目的观察肝细胞生长因子(HGF)和血管内皮生长因子受体3（VEGFR-3）在大肠癌组织中的表达,检测大肠癌组织中的微淋巴管密度(LMVD),探讨HGF和VEGFR-3 在大肠癌淋巴管生成及转移中的作用。方法应用免疫组织化学（SABC）法检测52例大肠癌、20例大肠息肉组织和20例健康对照组织中HGF和VEGFR-3的表达,并应用podoplanin标记淋巴管,检测各组组织中的淋巴管密度,结合大肠癌患者的临床病理资料,分析其相关性。结果(1)大肠癌组织中HGF和VEGFR 3均被染成棕褐色或棕黄色,阳性表达率分别为75%、67.3%,相对表达量分别为(0.36±0.07)、(0.41±0.10),均明显高于大肠息肉组[25%、30%; (0.24±0.06)、 (0.28±0.03）] 和正常大肠组织[15%、10%;(0.23±0.06)、(0.21±0.03）]的表达(P<0.01)。且微淋巴管密度(LMVD)也明显增多[(4.13±1.99) vs. (2.59±1.46) vs. (2.40±1.44),P<0.01]。(2)大肠癌组织中HGF、VEGFR 3的相对表达量,LMVD三者间呈两两正相关。39例HGF表达阳性的大肠癌,其LMVD明显大于HGF表达阴性者[(4.25±2.13) vs. (2.73±1.54),t=3.051,P=0.003];35例VEGFR-3表达阳性的大肠癌,其LMVD也明显大于VEGFR 3表达阴性者[(3.79±1.26) vs. (2.64±1.32),t=3.235,P=0.002]。(3)HGF、VEGFR 3和LMVD的表达与大肠癌患者年龄、性别以及肿瘤分化程度无关(P>0.05),但与Dukes分期(P=0.034,P=0.021,P=0.006)、淋巴管有无转移明显相关(P=0.015,P=0.012,P=0.001)。结论HGF与大肠癌的淋巴管生成及转移有一定的相关性,并可能通过VEGF-C、D/VEGFR-3 信号途径间接促进淋巴管增生,从而促进肿瘤细胞的淋巴转移。     

结直肠腺癌中MMP-7的表达与淋巴管生成的关系

目的：通过检测结直肠癌中MMP-7、VEGF-D、VEGFR-3和D2-40的表达,探讨三者的关系以及MMP-7对淋巴管生成的作用。方法：应用免疫组化法检测124例结直肠癌和70例正常黏膜组织中MMP-7、VEGF-D、VEGFR-3和D2-40标记的淋巴管密度（MLVD）的表达,比较两种组织中蛋白表达的差异,分析其与结直肠癌临床病理指标的关系。结果：MMP-7、VEGF-D、VEGFR-3和MLVD的表达在结直肠癌中明显高于黏膜组织（P〈0.001）,MMP-7的表达与结直肠癌的肿瘤体积、Dukes分期、浸润深度及淋巴结转移有关（P〈0.05）;VEGF-D、VEGFR-3和MLVD的表达与肿瘤体积、淋巴结转移有关（P〈0.05）。结直肠癌中MMP-7和VEGF-D、VEGFR-3、MLVD的表达呈正相关性（均P=0.01）。而且MMP-7和VEGF-D、VEGFR-3、MLVD高表达与患者的不良预后相关（P〈0.05）。结论：结直肠癌中MMP-7的高表达可能与结直肠癌中的淋巴管生成有促进作用,联合检测MMP-7、VEGF-D、VEGFR-3 MLVD的表达对判断其预后有一定的指导意义。     

Expressions of beta-catenin, APC Protein, C-myc and Cyclin D1 in Ovarian Epithelial Tumor and Their Implication

Objective： To investigate the expressions of beta-catenin, protein APC （adenomatous polyposis coil protein）, c-myc and cyclin D1 and their implication in ovarian epithelial tumor. Methods： Immunohistochemical staining with SP method was conducted to identify the expressions of beta-catenin, APC protein, c-myc and cyclin D1 in ovarian epithelial tumor in 48 cases. Results： The abnormal expression rate of beta-catenin in malignant and borderline ovarian epithelial tumors was higher than that in benign epithelial tumors （P〈0.01）. The expression rates of c-myc and cyclin-D1 in ovarian malignant and borderline epithelial tumors were higher than those in benign epithelial tumors too（P〈0.05）. The prevalence of APC protein positive expression in benign epithelial tumors were significantly greater than that in malignant epithelial tumors （P〈0.05）. A significant negative correlation was found between beta-catenin and APC protein in ovarian epithelial tumors; while a significant positive correlation was found between beta-catenin, c-myc and cyclin-D1 in ovarian epithelial tumor （P〈0.05）. Conclusion： The abnormal expressions of Beta-catenin, APC protein, c-myc and cyclin-D1 might be used to indicate the malignance transform of ovarian epithelial tumors.     

Expression of Wnt-1, beta-catenin and c-myc in Ovarian Epithelial Tumor and Its Implication

Objective： To investigate the expression of Wnt-1, beta-catenin and c-myc in normal ovarian epithelial cell and malignant ovarian epithelial tumor. Methods： Immunohistochemical staining with SP method was conducted to identify the expression of Wnt-1, beta-catenin and c-myc in 18 samples of normal epithelial tissue and 34 cases of malignant epithelial tumor of ovary. Results： The expression rate of Wnt-1 and c-myc in malignant epithelial tumors was higher than those in normal epithelial cell （P〈0.05）. The abnormal expression rate of beta-catenin in malignant ovarian epithelial tumors was higher than that in normal epithelial cell （P〈0.05）. A significant positive correlation was found between Wnt-1, beta-catenin and c-myc in malignant ovarian epithelial tumor （P〈0.05）. A significant difference of expressions of Beta-catenin and C-myc was found between serous and mucinous tumors （P〈0.05）. Conclusion： The abnormal expression of Wnt-1, beta-catenin and c-myc might indicate the malignant transformation in ovarian epithelial tumors.     

VEGF-C和受体VEGFR-3 mRNA在食管鳞癌中的表达及意义

目的:观察血管内皮生长因子C(VEGF-C)及其受体血管内皮生长因子受体3(VEGFR-3)在食管鳞癌组织中的表达,分析其与肿瘤淋巴结转移的关系.方法:采用RT-PCR方法检测32例食管鳞癌组织、10例正常食管粘膜中VEGF-C/VEGFR-3 mRNA的表达情况,分析其与各临床病理因素之间的关系.结果:食管鳞癌组织中VEGF-CmRNA表达阳性率为68.75%(22/32),其中18例VEGFR-3 mRNA阳性,VEGF-C和VEGFR-3的表达密切相关(P＜0.01).VEGF-C mRNA的表达与食管鳞癌的淋巴结转移、浸润深度显著相关(P＜0.01,P＜0.05),但与患者年龄、肿瘤大小及分化程度无关.正常食管粘膜中未见VEGF-C/VEGFR-3 mRNA表达.结论:VEGF-C/VEGFR-3是促进食管鳞癌经淋巴结转移的重要因素.     

血管内皮生长因子受体3与PI3K/AKT在胃癌组织中的表达及意义

 目的 观察血管内皮生长因子受体3（VEGFR-3）和磷脂酰肌醇-3-激酶(PI3K)、蛋白激酶B (PKB或Akt)在胃癌组织及相应癌旁组织中的表达情况。 方法 采用RT-PCR法检测36例胃癌及相应癌旁对照组织中VEGFR-3、PI3K、Akt mRNA 的表达;免疫组织化学法检测VEGFR-3、P-Akt蛋白的表达,并用图像分析方法测定平均吸光度值。 结果 1. RT-PCR 胃癌组织中VEGFR-3mRNA、PI3KmRNA、AktmRNA与癌旁对照组织的表达水平相比,差异有统计学意义(P<0.05),且VEGFR-3mRNA与PI3KmRNA、AktmRNA表达呈正相关 (P<0.05)。VEGFR-3 mRNA表达与TNM分期、浸润深度、淋巴结转移与远处转移有关(P<0.05)。2. 免疫组织化学 VEGFR-3、P-Akt蛋白在胃癌组织中的表达水平明显高于癌旁对照组织(P<0.05),且VEGFR-3、P-Akt蛋白表达水平呈正相关(P<0.05)。 结论 VEGFR-3阳性表达的胃癌患者可能更易发生淋巴结转移,VEGFR-3可作为评估患者预后的重要指标。VEGFR-3与PI3K/Akt的表达具有一定的相关性。     

非小细胞肺癌组织中VEGF-C及其受体VEGFR-3的表达与淋巴结转移的关系

 目的　研究血管内皮生长因子C（VEGF-C）及VEGF受体-3（VEGFR-3）在人类非小细胞肺癌（NSCLC）组织中的表达,并探讨其与淋巴转移之间的关系。方法　对60例NSCLC患者术后标本行VEGF-C、VEGFR-3免疫组织化学（SP法）检测,计数阳性率,并结合临床和病理资料进行分析。结果　60例NSCLC组织中VEGF-C阳性表达率为68.3 %（41／60）,VEGFR-3阳性表达率为53.3 %（32／60）,VEGF-C的表达与肺癌分化程度呈负相关（P＝0.004）,VEGF-C、VEGFR-3的表达与淋巴结转移呈正相关（P＝0.009）,肺癌组织中VEGF-C与VEGFR-3的表达亦相关（r＝0.27）。结论　NSCLC组织中VEGF-C、VEGFR-3的表达与肿瘤细胞的淋巴结转移相关。VEGF-C是促使肿瘤组织内淋巴管形成,促进肺癌淋巴结转移的重要原因。     

High co-expression of vascular endothelial growth factor receptor-1 and Snail is associated with poor prognosis after curative resection of hepatocellular carcinoma

VEGFR-1-mediated signaling promotes invasiveness by direct tumor activation in some cancers. However, VEGFR-1 expression and its relationship with clinical features and prognosis in HCC remained unclear. Overexpression of Snail is common in HCC and associated with poorer prognosis. Therefore, expression of VEGFR-1 and Snail was investigated in HCC cell lines and tissue specimens in our study, and special attention was paid to evaluating the role of VEGFR-1 expression in prognosis of HCC. Western blot and immunofluorescence were used to detect expression of VEGFR-1, Snail and MMP-9 in 4 HCC cell lines, respectively. Moreover, expression of these proteins was confirmed on the samples from 95 HCC patients who underwent curative resection using immunohistochemistry. ROC and survival analysis determined the predictive values of parameters and the association with survival. HCC cell lines with a higher VEGFR-1 expression were more invasive. Both VEGFR-1 and Snail expressions were significantly higher in HCC tissues than in non-cancerous tissues. High-expression VEGFR-1 and Snail, associated with adverse clinical features, were independent prognostic factors for RFS and OS (P?=?0.023 and P?=?0.044, respectively). Positive correlation was found between VEGFR-1 and Snail or MMP-9 (r?=?0.418 and r?=?0.232, respectively, P?相似文献   

卵巢浆液性肿瘤组织中hk10基因的表达

 目的 研究卵巢癌组织中人激肽释放酶10（hk10）的表达,探讨hk10在卵巢癌诊断中的意义。 方法 （1）应用RT-PCR检测10例卵巢浆液性囊腺癌组织、10例卵巢浆液性囊腺瘤组织及10例正常卵巢组织中hk10 mRNA的表达。（2）应用免疫组织化学法检测60例卵巢浆液性囊腺癌、24例卵巢浆液性囊腺瘤及24例正常卵巢组织中hk10蛋白的表达。 结果 （1）hk10 mRNA在卵巢癌组的表达明显高于正常卵巢和卵巢良性肿瘤组（P＜0.01)。而卵巢良性肿瘤组和正常卵巢组的表达差异无统计学意义（P＞0.05）。（2）hk10蛋白在卵巢癌组的阳性表达率明显高于正常卵巢组和卵巢良性肿瘤组（P＜0.01);随着卵巢癌病理级别的增加hk10的蛋白表达增强（P＜0.01);Ⅱ期和Ⅳ期卵巢癌hk10蛋白阳性表达率高于Ⅰ期卵巢癌。 结论 hk10 mRNA及蛋白在卵巢癌组织中表达显著增高,在卵巢癌组织中hk10蛋白表达程度与病理级别及临床分期相关,hk10基因在卵巢癌早期诊断研究中意义重大。     

The effect of the expression of vascular endothelial growth factor (VEGF)-C and VEGF receptor-3 on the clinical outcome in patients with gastric carcinoma

Aims

We aimed to investigate the relationship among VEGF-C/VEGFR-3 expression, lymphatic metastasis and patient prognosis in gastric carcinoma.

Material and methods

VEGF-C and VEGFR-3 expression in gastric carcinoma tissues obtained from 204 patients who underwent curative gastrectomy (105 cases presented with lymph node metastasis and 99 cases without metastasis) was examined immunohistochemically. There was no significant difference in the other clinicopathologic variables except for postoperative pathological tumor stage (pT) and TNM stage between the two groups. The results were statistically processed.

Results

The results showed that VEGF-C was located mainly in the cytoplasm of tumor cells and VEGFR-3 was found predominantly in the endothelium of lymphatic vessels. VEGF-C and VEGFR-3 expression was more frequent in gastric carcinoma tissues than that in normal gastric tissues, 54.90% and 35.29% respectively, which revealed that the expression of VEGF-C and VEGFR-3 was significantly stronger in patients with lymph node metastasis than in those without metastasis. Patients who had positive staining for VEGF-C showed significantly less favorable survival rates compared with patients who had negative staining for VEGF-C. The survival rates of patients who had positive staining for VEGFR-3 also were significantly lower compared with patients who had negative staining for VEGFR-3. Patients who had positive staining for both VEGF-C and VEGFR-3 exhibited the most unfavorable prognosis. Multivariate analysis demonstrated that the expression of VEGF-C and VEGFR-3 was an independent prognostic determinant. In addition, faint to moderate VEGF-C expression was detected in normal gastric epithelial cells (18/204, 8.9%).

Conclusions

VEGF-C and VEGFR-3 expression could serve as a prognostic biomarker in patients with gastric carcinoma.     

肺腺癌组织中MACC1和c-Met蛋白的表达及其与患者术后复发的关系

目的 检测MACC1、c-Met蛋白在肺腺癌组织中的表达,及其在患者术后复发中的预测价值。方法 收集完全性切除的肺腺癌患者术后病理标本102例和癌旁组织57例,采用免疫组织化学技术检测MACC1和c-Met蛋白在肺腺癌和癌旁组织中的表达,对患者行定期随访,分析MACC1和c-Met 蛋白的表达与患者术后复发的关系。结果 MACC1和c-Met在癌组织中的阳性表达率分别为59.80%、54.90%,明显高于癌旁组织中的7.01%、10.53%,差异有统计学意义（P<0.05）。MACC1、c-Met蛋白的表达与性别年龄无关,与肿瘤T分期、淋巴结转移、病理TNM分期有关（P<0.05）。MACC1与c-Met 的表达之间呈正相关（r=0.262, P=0.008）。MACC1阳性组2年内复发率为73.77%,明显高于阴性组的31.71%（χ²=17.686, P<0.001）。c-Met阳性组2年内复发率为76.79%,高于阴性组的34.78%（χ²=18.272, P<0.001）。Cox回归多因素提示MACC1和c-Met蛋白的表达、术后病理分期、肿瘤T分期及淋巴结转移情况是腺癌患者复发转移的危险因素。结论 MACC1和c-Met蛋白在肺腺癌组织中表达与肿瘤T分期、淋巴结转移、病理分期有关,影响肺腺癌患者术后无瘤生存期,是复发转移的危险因素。     

大肠癌组织中p73、p51的相关性研究

目的:研究大肠癌组织中p73,p51基因的表达情况及其临床意义。方法:采用逆转录聚合酶链反应技术(RT-PCR),检测30例大肠癌组织及邻近正常大肠粘膜组织p73及p51的两个转录p51A、p51BmRNA表达情况。结果:30例大肠癌组织中有22例p73表达阳性,相对应的正常大肠组织仅有3例表达阳性,两者具有显著性差异(P<0.01),大肠癌组织的p73阳性表达率与大肠癌的分化程度及TNM分期均无关(P>0.05);p51A、p51B在30例大肠癌组织和相对应的正常大肠组织呈低水平表达,其中大肠癌组织中p51A表达量明显高于正常大肠组织(P<0.01),与细胞分化程度及TNM分期无关(P>0.05),而p51B在大肠癌组织和正常大肠组织中的表达量无明显差异(P>0.05)。结论:大肠癌组织中存在p73、p51A的过度转录表达,p73、p51A的过度表达与大肠癌的发生发展存在一定的关系。     

错配修复基因hMLH1和hMSH2在散发性大肠癌中的表达及其意义

目的 探讨错配修复基因hMLH1和hMSH2在散发性大肠癌(SCC)组织中的表达及其临床意义.方法 采用免疫组化Max Vision二步法对63例SCC标本中的癌组织、距癌灶3 cm以外的癌旁组织、距癌灶10 cm以外的正常组织中hMLH1和hMSH2蛋白的表达进行检测.结果 hMLH1蛋白在63例正常大肠组织、癌旁组织和SCC组织中的阳性表达率分别为95.2%、85.7%和81.0%,hMLH1蛋白在SCC组织中的阳性表达率明显低于正常大肠组织(P＜0.05).hMSH2蛋白在63例正常大肠组织、癌旁组织和SCC组织中的阳性表达率分别为76.2%、66.7%和52.4%,hMSH2蛋白在SCC组织中的阳性表达率明显低于正常大肠组织(P＜0.01).hMLH1蛋白在＜60岁的SCC患者组织中的阳性表达率(100%)明显高于≥60岁的SCC患者组织(75.0%,P＜0.05),在有淋巴结转移的SCC组织中的阳性表达率(50.0%)明显低于无淋巴结转移的SCC组织(93.3%,P＜0.05).hMSH2蛋白在＜60岁的SCC患者组织中的阳性表达率(80.0%)明显高于≥60岁的SCC患者组织(43.8%,P＜0.05),在癌组织浸润至肠壁浆膜层SCC组织中的阳性表达率(61.5%)明显高于浸润至黏膜下层和肌层的SCC组织(37.5%,P＜0.05).SCC组织中hMLH1和hMSH2蛋白的表达呈正相关关系(r=0.254,P＜0.01).结论 hMLH1和hMSH2蛋白在SCC组织中的表达均有一定的缺失,并且与患者的年龄、淋巴结转移和癌组织浸润的范围有关.hMLH1和hMSH2基因可以作为临床预测和判断SCC发生和发展有用的实验室指标.     

β-Catenin,cyclinD1及c-myc在肝细胞癌中的表达

目的探讨β-Catenin、cyclinD1及c-myc蛋白的表达与肝癌临床病理参数的关系。方法采用EnVisionTMplus免疫组织化学方法研究β-Catenin、cyclinD1及c-myc蛋白在52例HCC及癌旁肝组织中的表达情况。结果52例HCC中β-Catenin、cyclinD1及c-myc蛋白染色阳性率分别为55.8%、48.1%及53.8%,癌旁肝组织的阳性率为36.5%、25.0%及32.7%,β-Catenin、cyclinD1及c-myc在HCC及癌旁肝组织两者间有显着性差异(P<0.05)。在HCC中β-Catenin的阳性表达与cyclinD1、c-myc的阳性表达密切相关(P=0.0108,r=0.3920;P=0.0295,r=0.3406)且呈正相关;β-Catenin、cyclinD1及c-myc在人肝癌组织中的检出率与肝外转移、术后复发及肿瘤分化程度明显有关(P<0.05),cyclinD1的检出率与门静脉癌栓也明显有关(P<0.05),β-Catenin的检出率与临床分期和门静脉癌栓也有显著性差异(P<0.05)。结论β-Catenin、cyclinD1及c-myc蛋白...