共查询到19条相似文献,搜索用时 203 毫秒
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《沈阳药科大学学报》2016,(5):391-395
目的研究苦荞麦总黄酮对软脂酸损伤脐静脉内皮细胞的保护作用。方法体外培养人脐静脉内皮细胞EA.hy926,将细胞培养液分为正常对照组、模型组、苦荞麦总黄酮低浓度组、苦荞麦总黄酮中浓度组、苦荞麦总黄酮高浓度组和二甲双胍组。用软脂酸建立胰岛素抵抗状态下的血管内皮细胞模型,MTT法检测细胞增殖率,LDH试剂盒检测细胞LDH的漏出量,流式细胞技术检测细胞的凋亡率。结果与正常组比较,模型组细胞增殖率明显降低(P<0.01),LDH漏出量显著增高(P<0.01),凋亡率明显增高(P<0.01)。同模型组相比,苦荞麦总黄酮低、中、高浓度组,以及二甲双胍组细胞增殖率明显增高(P<0.01),凋亡率显著降低(P<0.01),且苦荞麦总黄酮各浓度组表现出明显的剂量依赖关系;苦荞麦中、高浓度组,以及二甲双胍组LDH漏出量明显减少(P<0.01)。结论苦荞麦总黄酮对软脂酸损伤的脐静脉内皮细胞具有明显的保护作用。 相似文献
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《实用口腔医学杂志》2018,(2)
<正>苦荞麦[Fagopyrum tataricum(L.)Gaertn]为蓼科荞麦属植物,在中国资源极其丰富,主要分布在贵州、四川、云南、陕西、山西和内蒙古等地。苦荞属于药食两用植物,具有较高的营养保健和医药学价值。苦荞麦中含有丰富的活性蛋白,其具有降血糖、降血脂、抗氧化、抗肿瘤等药理作用[1]。左光明等[2]以苦荞二道荞麸为原料,在苦荞蛋白质Osborne分级分离的基础上,对各蛋白组分进 相似文献
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目的用差热分析法(DTA)和近红外漫反射光谱(NIRS)法对11种栽培苦荞麦进行快速分析鉴别。方法应用TG-DTA热重差热联用仪分别对11个栽培品种苦荞麦进行差热图谱扫描与分析。实验条件为差热量程:±50μV;升温速率:15℃/min;升温范围:35℃~500℃;气氛为氮气,流速:50 ml/min。应用Bruker Vector 22/N型傅立叶变换近红外光谱仪对11种栽培苦荞麦进行扫描。实验条件为测定方式:积分球漫反射;扫描次数64次;扫描范围:3 700~12 000/cm。结果结合两种方法能快速鉴别"建德苦荞麦"同其他苦荞麦,鉴别效果良好。结论 "建德苦荞麦"作为一个新的培育品种,可以对其营养成分、药理活性进行进一步评价,为广泛推广提供参考。 相似文献
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苦荞降糖胶囊的致突变性研究 总被引:2,自引:0,他引:2
目的研究苦荞降糖胶囊的致突变性。方法分别采用Ames试验、小鼠骨髓嗜多染红细胞微核试验和小鼠精子畸形试验对基因水平和细胞水平的遗传损伤进行检测。结果该胶囊在0.1、0.5、1.0、2.0和5.0mg/皿的剂量下对TA97、TA98、TA100和TA102四株试验菌株均未引起自发回变菌落数增加;在1250和5000mg/kg体重剂量下均未引起小鼠骨髓嗜多染红细胞微核率和小鼠精子畸形率升高(字2检验,P>0.05)。结论该胶囊在基因水平和细胞水平均不具有致突变性。 相似文献
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苦荞麦种子的化学成分 总被引:5,自引:1,他引:4
目的对苦荞麦〔Fagopyrum tataricum(L.)Gaertn.〕的种子中的化学成分进行研究。方法采用硅胶柱色谱等方法进行分离纯化,根据理化性质和光谱数据进行结构鉴定。结果分离得到11个化合物,分别鉴定为乌苏酸(ursolic acid,1)、山柰酚(kaempferol,2)、槲皮素(quercetin,3)、大黄素(emodin,4)、山柰酚-3-O-芸香糖苷(kaempferol-3-O-rutinoside,5)、芦丁(rutin,6)、3′,5′-二甲氧基-4′-O-β-D-吡喃葡萄糖基桂皮酸(3′,5′-dimethoxy-4′-O-β-D-glucopyranosyl-cinnamic-acid,7)、β-谷甾醇(β-sitosterol,8)、胡萝卜苷(daucosterol,9)、蔗糖(sucrose,10)、果糖(fructose,11)。结论化合物7是从荞麦属中首次分离得到,化合物1是从该植物中首次分离得到。 相似文献
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1 Intracerebroventricular injection of prostanglandin F2alpha (10-40 microgram) decreases food intake in a dose-dependent manner in rats trained to consume their daily total food intake in a 2 h period. 2 This anorexia is also observed in satiated rats, which had ad libitum access to food. 3 The anorectic activity of prostaglandin F2alpha is not modified by changes in the internal environment of the body after food intake, such as increased blood glucose and insulin levels and decreased fatty acid levels, or by the presence or absence of food in the stomach, as is evident from the anorectic activity of prostaglandin F2alpha in partially satiated rats. 4 The anorexia is not due to pain or irritative properties of prostaglandin F2alpha since induction of comparable pain with 3% acetic acid does not affect food intake in rats deprived of food for 22 hours. 5 Anorectic doses of prostaglandin F2alpha when injected intraperitoneally cause hypothermia. 6 The results suggest that the inhibitory activity of prostaglandin F2alpha on food intake is at both peripheral and central sites. 7 Prostaglandin F2alpha-induced anorexia is associated with the behavioural tranquilization that is seen after the ingestion of food. 相似文献
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Chicken nucleated blood cells as a cellular model for genotoxicity testing using the comet assay. 总被引:2,自引:0,他引:2
Mycotoxins can frequently occur in animal feed and human food. T-2 toxin, as the most toxic trichothecene, has been implicated as the causative agent in a variety of animal diseases and is associated with some human diseases. The comet assay was performed as a test for detection of DNA damage caused by T-2 toxin in peripheral blood cells of chicken. The suitability of the comet assay as a biomarker for genotoxic analysis has been applied in studies using human white blood cells. It can be applied to any tissue from which a single cell suspension can be obtained. The method has already been applied to chicken as a foodstuff for detection of irradiation of food containing DNA. However, application of the method on chicken blood cells has not been set up yet. The aim of this research was to develop a protocol for detection of DNA damage induced by T-2 toxin in chicken blood cells. Chickens were administered orally with T-2 toxin and the samples of whole blood were collected at 24 h post treatment. The DNA damage was determined by an increase in the comet parameters in tested animals. Our results show that T-2 toxin had induced significant DNA damage in treated chicken as compared with control animals, indicating that the assay can be used for the assessment of primary DNA damage caused by mycotoxins. 相似文献
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金荞麦的化学成分研究 总被引:11,自引:0,他引:11
目的研究中药金荞麦中的化学成分。方法运用多种色谱方法分离化学成分 ,依据理化性质、波谱 (NMR、EI- MS)数据分析鉴定化合物结构。结果从金荞麦中初步分离得到 5个化合物 ,分别鉴定为赤杨酮 (glutinone ,Ⅰ )、赤杨醇 (glutinol,Ⅱ )、棕榈酸单甘油酯 (glycerolmonopalmitate ,Ⅲ )、木犀草素 (luteolin ,Ⅳ )、正丁醇 - β- D- 吡喃型果糖苷 (n butyl -β- D fructopyronoside ,Ⅴ )。 结论 5个化合物均为首次从荞麦属植物中分离得到。 相似文献
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J Fonseca de Oliveira M B Nunes de Oliveira A S Avila A C S Braga M T Jansen de Almeida Catanho R L Cavalcanti Jales V N Cardoso M Bernado-Filho 《Food and chemical toxicology》2003,41(1):15-20
Natural products are widely used as food or food additives or medicines for humans. We are trying to develop a model to assess the possible toxic properties of natural products, such as Fucus vesiculosus, utilized in popular medicine. Red blood cells (RBC) labeled with technetium-99m (99mTc) are used in various procedures in nuclear medicine. This labeling procedure depends on a reducing agent, and stannous chloride is used. There is evidence that this labeling may be altered by drugs. We have investigated the possibility that F. vesiculosus extract is capable of altering the labeling of blood elements with 99mTc. Blood was incubated with F. vesiculosus extract and stannous chloride solution and Tc-99m added. Blood was centrifuged and plasma (P) and blood cells (BC) were isolated. Samples of P or BC were also precipitated, centrifuged and insoluble (IF) and soluble (SF) were separated. The percentages of radioativity (%ATI) in BC, IF-P and IF-BC were calculated. Histological preparations of the RBC treated with F. vesiculosus revealed that this extract is capable of promoting important modifications on the shape of the RBC. The%ATI decreased on BC from 93.6+/-2.3 to 29.0+/-2.7, on IF-P from 77.6+/-1.2 to 7.5+/-1.0 and on IF-BC from 80.0+/-3.4 to 12.6+/-4.8. Once the RBC labeling procedure with 99mTc depends on the presence of stannous (+2) ions, the substances present in the F. vesiculosus extract should increase the valence of these ions to stannic (+4). This would decrease the%ATI on blood elements and indicate the presence of oxidant agents in the F. vesiculosus extract. 相似文献
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目的 该研究为进一步开发利用荞麦及贵州毕节特产荞酥的品质研究提供理论依据。方法 采用顶空固相微萃取-气质联用技术(HS-SPME-GC-MS)采集并鉴定荞麦加工前后的风味成分,并用峰面积归一法测定各成分相对含量。结果 从加工前后的荞麦总离子流图中分别鉴定出 52、41种化合物。结论 荞麦的主要风味成分均为醛类、醇类、烷烃类,其中含量最高的挥发性化合物均为己醛、壬醛、癸醛、2,2,4,6,6-五甲基庚烷,加工前后荞麦风味造成主要差异的成分是醇类物质。 相似文献
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Vuotto ML Basile A Moscatiello V De Sole P Castaldo-Cobianchi R Laghi E Ielpo MT 《International journal of antimicrobial agents》2000,13(3):197-201
The present study was designed to evaluate the antibacterial and antioxidant activities of an aqueous extract from the tropical Feijoa sellowiana Berg. fruit which is widely used for human food. The extract was tested against gram-positive and gram-negative bacteria by a broth dilution test and on human whole blood leukocytes, as well as isolated neutrophils using a chemiluminescence (CL) assay. The extract inhibited bacterial growth; Pseudomonas aeruginosa, Enterobacter aerogenes and Enterobacter cloacae were the most sensitive. The fruit extract significantly decreased CL emission from human whole blood phagocytes and isolated polymorphonuclear leukocytes whether they were activated or not by soluble or phagocytic stimuli. F. sellowiana showed both antibacterial and antioxidant properties and therefore its extract might be used as a new multifaceted drug. 相似文献