MGIT 及BacT/Alert MP液体培养基检测分枝杆菌效果的评价

目的 评价 BacT/Alert MP、MGIT 960 7 mL、MGIT 4 mL 3种液体培养基检测分枝杆菌的效果.方法收集肺结核病疑似患者的149份抗酸染色涂阳、188份涂阴痰标本,分别采用BacT/Alert MP、MGIT 960 7 mL、MGIT 4 mL以及罗氏培养基(L-J)进行分离培养效果比对.另...     

两种方法在结核分枝杆菌培养中的比对研究

目的比较MGIT960系统液体培养法与改良罗氏(L-J)培养法在结核分枝杆菌培养中的效果。方法选择2015年1月至2017年12月到该所就诊的活动性肺结核患者3 685例,对每份病例标本分别进行痰涂片抗酸染色(萋-尼法)镜检、改良罗氏(L-J)培养法和MGIT960系统液体培养法,并分别计算抗酸染色镜检与改良罗氏(L-J)培养法和MGIT960系统液体培养法的阳性符合率;统计改良罗氏(L-J)培养法和MGIT960系统液体培养法的阳性率、污染率及培养时间。结果痰涂片镜检与改良罗氏(L-J)培养法和MGIT960系统液体培养法的阳性符合率分别为94.21%(114/121)和82.64%(100/121)。改良罗氏(L-J)培养法和MGIT960系统液体培养法结果符合率为93.70%(3 197/3 412),所需的培养时间分别为28.0(25.0～31.0)d和16.5(14.0～19.0)d。结论 MGIT960系统液体培养法阳性率与传统的改良罗氏(L-J)培养法基本一致,报告时间平均缩短至16.5d,比改良罗氏(L-J)培养法提前10.0d以上,为临床早诊断、早治疗提供了可靠依据,而且为后续进行的药敏试验提供了菌株。     

MGIT液体培养基检测分枝杆菌效果的评价

目的 应用荧光判读仪手工判读方法对MGIT液体培养基进行快速检测分枝杆菌的效果评价.方法 收集2008年10月至2009年1月上海市各区结核病定点医院初诊患者首次痰标本200份,其中痰涂片法抗酸染色阳性标本67份,阴性标本133份.分别对L-J培养基、BacT/Alert 3D 系统和MGIT液体培养基分枝杆菌分离培养效果比对.结果 200份痰标本中,共分离培养出105株分枝杆菌菌株,检出率为52.5%;MGIT液体培养基检出率为49.5%(99/200),BacT/Alert 3D系统检出率为48.0%(96/200),L-J培养基检出率为45.0%(90/200).MGIT液体培养基分离培养检出率高于L-J培养方法,差异有统计学意义(x2=5.40,P=0.020 1).在133份抗酸染色阴性标本中,用MGIT液体培养基、BacT/Alert 3D系统和L-J培养基分离培养检出率分别为24.8%(33/133)、23.3%(31/133)、18.8%(25/133).MGIT液体培养基和L-J培养基分离培养检出率差异有统计学意义(x2=5.33,P=0.020 9).MGIT液体培养基分离培养阳性报告时间中位数为11 d;BacT/Alert 3D系统分离培养阳性报告时间中位数为15 d;L-J培养基分离培养阳性报告时间中位数为22 d.MGIT液体培养基阳性报告时间短于BacT/Alert 3D系统,差异有统计学意义(Z=3.414,P＜0.01);MGIT液体培养基阳性报告时间短于L-J培养基培养,差异有统计学意义(Z=7.083,P＜0.01).结论 MGIT液体培养基能快速检测分枝杆菌,检出率高,阳性报告时间短,且不需要昂贵仪器,成本低,适合基层实验室推广应用.

Abstract：

Objective To evaluate the effectiveness of MGIT liquid medium fluorescence instrument manual interpretation method for rapid detection of Mycobacterium. Methods Two hundred sputa with newly diagnosed tuberculosis patients were collected from October 2008 to January 2009 in the district hospitals in Shanghai. Of these 200 sputa, 67 sputa were positive AFB, 133 were negative. All the sputa were isolated by L-J, BacT / Alert 3D system and MGIT liquid medium methods. Results Of the 200 sputa specimens,105(52. 5% ) were isolated as Mycobacterium strains. The positive culture rate of the MGIT, BacT/Alert 3D and L-J method was 49. 5% ( 99/200 ), 48. 0% (96/200) and 45.0% ( 90/200), respectively. The MGIT culture positive rate was significantly higher than that of L-J method (x2 = 5.40, P = 0. 020 1 ). Of the 133 sputa with negative AFB, the positive culture rate was 24. 8% ( 33/133 ), 23. 3% ( 31/133 ) and 18. 8% (25/133) with MGIT, BacT/Alert 3D and L-J method, respectively. The MGIT culture positive rate with the AFB negative sputum was significantly higher than that of L-J method (x2 = 5. 33, P = 0. 020 9 ).The median time of detection with MGIT, BacT/Alert 3D system and L-J method was 11 days, 15 days and 22 days, respectively. Comparing the median time of detection of MGIT with BacT/Alert 3D, the difference was statistically significant ( Z = 3.414 ,P ＜ 0. 01 ). Comparing the median time of detection of MGIT with L-J method, the difference was statistically significant (Z =7.083,P＜0. 01).Conclusions MGIT liquid medium manual method is a rapid detection method of Mycobacterium with a high positive detection rate, and do not need expensive equipment This method may suitable to resource limited medical institutions due to its low cost and short round time.     

Xpert MTB/RIF检测利福平耐药的准确性与结核病患者痰细菌载量的相关性：一项天津市的回顾性研究

目的 分析Xpert MTB/RIF不同细菌载量(1+、2+、3+、4+)检测结核分枝杆菌利福平(RIF)耐药的准确性,为有效临床药敏结果判断提供参考。方法 回顾性收集2020年1月至2021年12月天津市结核病控制中心参比室接收的1 936份结核病疑似患者痰标本的液体BACTEC MGIT960培养阳性检测结果,排除16份非结核分枝杆菌后,1 158份XpertMTB/RIF阳性标本的结果纳入分析,对Xpert MTB/RIF检测阳性且液体BACTEC MGIT960培养阳性的1 142例结核分枝杆菌(MTB)菌株进行常规培养法体外药物表型药敏试验(DST)。应用SPSS 19.0软件进行统计学分析,结果采用配对χ²检验,P<0.05为差异有统计学意义;两种检验方法的一致性采用Kappa检验。结果 1 936份液体BACTEC MGIT960培养阳性标本中XpertMTB/RIF检测结核的阳性筛查率为59.81%,XpertMTB/RIF检测结核的细菌载量1+～4+中,RIF耐药的检出率分别为8.88%、14.91%、11.37%、15.15%。以表型DS...     

二代线性探针技术在结核病诊断及耐药检测中的应用研究

目的 探讨二代线性探针技术(MTBDR plus V2.0)在结核病及其耐药性诊断中的应用价值.方法 选取2018年6月至2019年6月于该院就诊的1322例疑似肺结核患者为研究对象,所有研究对象均用同一份痰标本同时进行痰涂片检测、MGIT 960液体培养和MTBDR plus V2.0基因检测.对培养阳性且鉴定为结核分枝杆菌的菌株进行MGIT 960药敏试验.采用Kappa检验比较药敏试验结果及效能.结果 以MGIT 960液体培养结果为标准,痰涂片和MTBDR plus V2.0检测结核分枝杆菌的灵敏度分别为46.74％和87.15％,特异度分别为98.73％和92.74％,Kappa值分别为0.45和0.80;MGIT 960液体培养和MTBDR plus V2.0对痰涂片阴性肺结核患者检测效能差异无统计学意义(χ2=0.071,P=0.790);MGIT 960和MTB-DR plus V2.0对利福平和异烟肼耐药性检测,Kappa值分别为0.84和0.69.结论 MTBDR plus V2.0能够快速对结核病进行诊断,且效能与MGIT 960液体培养相似,高于痰涂片检测.在利福平耐药检测方面,MTB-DR plus V2.0和MGIT 960有较好的一致性,但对异烟肼耐药性检测一致性一般.     

实时荧光PCR方法与痰涂片法检测结核杆菌的价值分析

目的比较实时荧光PCR法和痰涂片法检测结核杆菌的价值。方法选取2017年1月至2019年2月某中心结核门诊接诊的疑似肺结核患者95例,采集晨痰标本,分别予以痰涂片检测、PCR检测、MGIT960液体分离培养,观察比较三种检测方法的结核杆菌阳性检出率,以MGIT960液体分离培养法为金标准,对比实时荧光PCR法和痰涂片法的检测准确性、敏感度、特异度以及与MGIT960液体分离培养法检测结果的一致性。结果痰涂片法结核杆菌阳性检出率为29.47%(28/95),实时荧光PCR法检出率为37.89%(36/95),MGIT960液体分离培养法检出率为38.95%(37/95),三种检测方法结核杆菌阳性检出率比较差异无统计学意义(χ~2=2.239,P=0.3260.05)。痰涂片法检测结果与MGIT960液体分离培养法结果对比差异无统计学意义(P0.05),与MGIT960液体分离培养法结果一致性中等(K=0.467)。实时荧光PCR法检测结果与MGIT960液体分离培养法结果对比差异无统计学意义(P0.05),与MGIT960液体分离培养法结果一致性较好(K=0.933)。实时荧光PCR法检测准确率、敏感度均高于痰涂片法,对比差异有统计学意义(P 0.05),但两种检测方法特异度比较差异无统计学意义(P0.05)。结论实时荧光PCR法检测结核杆菌,能够提升结核杆菌阳性检出率,检测准确率、敏感度较高,与MGIT960液体分离培养法检测结果一致性较好,应用价值优于痰涂片检测。     

手工MGIT液体培养结核分枝杆菌污染再处理分析

目的分析手工分枝杆菌培养管(MGIT)液体培养法检测结核分枝杆菌中的污染再处理效果。方法收集疑似新发肺结核初诊的1 088例患者的痰标本,所有标本均分别用液体MGIT法、固体改良罗氏培养基L-J法及痰涂片抗酸染色进行检测,统计MGIT法首次培养总污染率和去污染后再次培养的总污染率,并和固体L-J法污染率进行比较。结果 MGIT法的首次污染率涂片阳性(简称涂阳)组为11.24%,涂片阴性(简称涂阴)组为7.29%,总污染率为7.90%;L-J法涂阳组的污染率为2.96%,涂阴组为3.80%,总污染率为3.68%,两者之间差异有统计学意义(P均0.05)。去污染后涂阳组的再污染率为0.59%,涂阴组为4.68%,总污染率为4.04%,与L-J法相应各组间差异无统计学意义(P均0.05);去污染后再培养涂阳组污染率低于涂阴组(P0.05)。结论 MGIT法可去污染再培养,以降低污染率,特别是涂阴标本应调整和增加前处理的消化时间或灭菌剂的浓度,以控制首次MGIT法的污染率,减少再处理去污染次数。建议临床医生应在控制其他细菌感染后再留标本做结核分枝杆菌培养。     

一种快速培养分枝杆菌生长指示(MGIT)系统应用评价

目的 观察比较分枝杆菌生长指示管(MGIT)法，固体罗氏(L-J)法，抗酸染色法对分枝杆菌的检出率及培养时间，应用一种快速、准确、阳性率高的分枝杆菌培养方法，及时给临床提供诊治依据。方法 采集住院和门诊疑似或确诊结核病患者的痰、支气管灌洗液、胸腹水等70份标本。用N-乙酰半胱氨酸-氢氧化钠(NALC-NaOH)对标本去污处理后接种MGIT和L-J培养基，同时作涂片抗酸染色。结果 MGIT法、L-J法、涂片法对分枝杆菌的检出率分别为67．1％、34．3％、12．9％，MGIT法和L-J法培养分枝杆菌的平均时间分别为14天、25天。结论 MGIT法培养分枝杆菌时间最短，检出率高于L-J和涂片法。     

GeneXpert MTB/RIF、MGIT960液体培养法在胸腔积液结核分枝杆菌检测中的应用

目的探讨GeneXpert MTB/RIF、MGIT960液体培养法在胸腔积液结核分枝杆菌检测中的应用。方法回顾性分析386例疑似和确诊结核病患者胸腔积液标本中腺苷脱氨酶(ADA)、GeneXpert MTB/RIF及MGIT960液体培养法的检测结果。以胸腔积液ADA>40 U/L作为鉴别结核性积液的标准,将标本分为3组:第1组为非结核性积液(ADA≤40 U/L,219例),第2组为结核性积液(ADA>40 U/L,145例),第3组为脓性胸腔积液(不能进行ADA检测,22例)。结果在386例患者中,GeneXpert MTB/RIF检测结核分枝杆菌复合群的阳性率为15.54%(60/386),MGIT960液体培养法检测结核分枝杆菌复合群的阳性率为5.44%(21/386),前者检测阳性率明显高于后者(χ²=14.52,P<0.05)。3组中,GeneXpert MTB/RIF检测阳性率分别为3.20%、24.83%和77.27%;MGIT960液体培养法检测阳性率分别为2.74%、8.28%和13.64%。GeneXpert MTB/RIF在结核性积液、脓性胸腔积液中检测结核分枝杆菌复合群的阳性率明显高于MGIT960液体培养法(χ²=14.38、17.97,P<0.05)。将采用MGIT960液体培养法检测为阳性的标本21例进行GeneXpert MTB/RIF检测发现,两种方法检测阳性结果的符合率为52.38%(11/21)。结论GeneXpert MTB/RIF检出结核分枝杆菌复合群的阳性率明显高于MGIT960液体培养法,特别是在结核性积液和脓性胸腔积液中。两种方法联合检测,有助于临床对结核性胸膜炎的早期诊断和治疗。     

泌尿生殖道解脲支原体感染的检测方法与诊断

目的 评价解脲支原体(Uu)液体培养法的诊断价值.方法 采集130例患者的泌尿生殖道分泌物、前列腺液,同时进行液体培养法和荧光定量聚合酶链反应(FQ-PCR)检测Uu,并对Uu阳性和判为污染的液体培养基进行细菌培养.然后,比较结果、分析原因.结果 130份标本中,液体培养法Uu阳性71份、判为污染的13份、阴性46份.FQ-PCR法Uu阳性66份,其中63份与液体培养法一致,另3份中,2份为液体培养法判为污染的标本、1份为液体培养法阴性标本.细菌培养结果,8份液体培养阳性而FQ-PCR法阴性的标本及13份判为污染的标本,均培养出细菌和/或真菌,菌株做脲酶和精氨酸分解试验,至少一项为阳性.结论 用液体培养法检测Uu时,阴性标本可能受分解尿素、精氨酸的细菌的影响,而误判为阳性,阳性标本可能受混浊生长的细菌影响,而误判为阴性,从而导致诊断上的错误.     

BACTEC MGIT960在二线抗结核药物药敏试验应用中的效果评价

目的 评价BACTEC MGIT 960进行4种二线抗结核药物药敏试验的效果.方法 用MGIT 960对结核分枝杆菌临床分离菌株进行二线抗结核药物卷曲霉素、卡那霉素、氧氟沙星和乙硫异烟胺的药敏检测,并将结果与L-J比例法结果进行比较分析.结果 111 株结核分枝杆菌临床分离株用MGIT960法与L-J比例法卷曲霉索、卡...     

Multicenter evaluation of the MB-Redox medium compared with radiometric BACTEC system, mycobacteria growth indicator tube (MGIT), and L?wenstein-Jensen medium for detection and recovery of acid-fast bacilli.

MB-Redox is a new manual culture system designed for the recovery of mycobacteria from clinical specimens. It consists of a liquid medium (modified Kirchner medium) containing a redox indicator, a colorless tetrazolium salt, which is reduced to colored formazan by actively growing mycobacteria. Acid fast bacilli (AFB) are easily detected in the medium as pink to purple pinhead-sized particles. We report the results of a multicenter study (involving four Italian microbiology laboratories processing 2370 clinical specimens) aiming to evaluate the recovery rates of AFB and time required for their detection by using the MB-Redox medium. Two different protocols were set up: in Protocol A (1580 specimens) the performance of MB-Redox was compared with those of the radiometric BACTEC 460 TB system (B460) and L?wenstein-Jensen medium (L-J), whereas in Protocol B (790 specimens) it was compared with those of the Mycobacteria Growth Indicator Tube (MGIT) and L-J. A total of 213 mycobacteria were recovered, including 172 Mycobacterium tuberculosis complex (MTB) isolates and 41 nontuberculous mycobacteria (NTM) isolates. In Protocol A, recovery rates were 81% for MB-Redox system, 84% for B460 system, and 77% for L-J. In Protocol B the recovery rates by individual system were 87, 83, and 76% for MB-Redox, MGIT, and L-J, respectively. Differences in both the protocols were not statistically significant. The MB-Redox system plus L-J (Combination 1) recovered 94% of the isolates in Protocol A and 93% in Protocol B, while B460 plus L-J (Combination 2) and MGIT plus L-J (Combination 3) detected 91 and 89% of all mycobacteria isolates respectively. No statistically significant differences were found among the combinations. The mean time to detection of mycobacteria was 16.3 days in Protocol A and 19.1 days in Protocol B with the MB-Redox system, 22.4 and 25.9 days with L-J, 13.2 days with B460, and 18.2 days with MGIT. The contamination rates were 2.1, 2.0, 1.9, and 3.6 for MB-Redox, B460, MGIT, and L-J respectively. The MB-Redox is a reliable, nonradiometric system for growth and detection of mycobacteria. When used in combination with a solid medium it proved to be an effective replacement for B460. The MB Redox system is a labor-intensive method requiring much handling during the visual reading procedures.     

7种实验方法诊断结核病的临床应用价值

目的评估直接涂片法、浓集涂片法、结核分枝杆菌罗氏培养法、BACTEC MGIT960系统培养结核分枝杆菌法、实时荧光定量聚合酶链反应(PCR)、实时荧光核酸恒温扩增检测技术(SAT)扩增法、结核分枝杆菌IgG抗体检测等7种方法对临床结核诊断的应用价值。方法对临床确诊的672例肺结核患者和62例健康体检者的痰标本用7种结核菌检测方法进行检测。结果 672例患者中检测阳性率分别为直接涂片法17.0%(115/676)、浓集涂片法25.6%(173/676)、改良罗氏培养法32.2%(218/676)、BACTEC MGIT960系统培养结核分枝杆菌法45.7%(309/676)、实时荧光定量PCR 90.1%(609/676)、SAT扩增法96.4%(652/676)、结核分枝杆菌IgG抗体检测法65.4%(442/676)。对照组只有荧光PCR法和结核抗体法检出阳性,阳性率分别为8.1%、12.9%。涂阴标本经罗氏培养法+结核抗体法+SAT法联合检出阳性率高于单一检测方法。结论涂片法操作简便、快速和价格便宜,但阳性率低,在长时期内其依然是基层医院诊断结核病的主要检测手段;结核分支杆菌培养能进行菌种鉴定和药物敏感试验仍是不可缺少的方法,但周期长;结核抗体法方便,简单,仍是结核实验室诊断的重要手段,但不能区分感染与发病;荧光PCR扩增法快速,灵敏,有较好诊断价值,但设备昂贵,技术要求高,易污染;SAT法快速,灵敏,要求较荧光PCR扩增法低,可以辅助诊断结核病。同时联合检测提高诊断率,并满足不同临床需要。     

Evaluation of the BACTEC MGIT 960 in comparison with BACTEC 460 TB for detection and recovery of mycobacteria from clinical specimens

The BACTEC MGIT system (M960), a fully automated, non radiometric instrument, designed for rapid detection of acid fast bacilli (AFB) from clinical specimens, was compared with the radiometric BACTEC 460 system (B460) and L?wenstein-Jensen (L-J) solid medium. A total of 1,093 respiratory and extrapulmonary specimens were decontaminated by the NALC-NaOH standard method, and randomly inoculated into the media. A total of 122 mycobacteria were recovered, including 47 Mycobacterium tuberculosis complex (MTB) isolates and 75 nontuberculous mycobacteria (NTM) isolates. Overall recovery rates were 59% for M960 system (p < 0.0001), 58.2% for L-J. medium (p < 0.0001) and 82% for Bactec 460 system, whereas rates for MTB alone were 91.5, 76.6 (p = 0.007), and 95.7%, respectively. The combination of M960 or B460 systems with L-J medium showed the same recovery rates for MTB strains (97.9%), whereas NTM rates were 68% (p < 0.0001) and 93.3%, respectively. Mean time to detection of smear-positive MTB, smear-negative MTB, and NTM were 12.2, 13.4, and 23.3 days, respectively, with the M960, 11.7, 21.3, and 24.8 days with the B460 and 20.4, 28.7, and 28.4 days with L-J medium. The M960 system showed a contamination rate of 9.8%, while B460 and L-J medium showed contamination rates of 4.3 and 3.8% respectively. In conclusion, the M960 system appeared to be accurate and rapid for the recovery of MTB, but reduced recovery of NTM and a high number of contaminated cultures deserve further study in order to assess if this system can represent a valuable alternative to the radiometric system.     

变色液体培养基快速检测分枝杆菌

目的评价变色液体培养基快速检测分枝杆菌的应用价值.方法利用变色液体培养基接种痰标本培养,并与改良酸性罗-琼氏(Lowenstein-Jensen,L-J)培养基平行比较.结果 911例痰标本的涂阳检测率为29.1%,变色液体培养阳性率为43.4%,略高于改良罗-琼氏(L-J)培养阳性率40.1%,变色液体培养基和改良罗-琼培养涂阳平均检出时间分别为6和18 d,涂阴平均检出时间分别为15和28 d,污染率分别为2.7%和2.1%.结论变色液体培养基检测结核分枝杆菌,具有快速、简便、可靠和实用性好的特点.     

Evaluation of the BACTEC MGIT 960 system for the recovery of mycobacteria

We evaluated the BACTEC MGIT 960 system, which is a fully automated, non-invasive, continuous monitoring system for the growth and detection of mycobacteria. Including respiratory and other specimens, 1,742 specimens were processed and inoculated into the BACTEC MGIT 960 and the BACTEC 460 TB Systems, as well as onto Lowenstein-Jensen (L-J) media. A total of 104 isolates of mycobacteria were recovered from all culture systems. This included Mycobacterium tuberculosis complex, Mycobacterium avium-intracellulare (MAI) complex and other mycobacteria (MOTT). The isolation rates for M. tuberculosis complex and MAI complex were comparable for the BACTEC 460 (54.8% and 13.5%) and the BACTEC MGIT 960 (51.9% and 13.5%). The overall isolation rate was less for BACTEC MGIT 960 (76.9%) which was due to lesser number of MOTT isolates recovered from this system. The mean times to detection (TTD) for all mycobacteria were 9.3 days for the BACTEC MGIT, 14.6 days for the BACTEC 460 and 21.6 days for L-J. A significant difference was observed when TTD was tested in relation to degree of positivity in smears, with the BACTEC MGIT maintaining the short TTD even with less number of bacilli in the smear. The contamination rates were, 6.4% for BACTEC MGIT, 2.9% for BACTEC 460 and 12.1% for L-J medium. The BACTEC MGIT 960 system shows performances comparable to the BACTEC 460 and seems to be a dependable, user friendly system.     

Evaluation of the BDProbeTec ET system as screening tool in the direct detection of mycobacterium tuberculosis complex in respiratory specimens

We evaluated the BDProbeTec ET System (Becton Dickinson) for the routine detection of Mycobacterium tuberculosis complex (MTC) in respiratory specimens and pleural fluids, comparing with microscopy (Ziehl Neelsen stain, ZN) and culture in liquid (BACTEC MGIT 960, MGIT) and solid (L?wenstein Jensen, LJ) media. Five hundred and two specimens, collected from 266 patients, of which 257 with suspected tuberculosis and 9 receiving anti-tuberculosis treatment, were investigated. Thirty-nine specimens were positive by any method, including false positives. Mycobacteria were isolated from 33 specimens (32 Mycobacterium tuberculosis and 1 Mycobacterium chelonae). Thirty-six specimens were BDProbeTec ET positive, 33 specimens were MGIT positive, 27 were LJ positive and 22 were ZN positive. With BDProbeTec ET, 2 specimens were false negative (culture positive), and 2 specimens from non-treated patients were false positive (culture negative). The overall sensitivity, specificity, and positive and negative predictive values for BDProbeTec ET compared to culture were 93.7, 98.7, 83.3, and 99.5%, respectively, while with smear-positive and smear-negative specimens the sensitivities were 100% and 81.5% respectively. In five treated patients the disappearance of MTC could be monitored using BDProbeTec ET in parallel with culture. The overall inhibition rate was 0.2%. BDProbeTec ET can be very useful for rapid detection of MTC, especially in smear-negative respiratory specimens.     

Comparison of Lowenstein-Jensen medium and MGIT culture system for recovery of Mycobacterium tuberculosis from abscess samples

PurposeIn this study, our aim was to assess Lowenstein-Jensen (L-J) medium and MGIT culture system for recovery of Mycobacterium tuberculosis (MTB) from abscess samples in skeletal tuberculosis (TB) cases.MethodsAbscess samples were collected from patients suggestive of skeletal TB in Beijing Chest Hospital for laboratory examination, including smear microscopy, L-J culture and MGIT culture.ResultsOf the 232 abscess samples, 72 (31.0%) were culture-positive for mycobacteria. Of 72 isolates recovered, 94.4% were detected in MGIT 960 and 75.0% on L-J medium. MGIT could recover significantly higher rate of MTB isolates from smear-positive specimens than L-J medium. The mean time to detection of MTB in MGIT 960 was significantly lower than that on L-J medium.ConclusionThe BACTEC MGIT 960 outperforms the conventional L-J medium in recovering MTB from abscess samples. The combination of MGIT and L-J method also increases the overall recovery rate of MTB in culture.