脑出血大鼠脑内移植神经干细胞后再生相关因子的表达

目的观察大鼠脑出血模型脑内移植神经干细胞(NSCs)后脑源性神经营养因子(BDNF)和血管内皮生长因子(VEGF)的表达及对血管生成的影响。方法 SD大鼠140只,随机分为假手术组(A组)、脑出血模型组(B组)、NSCs培养液移植组(C组),NSCs移植组(D组),每组35只。免疫组织化学方法观察各组术后6 h、1、4、7、14、21和28天BDNF、VEGF及CD34标记的微血管密度的变化,并进行相关分析。结果与A组比较,B组、C组BDNF的表达1天达高峰(P<0.05),D组表达高峰延迟至第4天,至28天仍高于A组(P<0.05);D组在所有时间点与B组比较,差异有统计学意义(P<0.05);D组在4天后的5个时间点与C组比较,差异有统计学意义(P<0.05)。B组、C组VEGF和微血管密度表达模式相同,均在1天达高峰;D组呈双高峰表达,在第4天和14天,至28天仍高于B组、C组的表达水平(P<0.05);相关分析显示,VEGF与微血管密度呈正相关(r=0.911,P<0.05)。结论脑出血大鼠脑内移植NSCs后,脑内BDNF及VEGF维持长时间的高表达,微血管密度增加。     

马来丝虫肌球蛋白29表位基因真核表达质粒及原核表达蛋白免疫小鼠诱导的免疫应答

目的观察马来丝虫肌球蛋白29(Brugia malayi myosin 29,Bm M29)表位基因真核表达质粒pc DNA3.1(+)-Bm M29和原核表达的纯化重组蛋白r Bm M29免疫小鼠诱导的免疫应答效果。方法在大肠埃希菌(Escherichia coli)BL21诱导表达重组蛋白r Bm M29,纯化后作为重组蛋白疫苗;纯化真核表达重组质粒pc DNA3.1(+)-Bm M29作为核酸疫苗。核酸疫苗免疫注射部位为小鼠后腿胫前肌,重组蛋白为皮下多点注射。60只BALB/c小鼠随机分为A、B、C、D、E组,每组12只,分别注射PBS(100μg)、pc DNA3.1(+)/Cp G(100μg/30μg)、pc DNA3.1(+)-Bm M29/Cp G(100μg/30μg)、r Bm M29/Cp G(50μg/30μg)、pc DNA3.1(+)-Bm M29/r Bm M29/Cp G(前2次注射pc DNA3.1(+)-Bm M29/Cp G 100μg/30μg,第3次注射r Bm M29/Cp G 50μg/30μg),共免疫3次,每次免疫间隔2周。初次免疫后第4、6、8周,眼球摘除法采血制备血清,ELISA法检测免疫小鼠血清特异性Ig G抗体效价。免疫第8周处死小鼠,制备脾细胞悬液培养48 h,ELISA检测培养上清中细胞因子γ干扰素(IFN-γ)、白细胞介素-4(IL-4)水平。结果 ELISA检测结果显示,A、B、C、D和E组小鼠初次免疫后第4周血清中抗体的吸光度(A490值)分别为0.038±0.050、0.053±0.009、0.360±0.035、0.456±0.025、0.370±0.025,第6周分别为0.045±0.003、0.045±0.005、0.510±0.018、0.548±0.010、0.552±0.018,第8周分别为0.041±0.004、0.044±0.009、0.606±0.047、0.674±0.042、0.770±0.041,C、D、E组均高于A、B组(P0.05),第8周时,E组Ig G抗体的A490值高于C组和D组(P0.05)。初次免疫后第8周,A至E组小鼠脾细胞培养上清中IFN-γ的水平分别为(47.72±8.94)、(50.43±2.81)、(304.78±8.42)、(242.28±5.99)、(426.52±6.76)pg/ml,C、D、E组均高于A、B组(P0.05),E组高于C组和D组(P0.05);小鼠脾细胞培养上清中IL-4的水平分别为(60.00±11.14)、(57.71±15.95)、(93.17±12.56)、(96.67±11.48)、(101.17±5.81)pg/ml,C、D、E组均高于A、B组(P0.05)。结论 pc DNA3.1(+)-Bm M29核酸疫苗和r Bm M29重组蛋白均可诱导BALB/c小鼠产生特异性体液和细胞免疫应答,核酸疫苗与重组蛋白疫苗联合免疫效果更佳。     

结核分枝杆菌Ag85A／ESAT-6嵌合基因DNA疫苗与抗痨药物联合治疗小鼠结核病

目的 研究结核分枝杆菌Ag85A/ESAT-6嵌合基因DNA疫苗及其与抗痨药物联合治疗小鼠结核病的效果.方法 用结核分枝杆菌临床分离的利福平和异烟肼敏感株HB240-1尾静脉注射17～19g的6～8周龄雌性BALB/c小鼠后,将小鼠随机均匀地分为5组,感染后第3d开始,分别用pVAX1空载体(A组)、利福平和异烟肼(B组)、利福平、异烟肼和Ag85A/ESAT-6嵌合基因DNA疫苗(C组)、Ag85A/ESAT-6嵌合基因DNA疫苗(D组)、HSP65基因DNA疫苗(E组)治疗40d,每组10只小鼠.治疗结束后2w,分别取肺、肝和脾观察病理改变、称取重量、做菌落计数.结果 小鼠感染5w后,肺内菌量可达到1.8×106 CFU,脾内菌量达到1.7×106CFU.治疗结束后2w,B组和C组肺、脾脏器重量指数显著低于D组和E组,D组和E组脾脏器重量指数低于A组,但差别不显著.A组肺脏病变程度最重,病变范围80%～100%,干酪样坏死灶为弥漫性;D组和E组肺脏病变范围50%,干酪样坏死灶为片状;B组和C组肺脏病变程度较轻,病变范围较小,干酪样坏死灶为点状.A组脾脏9只重度肿大;B组和C组脾脏未见明显病变;D组和E组脾脏各有4只重度肿大.与A组相比,E组、D组、B组和C组肺脏菌落数分别减少了45%、50%、98.9%、99%;脾脏菌落数依次减少了50%、55%、99.2%、98.4%.与pVAX1空载体治疗组相比,各治疗组均有不同程度的疗效,Ag85A/ESAT-6嵌合基因疫苗组与HSP65基因疫苗组疗效相当.结论 Ag85A/ESAT-6嵌合基因DNA疫苗组联合化疗的疗效明显强于Ag85A/ESAT6嵌合基因DNA疫苗单独应用.     

灵芝孢子油对辐射损伤老龄小鼠的保护作用

目的探讨灵芝孢子油对辐射损伤老龄小鼠的保护作用。方法将144只健康老龄(12月龄)SPF级昆明雄性小鼠随机分为A、B、C三组,各组随机分为辐射模型组、低、中、高剂量灵芝孢子油组,每组12只。低、中、高剂量灵芝孢子油组小鼠经口灌胃浓度分别为12.5、25.0和75.0 g/L的灵芝孢子油,辐射模型组灌胃等体积的玉米油,灌胃容量为0.02 ml/g,1次/d。灌胃第30天,A组小鼠检测外周血白细胞计数后,各组小鼠给予60 Coγ射线全身一次性照射,照射后继续给予受试物。A组小鼠分别在照射后第3、14天检测外周血白细胞计数;B组照射后第4天检测股骨及胸骨骨髓细胞DNA含量及骨髓细胞微核率;C组小鼠照射后第7天测定红细胞及肝脏超氧化物歧化酶(SOD)活力。结果灵芝孢子油暴露后,与辐射模型组相比,中、高剂量灵芝孢子油组小鼠外周血白细胞计数、红细胞及肝脏SOD活力显著升高(P<0.01),高剂量组骨髓细胞微核率显著降低(P<0.05);低、中、高剂量组小鼠骨髓细胞DNA含量均明显增多(P<0.05)。随着灵芝孢子油灌胃剂量的升高,小鼠骨髓细胞微核率呈下降趋势,而外周血白细胞计数、骨髓细胞DNA含量、红细胞及肝脏SOD活力呈上升趋势。结论灵芝孢子油对辐射引起的老龄小鼠损伤具有拮抗作用。     

结核分支杆菌MPT64质粒DNA的免疫原性和保护效力

目的　研究结核分支杆菌 (Mycobacterium tuberculosis,MTB)MPT64质粒DNA的免疫原性和保护效力。方法 分别用生理盐水 (A)、载体质粒 (B)、卡介苗 (C)和MPT64重组质粒 (D)免疫小鼠,通过ELISA方法检测小鼠血清中抗MPT64抗体,免疫 8周后以MTB腹腔内攻击小鼠,攻击 4或 9周后,分别取肺、肝和脾脏观察病理改变、称取重量、作菌落计数及脾淋巴细胞转化试验。结果 D组1 0只小鼠中只有 4只血清中检测出抗MPT64抗体。MTB攻击 4周后,D组鼠脾淋巴细胞转化率显著高于其它 3组,A、B、D组肺、肝和脾重量明显高于C组,A组脾菌落数较其它 3组明显增多,其余脏器菌落数各组无明显区别,A、B组可见肺部严重病变,C组未见明显病变,D组存在个体差异。MTB攻击 9周后,A、B组鼠脾淋巴细胞转化率较C组显著降低,D组显著增高,各组肺、肝和脾重量无显著差别,C组肝菌落数明显低于其它 3组,其余脏器各组间无显著差别,各组鼠肺表面均可见不同程度的病变。所有鼠肝、脾均未见明显病变。结论 MTBMPT64DNA疫苗经肌肉注射免疫小鼠能诱发特异的体液和细胞免疫应答,对小鼠抗MTB感染具有一定的免疫保护效力,但比卡介苗差。     

热休克法制备的DC肿瘤疫苗治疗小鼠乳腺癌效果观察

目的观察热休克法制备的树突状细胞（DC）肿瘤疫苗治疗小鼠乳腺癌的疗效。方法将80只6～8周龄BALB/c乳腺癌小鼠模型随机分为4组各20只,A组皮下注射热休克后冻融法制备的DC肿瘤疫苗,B组注射单纯冻融法制备的DC肿瘤疫苗,C组注射生理盐水,D组不做任何处理,干预后21 d用卡尺测量肿瘤的纵径（a）和横径（b）,计算肿瘤体积（1/6πab^2）;21 d取血后脱颈处死小鼠,完整剥离肿瘤,称重。采用ELISA法检测各组IL-2、IL-12I、FN-γ水平。结果 A组肿瘤体积和肿瘤重量明显低于B组、C组,B组明显低于C组,P均〈0.05;A组、B组、C组IL-2I、L-12I、FN-γ水平均明显高于D组,A组、B组明显高于C组,A组明显高于B组,P均〈0.05。结论热休克法制备的DC肿瘤疫苗较冻融法制备的DC肿瘤疫苗能在小鼠体内激发更强的免疫反应,对小鼠乳腺癌有更好的治疗效果。     

抗心律失常药对截肢大鼠炎性因子表达及心肌电活动的影响

目的:观察抗心律失常药对截肢大鼠炎性因子表达及心肌电活动的影响。方法:选取30只健康清洁级雄性SD大鼠,随机分为A组、B组、C组、D组和E组,每组6只,建立左后肢截肢手术创伤模型,其中A组给予生理盐水,B组、C组、D组和E组分别给予利多卡因(2 mg/kg)、普萘洛尔(7 mg/kg)、胺碘酮(200 mg/kg)和维拉帕米(20 mg/kg),记录并比较手术前后大鼠心率、QT间期、PR间期、单相动作电位(MAP)振幅、左室收缩压(LVSP)变化,及肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)和C反应蛋白(CRP)水平。结果:C组、D组和E组术后15 min、30 min、60 min时心率明显低于A组和B组(P<0.05);B组、C组、D组和E组术后15 min、30 min、60 min和120 min时PR间期、QT间期高于A组(P<0.05);B组术后15 min、30 min、60 min和120 min时MAP振幅明显低于A组、C组、D组和E组(P<0.05);B组、C组、D组和E组术后15 min、30 min、60 min和120 min时LVSP明显低于A组(P<0.05);A组、B组、C组、D组和E组术后2 h时血清TNF-α、IL-6和CRP比较差异均无统计学意义。结论:抗心律失常药有助于减轻截肢创伤对大鼠心肌电活动的影响,但对炎性因子水平无明显作用。     

不同频率肺复张术在单肺通气患者中应用效果的对比研究

目的比较不同频率肺复张术(RM)在单肺通气(OLV)患者中的应用效果。方法选取2014年8月—2015年8月鄂东医疗集团市中医医院收治的拟行开胸手术及OLV的患者88例,根据RM频率分为A、B、C、D组,每组22例。OLV期间,A、B、C组患者RM频率分别为30 min/次、60 min/次、120 min/次,D组患者仅在关胸前行1次RM。比较4组患者手术相关指标(包括OLV时间、手术时间、补液量、失血量),OLV开始时(T1)、OLV30 min(T2)、OLV 1 h(T3)、OLV 2 h(T4)、OLV结束时(T5)、恢复双肺通气30 min(T6)氧合指数(OI)和肺内分流量(Qsp),T1、T3、T6、术后2 h(T7)、术后24 h(T8)血清肿瘤坏死因子α(TNF-α)和白介素6(IL-6)水平。结果 (1)4组患者OLV时间、补液量、失血量比较,差异无统计学意义(P>0.05);B组患者手术时间短于A、C、D组(P<0.05)。(2)时间与方法在OI和Qsp上存在交互作用(P<0.05);时间在OI和Qsp上主效应显著(P<0.05);方法在OI和Qsp上主效应显著(P<0.05)。T2时,B、C、D组患者OI低于A组(P<0.05);T3时,B组患者OI高于A、C、D组,C、D组患者OI低于A组(P<0.05);T4时,B组患者OI高于A、C、D组,C、D组患者OI低于A组,D组患者OI低于C组(P<0.05);T5、T6时,B组患者OI高于A、C、D组,C组患者OI高于A、D组,D组患者OI低于A组(P<0.05)。T2时,B、C、D组患者Qsp高于A组(P<0.05);T3时,B、C、D组患者Qsp高于A组,B组患者Qsp低于C、D组(P<0.05);T4、T5时,B组患者Qsp低于A、C、D组,C、D组患者Qsp高于A组,D组患者Qsp高于C组(P<0.05);T6时,B组患者Qsp低于A、C、D组,C组患者Qsp低于A、D组,D组患者Qsp高于A组(P<0.05)。(3)时间与方法在血清TNF-α和IL-6水平上存在交互作用(P<0.05);时间在血清TNF-α和IL-6水平上主效应显著(P<0.05);方法在血清TNF-α和IL-6水平上主效应显著(P<0.05)。T3时,B、C、D组患者血清TNF-α水平低于A组,B组患者血清TNF-α水平高于C、D组(P<0.05);T6、T7、T8时,B、C、D组患者血清TNF-α水平低于A组,B组患者血清TNF-α水平低于C、D组,D组患者血清TNF-α水平高于C组(P<0.05)。T3时,B、C、D组患者血清IL-6水平低于A组,B组患者IL-6水平高于C、D组(P<0.05);T6、T7、T8时,B、C组患者血清IL-6水平低于A组,B组患者IL-6水平低于C、D组,D组患者血清IL-6水平高于C组(P<0.05)。结论 OLV期间每60 min行1次RM可有效改善OLV患者肺氧合功能,降低Qsp并减轻炎性反应,应用效果较佳。     

结核分支杆菌MPT64质粒DNA的免疫原性和保护效力

目的：研究结核分支杆菌（Mycobacterium tuberculosis,MTB)MPT64质粒DNA的免疫原性和保护效力。方法：分别用生理盐水（A）、载体质粒（B）、卡介苗（C）和MPT64重组质粒（D）免疫小鼠，通过ELISA方法检测小鼠血清中抗MPT64抗体，免疫8周后以MTB腹腔内攻击小鼠，攻击4或9周后，分别取肺、肝和脾脏观察病理改变、称取重量、作菌落计数及脾淋巴细胞转化试验。结果：D组10只小鼠中只有4只血清中检测出抗MPT64抗体。MTB攻击4周后，D组鼠脾淋巴细胞转化率显著高于其它3组，A、B、D组肺、肝和脾重量明显高于C组，A组脾菌落数较其它3组明显增多，其余脏器菌落数各组无明显区别，A、B组可见肺部严重病变，C组未见明显病变，D组存在个体差异。MTB攻击9周后，A、B组鼠脾淋巴细胞转化率较C组显著降低，D组显著增高，各组肺、肝和脾重量无显著差别，C组肝菌落数明显低于其它3组，其余脏器各组间无显著差别，各组鼠肺表面均可见不同程度的病变。所有鼠肝、脾均未见明显病变。结论：MTB MPT64 DNA疫苗经肌肉注射免疫小鼠能诱发特异的体液和细胞免疫应答，对小鼠抗MTB感染具有一定的免疫保护效力，但比卡介苗差。     

生晒参超微饮片对脾虚小鼠小肠推动及其机制研究

[目的]探讨生晒参超微饮片对脾虚小鼠小肠推动的影响及其作用机制。[方法]选用雄性昆明种小鼠,随机分为正常（A）组、模型（B）组、生晒参传统饮片（C）组、生晒参超微饮片全剂量（D）组及生晒参超微饮片1／2剂量（E）组,采用利血平法制备脾虚动物模型,分别给予0．85％氯化钠、生晒参传统饮片及生晒参超微饮片,观察各组体重变化及对脾虚小鼠小肠推动率的影响,运用RT-PCR方法观察用药后各组钙调蛋白（CAM）mRNA表达水平。[结果]造模小鼠均出现小肠推动率加快,各治疗组均不同程度改善脾虚小鼠小肠运动功能。其中C、D、E组与B组比较差异均有统计学意义（P〈0．05）;C、D、E组之间差异均无统计学意义。A组小鼠小肠有一定水平的CAM表达,造模后CAMmRNA表达增高;C、D、E组CAMmRNA表达下降,其中C、D组与B组比较以及C、D组间比较差异均有统计学意义（P〈0．05）。[结论]生晒参超微饮片通过影响CAMmRNA表达水平,调节脾虚小鼠小肠运动,对小肠功能紊乱有调节作用;且对CAMmRNA表达水平的调节作用优于生晒参传统饮片。     

三七总皂苷对老年大鼠学习能力、记忆力及海马神经元凋亡的影响研究

目的探讨三七总皂苷(PNS)对老年大鼠学习能力、记忆力及海马神经元凋亡的影响。方法选择22月龄雌性Sprague-Dawley(SD)大鼠45只,随机分为低剂量组15只、高剂量组15只、老年对照组15只;另选择2月龄雌性SD大鼠15只作为青年对照组。低剂量组大鼠每天注射1次PNS,剂量为50μg/kg;高剂量组大鼠每天注射1次PNS,剂量为100μg/kg;青年对照组和老年对照组大鼠均注射等量0.9%氯化钠溶液。各组大鼠均连续注射30 d。对各组大鼠进行学习能力、记忆力测试,计算其正确反应率;采用TUNEL法检测海马CA1区神经元凋亡情况。结果青年对照组、低剂量组、高剂量组学习能力及记忆力测试正确反应率高于老年对照组,高剂量组学习能力及记忆力测试正确反应率高于低剂量组(P0.05)。青年对照组、低剂量组、高剂量组海马CA1区TUNEL阳性细胞数低于老年对照组,高剂量组海马CA1区TUNEL阳性细胞数低于低剂量组(P0.05)。结论 PNS能改善老年大鼠的学习能力和记忆力,减少海马神经元凋亡,大剂量应用时效果较好。     

蛋白酶抑制剂对全氟异丁烯吸入致 ALI 大鼠氧化应激的影响

目的：研究探讨蛋白酶抑制剂(抑肽酶)对全氟异丁烯(PFIB)吸入致 ALI 大鼠炎症因子的影响。方法健康清洁级成年雄性 Sprague-Dawley 大鼠40只,根据体质量随机分成5组：生理盐水对照组(简称 A 组)、ALI 组(简称 B 组)、抑肽酶治疗组(5 mg/kg、15 mg/kg 和30 mg/kg,简称 C、D、E 组,),每组8只。将 B、C、D、E 组大鼠经头部暴露于自制 PFIB 动态吸入染毒系统装置染毒5 min。染毒30 min 后,各组大鼠均经腹腔注射生理盐水或配置好的不同浓度抑肽酶溶液。24 h后结束实验,处死大鼠,获取 BALF、肺组织样本,检测肺湿重与肺干重比(W/D),BALF 总蛋白含量,肺组织匀浆中超氧化物歧化酶(SOD)、丙二醛(MDA)的含量,光镜下肺组织病理学和免疫组织化学检查,并将数据进行统计学分析。结果 B 组、C 组、D 组、E 组的肺湿与干重比、BALF总蛋白含量和 MDA 含量升高,与 A 组比较差异有统计学意义(P <0.05),B 组、C 组、D 组、E 组的 SOD 含量下降,与 A 组比较差异有统计学意义(P <0.05)。E 组肺湿与干重比、BALF 总蛋白及MDA 含量均低于 B 组、C 组、D 组,差异有统计学意义(P <0.05);E 组 SOD 含量高于 B 组、C组、D 组,差异有统计学意义(P <0.05)。肺组织病理学检查显示,C 组、D 组、E 组较 B 组的肺组织水肿情况减轻、炎性细胞浸润减少,肺损伤程度降低,以高剂量最为显著。免疫组织化学检查示, C 组、D 组、E 组的核因子κB 的表达较 B 组为低。结论蛋白酶抑制剂抑肽酶能通过抗氧化应激及抑制炎性反应,来减轻 PFIB 吸入导致的 ALI。     

Correlation of serum IgE levels with severity of acute pulmonary thromboembolism

We aimed to investigate whether there is a direct correlation of serum IgE concentration with severity of acute pulmonary thromboembolism (PTE). DESIGN: Prospective study. SETTINGS: University medical center. Forty-six patients (27 female, 19 male) who were diagnosed as acute PTE in our clinic between 01 October 2000 and 30 November 2001 comprised the study group. Mean age was 55 (range was 20-82). The study group was divided into three groups according to severity of PTE: Group A, submassive PTE without pulmonary infarction (20 patients); group B, submassive PTE with pulmonary infarction (15 patients); and group C, massive PTE (11 patients). Serum IgE concentrations were measured by ELISA method at 1st, 5th, 15th, 30th, 60th, 90th days, and 120th days, if needed, after the diagnosis. Statistical analysis was made by Post hoc Tukey test. First day serum IgE levels were highest in group B (mean 507.7) followed by group C (mean 324.2), and were lowest in group A (mean 117.2). The differences between group B and group C, between group B and group A, and also between group C and A were all statistically significant (p< 0.5, p< 0.0001, p< 0.015, respectively). 5th day and 15th day results showed statistically significant differences between group B and A, and between group C and A (at 5th day: p<0.0001, p< 0.015 respectively, and at 15th day: p< 0.0001, p< 0.012 respectively). At 30th, 60th, and 90th days of diagnosis serum IgE concentrations were higher in group B than in group A which were statistically significant (p< 0.0001, p< 0.0001, p< 0.019 respectively). Patients with submassive PTE and pulmonary infarction had the highest serum IgE concentrations and the longest duration of high levels of IgE.     

抗纤维化治疗对大鼠支气管肺泡灌洗液中头孢噻肟浓度的影响

目的观察抗肺纤维化药物对支气管肺泡灌洗液（BALF）中头孢噻肟浓度的影响。方法将90只Wister雄性大鼠随机分成五组,即正常对照组（A组）、模型组（B组）、干扰素组（C组）、盐酸氨溴索组（D组）、地塞米松组（E组）各18只,除A组外均采用气管内注入博来霉素的方法制作肺间质纤维化模型。造模后C、D、E组分别每天腹腔内注入γ-干扰素3万IU/只、盐酸氨溴索7 mg/只、地塞米松0.1 mg/只。各组分别于给药后第7、14、28天,每次取6只大鼠,鼠尾静脉注射头孢噻肟600mg/kg后行支气管肺泡灌洗,取BALF采用液相色谱-质谱联用法测定头孢噻肟浓度,并取肺组织做病理观察。结果第7天D组、E组BALF头孢噻肟浓度低于B组,C组高于B组,E组与B组有显著性差异（P=0.012）。第14天D组、E组BALF头孢噻肟浓度升高,而C组下降,三组均高于B组,D组与B组有显著性差异（P=0.000）。第28天E组头孢噻肟浓度继续升高,明显高于其他各组,而D组锐降,C组继续下降,且均低于B组,C、E组与B组有显著性差异。结论地塞米松可提高纤维化期BALF中头孢噻肟的浓度。     

Effect of ethanol consumption on colon cancer in an experimental model.

AIMS: The present study was designed to examine the effect of an ethanol supplement on experimental colon carcinogenesis. MATERIAL AND METHODS: One hundred and ten 10-week-old Sprague-Dawley rats were divided into five groups: group A (20 rats) received no treatment. Group B (20 rats) received a supplement of ethanol at 1.23 g/kg of body weight per day added to their drinking water for 24 weeks. Group C (30 rats) received 18 weekly doses of dimethylhydrazine (DMH) at 21 mg/kg of body weight from the beginning of the study. Group D (20 rats) received ethylen-diamin-tetracetic acid (EDTA) solution only for 18 weeks. Group E (20 rats) received ethanol at the same dose as group B plus DMH injections at the same dose as the rats in group C from the beginning of the study. All experimental animals were sacrificed after 25-27 weeks. RESULTS: No significant differences in the number of rats that developed tumors, number of tumor-free animals, and number of tumors per rat, as well as in macro-microscopic tumoral findings were observed for animals in group C compared to animals in group E. CONCLUSIONS: We concluded that the addition of an ethanol supplement does not modify colorectal carcinogenesis using a dynamic model of tumor induction with DMH.     

Iodine absorption after intraoperative bowel irrigation with povidone-iodine

PURPOSE: Povidone-iodine is a commonly used intrarectal tumoricidal agent in patients undergoing colorectal surgery. The aim of this study was to assess systemic absorption of total iodine and its effect on thyroid function after intrarectal application. METHODS: Twenty patients with carcinoma of the rectum received intraoperative irrigation with either povidone-iodine (Group A; n=10) or physiologic saline (Group B; n=10). Ten patients with carcinoma of the sigmoid colon (group C) were treated the same as Group A. Electrolyte, total iodine, triiodothyronine, thyroxine, and thyroid-stimulating hormone values were measured in serum preoperatively and before intraoperative irrigation and immediately, ten minutes, 1 hour, 6 hours, 24 hours, and two weeks after irrigation. RESULTS: No significant changes occurred in serum electrolytes. A significant uptake of the total iodine was demonstrated in each group. Total iodine levels examined immediately, ten minutes, and one hour after irrigation in Group C were significantly higher than those examined in Group B. Maximum values were obtained one hour after irrigation in Groups A and B and six hours after irrigation in Group C. No significant changes occurred in triiodothyronine, thyroxine, and thyroid-stimulating hormone levels among the three groups. The decrease in triiodothyronine levels after surgery was demonstrated in each group. We noted a decrease after surgery in thyroxine levels for Groups A and B and in thyroid-stimulating hormone levels for Group B. Those hormones were not affected by the administration of povidone-iodine. CONCLUSION: High serum levels of iodine did not cause organ toxicity, suggesting that a single use of intraoperative bowel irrigation with povidone-iodine may be performed with practically negligible risk.Supported in part by SRL, Inc., Tokyo, Japan.Poster presentation at the American Society of Colon and Rectal Surgeons' 100th Anniversary and Tripartite Meeting, Washington, D.C., May 1 to 6, 1999.     

The effects of high iodine on the brain development of mice

C57 mice were divided into two groups: (A): experiment group (H group). Each female mouse was injected with 20 microliter iodized oil; (B): control group (N group). Both groups were mated 8 days after iodized oil injection. Urine iodine contents/24 h of H group was 20 times higher than that in N group 7 days after injection and 18 times higher two months later. Serum T4 values were determined. Brain protein, DNA, body weight, motility and learning ability were measured in the progeny. The results indicate that there was no statistically significant difference between the groups in any of the parameters mentioned above. This strongly suggests that a high iodine dose, given as iodized oil, does not have obvious damaging effects on brain development, at least in mice.     

Experiment research of cisplatin implants inhibiting transplantation tumor growth and regulating the expression of KLK7 and E-cad of tumor-bearing mice with gastric cancer

Objective: To study the influence of cisplatin implants on transplantation tumor growth and the expression of tissue kallikrein-7(KLK7) and E-cadherin(E-cad) in tumor-bearing mice with gastric cancer. Methods: BALB/c nude mice were collected as experimental animal and were randomly divided into model control group(Group A), tail intravenous injection of cisplatin group(Group B), intratumor injection of cisplatin group(Group C) and cisplatin implants treatment group(Group D). After the drugs intervening, the weight and volume of transplantation tumors were measured on Day 20, Day 30 and Day 40 and serum and KLK7 and E-cad contents in transplanted tumor tissue were examined. Results: On Day 20, Day 30 and Day 40 after treatment, the weight and volume of transplantation tumors of tumorbearing mice in four groups were different(Group A Group B Group C Group D). The contents of KLK-7 and E-cad in tumor tissue and serum of tumor-bearing mice in four groups were different(Group A Group B Group C Group D in KLK-7) and(Group A Group B Group C Group D in E-cad). The weight and volume, and KLK7 and E-cad contents of transplantation tumors in four groups were significant difference(P0.05). Conclusion: Cisplatin implants can inhibit the growth of transplanted tumor tissue and down-regulated KLK7 expression and up-regulated E-cad expression of tumor-bearing mice with gastric cancer     

光动力疗法根除小鼠胃幽门螺杆菌疗效观察

目的探讨不同光敏剂及不同光照射强度下光动力疗法（PDT）对小鼠胃幽门螺杆菌（Hp）的根除疗效。方法 75只雄性C57BL/6小鼠禁食12 h后,将Hp SS1制备的悬液连续灌胃3次,6周后随机分为5组（每组15只）：2个血卟啉衍生物（HpD）灌胃组（A1、A2组）;2个亚甲蓝（MB）灌胃组（B1、B2组）;1个感染对照组（C组）;另取30只小鼠分为2个为非感染对照组（D、E组）。C、D、E组给生理盐水灌胃。采用PDT照射胃体,A1、A2、D组照射波长630 nm,B1、B2、E组为532 nm,功率分别为50、100、100 mW/cm2,能量密度累积15 J/cm2;4周后取胃窦和胃体部组织行快速尿素酶试验和改良吉姆萨染色及HE染色。结果结合快速尿素酶试验和改良吉姆萨染色结果显示A2组Hp阴性率为73.33%,高于其他各组（P均〈0.05）;HE染色显示各PDT治疗组胃黏膜炎症均明显减轻。结论在合适光敏剂和光照强度下,PDT可杀灭Hp,减轻Hp相关性胃炎,不损伤正常胃黏膜。