交联透明质素特性的研究

目的研究交联透明质素的特征和性能,为其应用于临床提供理论依据。方法采用扫描电镜-能谱仪分析交联透明质素的化学成分。采用傅立叶变换红外光谱法对交联透明质素结构进行分析。使用旋转流变仪测定交联透明质素凝胶的储能模量、耗能模量及粘度等流变学性能的动态变化。采用激光粒度仪测定凝胶中微粒的大小及分布。结果能谱分析显示交联透明质素中主要成分与天然透明质素相同,但多了硫原子,该元素来自交联剂。红外光谱测定显示,交联透明质素的吸收特征基本与未交联透明质素相似,只是在示硫砜键振动的吸收加强。交联透明质素凝胶的储能模量、耗能模量显著高于天然透明质素凝胶,与频率的依赖性不明显,表现为强凝胶弹性体特征,凝胶微粒平均粒径约为500μm。结论交联透明质素是天然透明质素与二乙烯基砜交联反应形成的多聚物,该多聚物溶胀于水后生成水不溶性凝胶。交联透明质素微粒凝胶制剂具有稳定性好和粘弹性高等优点,有利于进一步拓展其临床应用。     

交联透明质素特性的研究

目的 研究交联透明质素的特征和性能,为其应用于临床提供理论依据.方法 采用扫描电镜-能谱仪分析交联透明质素的化学成分.采用傅立叶变换红外光谱法对交联透明质素结构进行分析.使用旋转流变仪测定交联透明质素凝胶的储能模量、耗能模量及粘度等流变学性能的动态变化.采用激光粒度仪测定凝胶中微粒的大小及分布.结果 能谱分析显示交联透明质素中主要成分与天然透明质素相同,但多了硫原子,该元素来自交联剂.红外光谱测定显示,交联透明质素的吸收特征基本与未交联透明质素相似,只是在示硫砜键振动的吸收加强.交联透明质素凝胶的储能模量、耗能模量显著高于天然透明质素凝胶,与频率的依赖性不明显,表现为强凝胶弹性体特征,凝胶微粒平均粒径约为500 μm.结论 交联透明质素是天然透明质素与二乙烯基砜交联反应形成的多聚物,该多聚物溶胀于水后生成水不溶性凝胶.交联透明质素微粒凝胶制剂具有稳定性好和粘弹性高等优点,有利于进一步拓展其临床应用.     

京尼平交联明胶特性随时间变化的研究

研究了京尼平交联明胶材料的交联度、细胞毒性、溶胀度、降解率等特性随交联时间的变化.用1%京尼平交联明胶,按交联时间分为7组:10 min组、30 min组、1 h组、2 h组、12 h组、24 h组、72 h组.结果显示京尼平可有效交联明胶,随着交联时间延长,交联度增加,溶胀度和降解率降低.交联10 min的材料,交联度低(26.7%),溶胀度高(265%),不到一周就完全降解,说明材料很不稳定,易降解;交联30min的材料,交联度(45.7%)、溶胀度(207%)、降解率与10 min材料比都有明显变化,4周未完全降解,8周完全降解,说明京尼平可以在30 min内显著改变明胶性能;30 min后的材料,随交联时间延长,交联度逐渐增加,溶胀度和降解率逐渐降低.交联72 h的材料,交联度73.1%,溶胀度153%,12周仅降解15.6%.MTT法测各组材料细胞增殖率在87.9%～105.4%之间,说明京尼平交联明胶材料细胞毒性均很低.     

低聚乙二醇富马酸酯水凝胶中骨髓来源间充质干细胞的生物学行为

文章快速阅读：  文题释义：水凝胶：是以水为分散介质的凝胶,具有网状交联结构的水溶性高分子中引入一部分疏水基团和亲水残基,亲水残基与水分子结合,将水分子连接在网状内部,而疏水残基遇水膨胀的交联聚合物。是一种高分子网络体系,性质柔软,能保持一定的形状,能吸收大量的水。凡是水溶性或亲水性的高分子,通过一定的化学交联或物理交联,都可以形成水凝胶。这些高分子按其来源可分为天然和合成两大类。天然的亲水性高分子包括多糖类(淀粉、纤维素、海藻酸、透明质酸,壳聚糖等)和多肽类(胶原、聚L-赖氨酸、聚L-谷胺酸等)。合成的亲水高分子包括醇、 丙烯酸及其衍生物类(聚丙烯酸,聚甲基丙烯酸,聚丙烯酰胺,聚N-聚代丙烯酰胺等)。低聚乙二醇富马酸酯水凝胶：是由聚乙二醇和延胡索酸酯与聚乙二醇二丙烯酸酯化学交联而成,具有良好的组织相容性和生物可降解性。实验证明低聚乙二醇富马酸酯水凝胶随着相对分子质量的升高其溶胀度降增加,溶胀比增加引起水凝胶降解速度加快及力学强度减弱。故研制与筛选具有合适溶胀比的低聚乙二醇富马酸酯水凝胶材料是进行骨组织工程支架材料研究的前提。   背景：低聚乙二醇富马酸酯水凝胶是一种具有良好生物相容性及可注射性和可降解性的生物材料。不同相对分子质量水凝胶之间的特性有所差异,将骨髓间充质干细胞包裹其中并诱导细胞成骨分化,相对分子质量相当的水凝胶更有利于细胞增殖和分化,所以采用该材料为骨组织工程支架提供了新的选择。目的：探讨不同相对分子质量的低聚乙二醇富马酸酯水凝胶材料体外包裹大鼠骨髓间充质干细胞的增殖和分化的影响。方法：低聚乙二醇富马酸酯通过氧化还原基团引发系统产生交联,制备出相对分子质量为1000,3000,10000,35 000的低聚乙二醇富马酸酯水凝胶,对水凝胶的溶胀和降解性能进行检测。将骨髓间充质干细胞包裹到4种相对分子质量的水凝胶中,在成骨培养液中诱导1-3周,通过组织学染色(苏木精-伊红染色和茜素红染色)和免疫荧光染色检测水凝胶材料对骨髓间充质干细胞形态的影响以及成骨分化的效果。 结果与结论：①随着水凝胶相对分子质量的增加,成胶时间变短,凝胶的溶胀度明显增加,且随着时间的推移,水凝胶的降解速率与相对分子质量成正比;②细胞复合水凝胶支架材料的组织学与免疫荧光染色结果表明,细胞经过诱导后,在具有适当溶胀与降解特性的相对分子质量为3 000与10 000的水凝胶中所形成的矿化结节数量显著多于在其它两种相对分子质量中的,说明有利于细胞的增殖与分化;③结果表明,低聚乙二醇富马酸酯水凝胶具有良好的生物相容性,且相对分子质量为3 000与     10 000的水凝胶对间充质干细胞的成骨分化有一定的良性调节作用。 中国组织工程研究杂志出版内容重点：生物材料;骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性;组织工程 ORCID:0000-0002-0616-3754(魏丽君)     

不同交联密度甲基丙烯酰酯明胶/脱细胞半月板细胞外基质复合水凝胶的性能

文题释义：脱细胞半月板细胞外基质：由新鲜半月板组织通过湿法差速离心方法脱细胞制备而来,在去除了异体半月板的免疫原性的同时,仍保留了组织的大部分成分和组织本身的生物学性能,例如影响细胞的活性、调控细胞的增殖和分化、促进组织再生和修复等。 甲基丙烯酰酯明胶：是一种明胶的衍生物,由甲基丙烯酸酐与明胶合成而来。在加入光引发剂的基础上经紫外线照射,明胶链上的甲基丙烯酰胺和甲基丙烯酸甲酯侧基聚合形成聚合物链,聚合物链交错纵横,最终形成网状结构。 交联密度：通常用来表征交联聚合物里交联键的数量,主要受交联时间和交联光强度的影响,其可明显改变聚合物链的形成情况。 背景：交联后的聚合物链对水凝胶的基本性质和细胞相容性存在显著影响,而交联密度可明显改变聚合物链的形成情况,有关交联密度对水凝胶性能影响的针对性研究较少。 目的：制备一种细胞相容良好性的复合水凝胶,探究交联密度对该水凝胶性能的影响。 方法：配制甲基丙烯酰酯明胶溶液,然后加入脱细胞半月板细胞外基质溶液与LAP溶液,制备预凝胶溶液,采用蓝光对溶液进行交联,交联时间分别为10,30,60 s,检测3种水凝胶的压缩弹性模量、溶胀倍率与降解性能。将半月板纤维软骨细胞加入预凝胶溶液中,采用蓝光对溶液进行交联,交联时间分别为10,30,60 s,检测培养对应时间的细胞活性、形态与聚集。 结果与结论：①交联60 s水凝胶的压缩模明显高于交联10,30 s的水凝胶(P < 0.05);②交联10 s水凝胶的溶胀倍率明显高于交联30,60 s的水凝胶(P < 0.05),交联30,60 s的水凝胶的溶胀倍率比较差异无显著性意义(P > 0.05);③随着交联时间的延长,水凝胶的降解时间延长,交联60 s的水凝胶需要80 min完全降解,交联10 s的水凝胶50 min就可以完全降解;④培养24 h后,3组水凝胶中的细胞活性均在95%以上,各组之间无差异(P > 0.05);⑤培养1 d时,3组水凝胶中的细胞均呈球形且均匀分布;4 d时,3组细胞均开始伸展,交联10 s水凝胶中有较小的细胞团;7 d时,3组细胞树突状伸展更为明显,其中交联10 s水凝胶中形成了较为明显的细胞团;⑥培养1,7,14 d时,交联10 s水凝胶中的细胞活性均在85%以上。培养1 d时,交联10 s水凝胶中的细胞呈球形分布,且分布均匀;培养28 d时,细胞呈树突状伸展,并聚集形成网状结构;⑦结果表明,甲基丙烯酰酯明胶/脱细胞半月板细胞外基质复合水凝胶的性能可通过调整交联密度进行优化。 ORCID: 0000-0002-3606-1097(周建) 中国组织工程研究杂志出版内容重点：生物材料;骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性;组织工程     

pH敏感性羟乙基甲壳素/聚丙烯酸水凝胶的制备及其释药性能研究

用氯乙醇对甲壳素进行醚化改性，得到水溶性羟乙基甲壳素（Hydroxyethyl chitin，HECH），用化学交联法制备了由聚丙烯酸（PAA）和HECH复合的互穿网络（IPN）水凝胶。溶胀实验表明：该水凝胶在人工肠液（pH7．4，I=0．1）中的溶胀度远大于在人工胃液（pH）．4，I=0．1）中的溶胀度，凝胶的溶胀度随着温度的升高而增大；以该凝胶制备了双氯芬酸钾缓释体系，释放实验表明该凝胶具有较好的缓释性能。     

透明质酸与京尼平的交联研究

对透明质酸与京尼平的交联反应进行初步的探索。透明质酸溶液中加入京尼平进行反应,采用UV 法和粘度法测定交联程度。考察反应时间、京尼平的量、透明质酸的浓度等因素对交联反应的影响。透明质酸与京尼平反应在48 h 时吸光度达到最大值,粘度变化不大。反应时间、京尼平的量及透明质酸的浓度是影响交联的因素,其中反应时间是主要影响因素,为进一步研究透明质酸的交联提供依据。     

碳化二亚胺对透明质酸进行化学修饰的研究

目的:用水溶性的碳化二亚胺(EDC)作为交联剂对透明质酸(HA)进行化学修饰,研究交联产物(HA-EDC)的物化性质和微观结构.方法:制备HA与EDC的交联产物,对该交联产物进行流变性和热学性能的研究,并对其进行红外光谱、拉曼光谱以及核磁共振的微观结构研究.结果:反应时间和交联剂添加量的不同可以改变交联反应的交联度.不同摩尔比的EDC和HA的交联产物有不同的溶胀性.光谱分析得到交联产物的N-酰脲的结构.结论:可以用碳化二亚胺来对透明质酸进行化学修饰,得到具有很强吸水性并且吸水性可以控制的交联产物,交联反应过程中,产物的结构从不稳定的O-异酰脲转变为稳定的N-酰脲.     

人工髓核材料-聚乙烯醇水凝胶的溶胀性能研究

利用冷冻-解冻法制得聚乙烯醇(PVA)水凝胶弹性体,研究了其用于人工髓核材料的溶胀特性,以及聚乙烯醇浓度、溶胀温度、溶胀体系的pH值对其溶胀性能的影响,采用扫描电镜对其微观形貌进行了观察,并对其溶胀动力学进行了探讨。结果表明,聚乙烯醇水凝胶是一种多孔网状结构,网络孔径大小与水凝胶中聚乙烯醇的含量有关;增加聚乙烯醇的浓度,提高溶胀温度以及溶胀体系的pH值,其平衡溶胀率减小;通过溶胀动力学方程对其溶胀过程进行了描述,水凝胶中聚乙烯醇的含量,试样尺寸以及溶胀体系的pH值,是溶胀速率快慢的重要影响因素。     

力学性能可控的甲基丙烯酸化明胶（GelMA）人工软骨性能及软骨/骨表界面结合研究

目的 探寻优化骨表面力学匹配的软骨修复材料,并探讨软骨修复材料与骨结合的表界面性能,为软骨修复材料的设计及制备提供依据。方法 以明胶和甲基丙烯酸酐为原料,在明胶分子中引入双键结构,使用紫外光交联的方式制备甲基丙烯酸化明胶（GelMA）水凝胶。运用扫描电子显微镜、万能力学试验机分析水凝胶的形貌、孔径大小和力学性能;体外降解实验、体外拉伸实验评估水凝胶的降解和拉伸特性;黏附能力定性实验、三维立体光学显微镜和扫描电子显微镜评估水凝胶的黏附能力;细胞增殖测试和Live/Dead染色测定细胞活性和毒性。探讨了不同紫外光照时间下GelMA水凝胶的理化性能及软骨/骨材料结合的表界面结合性能,对性能适宜的修复材料进行了配比、力学优化。结果 紫外光照（UV）交联时间为1 min时水凝胶孔隙最大（110.25±6.51）μm,孔隙率高达（45.24±2.78）%;12 h时水凝胶的平衡溶胀比达（148.43±3.84）%;28 d失重率为（17.40±2.38）wt%;水凝胶的拉伸性能随紫外光照时间延长而逐渐增加;GelMA水凝胶对骨髓间充质干细胞增殖无明显抑制作用;水凝胶软骨修复材料与骨的结合良好,可黏附于仿生骨材料表面。结论 紫外光照交联时间1 min时GelMA水凝胶的吸水速率和平衡溶胀比最佳,降解速快,拉伸性能与天然软骨结构类似,具有良好的生物相容性,与骨修复材料力学匹配,该力学性能可控的GelMA水凝胶软骨修复材料为软骨/骨的连接及性能匹配提供了依据。     

Swelling and Mechanical Properties of Solution‐Crosslinked Poly(isobutylene) Gels

We prepared several series of poly(isobutylene) (PIB) gels starting from butyl rubber in dilute toluene solutions using sulfur monochloride as a crosslinking agent. Solution and suspension‐crosslinking techniques were used for the preparation of PIB gels in the form of rods, membranes, and beads in the size range of 1 to 3 mm. The gels were subjected to dynamic and equilibrium swelling measurements in toluene and cyclohexane as well as to the elasticity tests. Depending on the amounts of sulfur monochloride and butyl rubber in the crosslinking solution, PIB gels with different swelling capacities and elastic moduli were synthesized. The swelling ratio of the gels first increased rapidly with increasing swelling time but then decreased until an equilibrium was obtained. This unusual swelling behavior was accompanied with an increase of the elastic moduli of the gels during the swelling process. The results were explained with the post‐crosslinking reactions taking place during the swelling process of PIB gels. By using the theory of equilibrium swelling, the number of segments in the network chains and the polymer‐solvent interaction parameters were calculated for PIB gels prepared under various reaction conditions.     

Synthesis and characterization of PVNO and PVNO-PVP hydrogels

Hydrogels of crosslinked poly(2-vinylpyridine-1-oxide), PVNO, and poly(2-vinylpyridine-1-oxide)-poly(N-vinylpyrrolidone), PNVO-PVP, were synthesized. PVNO hydrogels were found to be degradable in a pseudo-biological medium and their stability could be increased by increasing the relative concentration of the crosslinking agent. PVNO—PVP hydrogels were also degradable and their stability could be increased by increasing the relative concentration of crosslinking agent and/or poly(N-vinylpyrrolidone). Swelling ratio and percent solvent content for both types of gels were determined. In addition, for PVNO—PVP gels, swelling properties in solvents with different dielectric constants, at different temperatures and ionic strengths were determined. Antibiotic releasing and water vapour adsorption behaviour were also investigated. From the data obtained, the free energy change involved in water vapour adsorption was calculated.     

Innermolekular vernetzte Makromoleküle (Mikrogel): Synthese,Eigenschaften und Nachweis in Gemischen

Emulsionpolymerisation of styrene with divinylbenzene gives spherical particles of nearly uniform diameter, with molecular weights M between 5.10⁵ and 3.10⁷, with various degrees of crosslinking density and with many endgroups. Light scattering, viscosimetry, gelpermeation, diffusion and rheological measurements show, that intramolecular crosslirrking has significant and partly unknown effects: particles of a definite swelling degree have second virial coefficients A₂, which are nearly independent of M. This is contrary to the theory for unsolvated spheres. The FOX -FLORY -constant ? increases with intramolecular crosslinking to the value calculated for spheres, which is larger than that for coils of linear molecules. Gel permeation measures the diameter of the particles in a similar way as hydrodynamics but different from light scattering. At low concentrations the solutions of such gels behave viscosimetrically like dispersions of spheres. At higher concentrations the gel particles deswell and show a concentration dependence of viscosity between that of hard spheres and linear molecules. The viscosity of the solution and its shear dependence are smaller than that of linear molecules, the shear stress τ_w in the turning point of the curves log η vs. log τ being also much smaller. The osmotic pressure at high concentrations is a swelling pressure and depends on the degree of crosslinking. Intramolecular crosslinks reduce the melt viscosity, if the molecular weight of the chain segments between branching points is M_e > 15000. At very high crosslinking densities the melt viscosity is increased. Modulus and density of glassy gel polymers are nearly normal, but the stress at breaking is decreased with increasing intramolecular crosslinking. Particles have higher swelling degrees and intrinsic viscosities as well as lower FOX -FLORY -constants, if they are polymerised in the presence of toluene. Gels cannot be separated quantitatively from linear molecules by sedimentation or by evaluating an abnormal angular dependence of scattered light. But one may calculate the amount of gel and its degree of crosslinking, if one knows the behaviour of linear molecules and measures M_w [η] and a third independent quantity, e.g. melt viscosity, non-NEWTON ian behaviour or the concentration dependence of the viscosity of solutions.     

Reagent-free crosslinking of aqueous gelatin: manufacture and characteristics of gelatin gels irradiated with gamma-ray and electron beam

In order to obtain a gelatin hydrogel crosslinked by a reagent-free method, gamma-ray and electron beam radiation was applied to porcine, bovine and fish gelatin gels and the products were characterized by measuring the gel fraction, the swelling ratio and the enzymatic degradability. On increasing the radiation dose, the gel fraction increased and both the swelling ratio and the enzymatic degradability decreased. The transition temperature from gel to sol of the hydrogel containing more than 5% mammal gelatins increased up to more than 90 degrees C when gamma-ray or electron beam were irradiated by more than 10 kGy. The results show that the degree of crosslinking of irradiated gelatin hydrogels increases with increasing irradiation dose and with decreasing concentration. It is suggested that the radiation crosslinking occurs around the physical crosslinking point or multiple helix structure of gelatin gel.     

Preparation and swelling behavior of chitosan-based superporous hydrogels for gastric retention application

Chitosan and glycol chitosan hydrogels were prepared, and their swelling behaviors in acidic solution were studied to investigate their application for gastric retention device. The optimum preparation condition of superporous hydrogels was obtained from the gelation and blowing kinetics measured at varying acidic conditions. Both the swelling rate and swelling ratio of glycol chitosan hydrogels were higher than those of chitosan hydrogels. Swelling behaviors were significantly affected by not only foaming/drying methods but also crosslinking density, as the sizes and structures of pores generated were highly dependent on those preparation conditions. The prepared superporous hydrogels were highly sensitive to pH of swelling media, and showed reversible swelling and de-swelling behaviors maintaining their mechanical stability. The degradation kinetics in simulated gastric fluid was also studied.     

Photochemical crosslinking improves the physicochemical properties of collagen scaffolds

Collagen is a natural biomaterial with excellent biocompatibility. However, unprocessed collagen has low stability and weak mechanical strength, which limits its application in tissue engineering. The current study aimed to improve the physicochemical properties of collagen scaffolds by using photochemical crosslinking. Collagen gel was reconstituted and photochemically crosslinked by using laser irradiation in the presence of a photosensitizer. Scanning electron microscope was used to characterize the surface and cross-sectional morphology. Stress-strain relationship and other mechanical properties were determined by uniaxial tensile tests. Thermostability and water-binding capacities also were analyzed by using differential scanning calorimetry and swelling ratio measurements, respectively. Photochemically crosslinked porous structures showed fine microstructure with interconnected micron-sized pores, whereas uncrosslinked controls only showed macrosheet-like structures. The stabilizing effect of photochemical crosslinking also was revealed by retaining the three-dimensional lamellae-like structures after thermal analysis in crosslinked membranes but not in the controls. Photochemical crosslinking also significantly reduced the swelling ratio, improved the stress-strain relationship, peak load, ultimate stress, rupture strain, and tangent modulus of collagen membranes. The current study showed that an innovative photochemical crosslinking process was able to produce collagen scaffolds with fine microstructures; to strengthen, stiffen, and stabilize collagen membranes; and to modify their swelling ratio. This may broaden the use of collagen-based scaffolds in tissue engineering, particularly for weight-bearing tissues.     

Quantitative Evaluation of the Swelling and Crosslinking Degrees in Two Organic Gel Packings for SEC

The elution behavior of different solvent/polymer systems has been studied by means of size‐exclusion chromatography (SEC) in two organic column packings based on polystyrene/divinylbenzene (PS/DVB) copolymer, named μ‐styragel and TSK‐Gel H_HR. Both packings offer similar characteristics (pore size, particle size, efficiency) but some differences arise when eluting the same systems. The different elution behavior observed in both polymeric gels has been analyzed in terms of their swelling and crosslinking degrees. To evaluate the magnitude of these parameters, a new equation is proposed which has been derived from the Flory‐Huggins theory of swelling equilibrium, and takes into account binary and ternary thermodynamic interaction functions. The equation appropriately predicts the experimental results, showing that the gel that exhibits more solute‐packing attractive interactions (or adsorption as secondary mechanism) is also the one with the highest crosslinking degree or lowest swelling ratio. In this sense, the μ‐styragel column was the most crosslinked polymer network. In addition, the influence of solvent nature, polymer nature, and solute composition on the observed swelling degree has also been compared in both packings.     

Dynamic behavior of glucose oxidase-containing microparticles of poly(ethylene glycol)-grafted cationic hydrogels in an environment of changing pH

Poly(diethylaminoethyl-g-ethylene glycol) microparticles were prepared by suspension polymerization of diethylaminoethyl methacrylate, poly(ethylene glycol) monomethacrylate and the crosslinking agent tetra(ethylene glycol) dimethacrylate in silicone oil using redox initiators. Particles of different sizes, crosslinking ratios and graft molecular weights were prepared. The changes in the swelling of the particles were studied as the pH was changed between 3.0 and 7.4. The particles showed rapid swelling/deswelling dynamics in response to changes in pH. It was evident that faster response could be obtained from smaller particles. Changing the crosslinking ratio resulted in changes in the extent of swelling, as well as the speed of response. It was also found that longer graft lengths were responsible for increasing the effect of relaxation of the swelling of the network.     

Preparation, characterization, and in vitro enzymatic degradation of chitosan-gelatine hydrogel scaffolds as potential biomaterials

The crosslinking of chitosan (CHT) and gelatin (GEL) accomplished with N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS) was investigated and optimized in relation to hydrogels stability by varying the CHT/GEL mass ratio and the EDC/NHS molar ratio at different and constant EDC concentrations. Hydrogels were also fabricated in the presence of α-tocopherol to assess the release mechanism of a lipophilic drug from a highly-hydrophilic CHT/GEL hydrogel network. Alterations in the physico-chemical properties of hydrogels were characterized by differential scanning calorimetry (DSC) and fourier transform infrared spectroscopy (FTIR), and their biostability was studied within a simulated body-fluid solution (PBS of pH 7.4) at 37 °C for 24 h by evaluating the degree of swelling, followed by topography and morphology characterization using scanning electron microscopy (SEM). The analysis confirmed the formation of a modulated hydrogels porosity using different freezing temperatures prior to lyophilization. The in vitro degradation behaviors of the hydrogels were investigated for up to 5 weeks using collagenase, lysozyme, and N-acetyl-β-D-glucosaminidase by monitoring the weight-losses of hydrogels and their degradation products, being identified by UV-Vis spectroscopy and high-performance liquid chromatography (HPLC) as well as the pH monitoring of degraded solutions. It was observed that an inner morphological hydrogel structure influences their swelling and degradation behavior, which is additionally reduced by in-gel-embedded α-tocopherol because of hydrophobic interactions with their constituents, and hindering the effect on collagenase activity.