中药槐米中芦丁提取工艺研究

目的筛选槐米中提取芦丁的最佳工艺。方法以芦丁提取率为评价指标,采用L_9(3~4)正交实验设计,以乙醇浓度、料液比、提取温度、提取时间为实验因素,确定最佳提取条件。结果最佳提取工艺为乙醇浓度60%,料液比1∶15,提取温度40℃,提取时间30min。结论优选的提取工艺经济、简单、可行,为槐米中芦丁的超声提取提供实验基础。     

槐米中芦丁的超声辅助提取工艺研究

目的研究槐米中芦丁的最佳超声辅助提取工艺。方法在提取功率、超声提取时间、提取次数、料液比等单因素考察试验的基础上,通过正交设计法对超声提取时间、提取温度、料液比进行三因素三水平试验。结果最佳超声辅助提取工艺条件为超声频率20kHz、提取功率800w、提取温度60℃、料液比1：20,超声提取2次,每次30min;芦丁提取率最高为18．25％。结论优选的提取工艺条件简单可行、速度快,可提高槐米中芦丁的收率。     

槐米中芦丁的提取工艺优化及检识

目的 优化槐米的提取工艺以提高芦丁的提取率。方法 在碱提酸沉的基础上,选用电热套加热(130℃,微沸)回流30min,再超声辅助(70℃,40kHz,200W)提取30min的方法提取芦丁。对影响芦丁提取率的因素如pH值、料液比和静置时间进行了考察。应用UV法和HPLC法对自提芦丁进行了检识。结果 最优提取工艺为碱液溶解时pH值为8,料液比为1∶20(g/mL),酸沉淀后静置时间为24h。UV法和HPLC法检识结果表明此法自提的芦丁较纯。结论 优化提取工艺可提高槐米中芦丁的提取率。     

微波法提取全叶马兰总黄酮成分工艺优化

目的：考察影响微波法辅助提取全叶马兰总黄酮的主要因素,用正交实验确定了最优提取工艺。方法：采用微波辅助法进行黄酮类化合物的提取,分析比较料液比例、乙醇浓度提取时间和微波功率对总黄酮提取量的影响,用紫外分光光度法测定黄酮含量。结果：优选了测定波长及显色条件等分析条件,测定方法的回归方程y=0.0213x＋0.0051相关系数R^2=0.9933。单因素实验考察了乙醇浓度、微波功率、提取时间、料液比四个因素对全叶马兰黄酮类化合物提取效率的影响。通过正交试验确定了最佳的提取工艺条件为物料比为1：10、提取时间40s、微波功率406W、乙醇浓度60%。结论：该方法与传统的提取方法相比,提取率高,省溶剂,大大提高了提取效率。     

星点设计-效应面法优选金荞麦提取工艺

目的优选金荞麦中表儿茶素的提取工艺。方法以微波功率、乙醇浓度、料液比、提取时间为自变量,表儿茶素提取率为因变量,通过对自变量各水平的多元线性回归及二项式拟合,用星点设计 效应面法选取最佳工艺,并进行预测分析。结果最佳工艺条件为微波功率211.89 W,乙醇浓度55.11%,料液比1：11.43,提取时间6.16 min,金荞麦中表儿茶素提取率的最大估计值为2.041 4%。实验结果与模型预测值相符。结论该方法简便合理,稳定,可预测性较优,可作为金荞麦的提取工艺。     

微波辅助萃取海芦笋中总黄酮工艺的研究

目的以海芦笋为原料、乙醇为浸提剂,在传统浸提技术基础上,辅以微波提取总黄酮。方法分别考察时间、温度、微波功率、料液比、乙醇浓度对总黄酮得率的影响,并在单因素试验基础上,运用正交试验,确定总黄酮提取最佳工艺条件。结果最佳提取工艺为:提取时间15 min,提取温度60℃,微波功率700 W,料液比1∶40,乙醇浓度55%,在此条件下提取海芦笋干粉中的总黄酮,得率为1.37%。结论微波辅助萃取海芦笋总黄酮的效率高于传统浸提工艺,乙醇浓度、提取时间和温度对总黄酮得率都有显著影响。     

生姜总黄酮的微波-超声辅助协同提取工艺的研究

目的对微波-超声辅助提取生姜总黄酮的工艺条件及参数进行优化。方法在单因素试验的基础上,采用L9(34)正交设计,以生姜总黄酮提取率为指标,试验料液比、提取温度、提取时间、乙醇浓度对提取率的影响。结果最佳提取工艺参数为:提取温度85℃、提取时间60min、乙醇浓度φ=70%、料液比1∶30。结论采用微波-超声辅助协同提取生姜总黄酮工艺简单可行,是提取生姜总黄酮的有效工艺之一。用于提取生姜中的总黄酮,结果满意,回收率为98.4%～101.9%,RSD为1.5%。     

微波辅助萃取马铃薯叶中茄尼醇的工艺研究

目的研究微波辅助萃取马铃薯叶中茄尼醇的工艺条件。方法以95%乙醇为提取溶媒,以反相高效液相色谱法为定量测定方法,考察料液比、辐射时间、微波功率、萃取温度和萃取次数等因素对微波辅助萃取马铃薯叶中茄尼醇提取率和浸膏得率的影响,并与索氏提取法进行了比较。结果微波辅助萃取马铃薯叶中茄尼醇的最佳工艺条件为:料液比1∶10,微波功率450W,萃取温度55℃,提取2次,每次20min。结论应用微波辅助萃取马铃薯叶中的茄尼醇具有萃取时间短,萃取效率高,节约能耗等优点,具有较好的应用前景。     

微波辅助萃取马铃薯叶中茄尼醇的工艺研究

目的 研究微波辅助萃取马铃薯叶中茄尼醇的工艺条件。方法 以95%乙醇为提取溶媒,以反相高效液相色谱法为定量测定方法,考察料液比、辐射时间、微波功率、萃取温度和萃取次数等因素对微波辅助萃取马铃薯叶中茄尼醇提取率和浸膏得率的影响,并与索氏提取法进行了比较。结果 微波辅助萃取马铃薯叶中茄尼醇的最佳工艺条件为：料液比1∶10,微波功率450 W,萃取温度55 ℃,提取2次,每次20 min。结论 应用微波辅助萃取马铃薯叶中的茄尼醇具有萃取时间短,萃取效率高,节约能耗等优点,具有较好的应用前景。     

微波辅助提取雪莲果低聚糖的工艺研究

目的 确立雪莲果低聚糖的微波辅助提取工艺.方法 采用微波辅助提取雪莲果中的低聚糖,以雪莲果低聚糖提取率为考察指标,以提取温度、提取时间、料液比、微波功率、微波时间、解析剂比为考察因素进行正交试验,优化最佳提取工艺条件.结果 最佳提取工艺条件为:提取温度80℃,提取时间60min,液料比50:1,微波功率700W,微波时...     

Optimization of microwave-assisted extraction of solanesol from potato leaves and stems

Microwave-assisted extraction (MAE) was developed for the fast extraction of solanesol from potato leaves and stems. The ratio of raw material to ethanol, extraction time, extraction temperature, and microwave irradiation power were interdependent. The yield of solanesol reached its maximum 98.57% with 1:8.0 g/ml, 40 min, 55°C, and 2.0 KW, respectively. MAE was comparable to other extraction methods, including solvent extraction, heat-reflux, and Soxhlet-extraction. MAE reduced extraction time, solvent consumption, and increased yields of solanesol. Solanesol concentration was performed by RP-high performance liquid chromatography. The method was rapid, simple, accurate, and reproducible.     

中药刺五加中总皂苷的微波辅助提取方法研究

目的:建立中药刺五加中总皂苷微波辅助提取方法。方法:用微波辅助提取法提取中药刺五加中总皂苷。利用正交实验,考察了微波提取条件(包括溶剂、微波辐射时间、提取压力和料液比)对刺五加中总皂苷提取率的影响规律,优化了最佳提取条件。结果:在所选因素水平下,溶剂为70%乙醇、提取压力为700kPa、提取时间为10min、料液比为1:30时,刺五加中总皂苷提取效率最佳。与传统的索氏提取法进行了比较研究,微波辅助提取效率可提高34%。结论:微波辅助提取法提取中药刺五加中总皂苷,提取时间短,提取效率高。     

微波法提取槐花米中的芦丁

目的　优选槐花米中芦丁的最佳提取工艺。方法　以槐花米为原料 ,水为溶剂 ,利用微波辐射提取芦丁。结果　在微波功率为 2 7.2～ 30 .6W的条件下 ,回流提取 (2 4min× 4 ) ,收率 17%。结论　微波加热法提取芦丁 ,方法简便、快速、高效。     

Microwave assisted extraction of curcumin by sample-solvent dual heating mechanism using Taguchi L9 orthogonal design

The present work reports on a novel extraction method using microwaves based on solvent-sample duo-heating synergism, for the extraction of curcumin from Curcuma longa L. The duo-heating mechanism is based on simultaneous heating of sample matrix and extracting solvent under microwave energy. Methanol soaked plant material was used as a modifier to bring about selective and effective heating of the sample under microwave. Acetone was used as the extracting solvent, which has excellent curcumin solubilizing capacity and heats up under microwave owing to its good dissipation factor. Extraction conditions, namely microwave power, irradiation time, particle size and modifier volume were optimized using Taguchi design approach and curcumin was quantified using high performance thin layer chromatography. The optimum conditions as obtained from signal-to-noise ratio analysis and interaction studies between factors were as follows: 20% microwave power, 4 min irradiation time, particles screened through sieve 20 and 8 ml of modifier. Microwave assisted extraction (MAE) under the influence of dual heating mechanism showed better precision and dramatically higher yield with significant reduction in extraction time under optimum extraction conditions, when compared to conventional approaches.     

金银花中黄酮提取工艺研究及野生与人工种植金银花中黄酮含量的比较

目的测定人工种植金银花黄酮的含量和最佳提取工艺。方法以芦丁为对照品,用UV-2450型紫外可见分光光度计测黄酮的含量,通过单因素试验和正交试验,探索微波辐射下溶剂浓度、温度、微波辐射时间、液料比对黄酮提取率的影响。结果人工种植金银花黄酮含量为4.477%,最佳提取工艺条件是：提取温度50℃,料液比1：30,乙醇浓度80%,微波辐射时间45 min。结论人工种植金银花黄酮含量较高,完全满足药用。     

正交实验法优选健胃消食片中原药材微波提取工艺

目的：优选健胃消食片处方中原药材的最佳微波提取工艺。方法：HPLC测定陈皮中的主要成分橙皮苷的含量，以橙皮苷的提取率为指标，L9（3^4）正交实验设计对微波提取工艺的主要影响因素进行了研究。结果：优化的提取工艺为：微波功率为550W，4倍量水，25min／；L，提取两次。结论：优选出的微波最佳提取工艺，提取率高，用于工业化生产前景广阔。     

正交试验优选蔓荆子总黄酮微波辅助提取纯化工艺

目的:优选蔓荆子总黄酮的微波辅助提取纯化工艺。方法:以乙醇浓度、反应温度、微波作用时间、微波功率、料液比为考察因素,以总黄酮含量为指标,通过正交试验优化提取工艺。用D101大孔吸附树脂纯化黄酮粗品。结果:最佳提取工艺为乙醇浓度60%、反应温度80℃、微波作用时间40min、微波功率600W、料液比1∶15;黄酮粗品经纯化后纯度可达84.93%以上。结论:优化后的工艺稳定、可行,可为蔓荆子总黄酮提取纯化工艺的产业化提供理论参考。     

星点设计-响应面法优选藤梨根中总黄酮的提取工艺研究

目的优化藤梨根中总黄酮的提取工艺。方法以微波功率、乙醇浓度、料液比、提取时间为自变量,总黄酮含量为因变量,通过对自变量各水平的多元线性回归及二项式拟合,用星点设计-响应面法选取最佳工艺,并进行预测分析。结果最佳工艺条件为微波功率203.56 W,乙醇浓度80.34%,料液比1∶13.28,提取时间9.78 min,在此最佳条件下,藤梨根中总黄酮含量的最大估计值为103.152 mg.g 1。实验结果与模型预测值相符。结论本方法简便合理、稳定、可预测性较优。     

Intensification of marrubiin concentration by optimization of microwave-assisted (low CO2 yielding) extraction process for Marrubium vulgare using central composite design and antioxidant evaluation

Context: Marrubium vulgare Linn (Lamiaceae) was generally extracted by conventional methods with low yield of marrubiin; these processes were not considered environment friendly.

Objective: This study extracts the whole plant of M. vulgare by microwave assisted extraction (MAE) and optimizes the effect of various extraction parameters on the marrubiin yield by using Central Composite Design (CCD).

Materials and methods: The selected medicinal plant was extracted using ethanol: water (1:1) as solvent by MAE. The plant material was also extracted using a Soxhlet and the various extracts were analyzed by HPTLC to quantify the marrubiin concentration.

Results: The optimized conditions for the microwave-assisted extraction of selected medicinal plant was microwave power of 539?W, irradiation time of 373?s and solvent to drug ratio, 32?mL per g of the drug. The marrubiin concentration in MAE almost doubled relative to the traditional method (0.69?±?0.08 to 1.35?±?0.04%). The IC₅₀ for DPPH was reduced to 66.28?±?0.6?μg/mL as compared to conventional extract (84.14?±?0.7?μg/mL). The scanning electron micrographs of the treated and untreated drug samples further support the results.

Discussion and conclusion: The CCD can be successfully applied to optimize the extraction parameters (MAE) for M. vulgare. Moreover, in terms of environmental impact, the MAE technique could be assumed as a ‘Green approach’ because the MAE approach for extraction of plant released only 92.3?g of CO₂ as compared to 3207.6?g CO₂ using the Soxhlet method of extraction.