| 对照提取物用于金银花药材的质量控制方法研究 |
| |
| 引用本文： | 刘绍勇,张文明,潘建超,薛东升,李文龙,瞿海斌.对照提取物用于金银花药材的质量控制方法研究[J].中国中药杂志,2013,38(13):2147-2150. |
| |
| 作者姓名： | 刘绍勇 张文明 潘建超 薛东升 李文龙 瞿海斌 |
| |
| 作者单位： | 上海凯宝药业股份有限公司, 上海 201401;上海凯宝药业股份有限公司, 上海 201401;上海凯宝药业股份有限公司, 上海 201401;上海凯宝药业股份有限公司, 上海 201401;浙江大学药物信息学研究所, 浙江 杭州 310058;浙江大学药物信息学研究所, 浙江 杭州 310058 |
| |
| 基金项目： | 浙江省公益性技术应用研究计划项目(2012C21102) |
| |
| 摘 要： | 为解决中药材色谱分析中对照品价格较高的问题,该文以金银花药材为例,提出采用对照提取物作为参考物质,对药材进行常规分析的方法。采用对照品对对照提取物进行了标定,测定出其中6种有机酸类化合物的含量,并考察了对照提取物的长期稳定性。利用对照提取物配制不同浓度的溶液,建立了标准曲线。对11批次金银花药材进行分析,采用t检验方法对分别利用对照品和对照提取物测得的结果进行比较,结果表明二者之间不存在显著性差异。该方法可用于金银花药材的质量控制,并对解决类似问题有借鉴意义。
|
| 关 键 词： | 对照提取物 金银花 质量控制 有机酸 |
| 收稿时间： | 2012/12/14 0:00:00 |
Quality control of Lonicerae Japonicae Flos with standard reference extract |
| |
| LIU Shao-yong,ZHANG Wen-ming,PAN Jian-chao,XUE Dong-sheng,LI Wen-long and QU Hai-bin.Quality control of Lonicerae Japonicae Flos with standard reference extract[J].China Journal of Chinese Materia Medica,2013,38(13):2147-2150. |
| |
| Authors: | LIU Shao-yong ZHANG Wen-ming PAN Jian-chao XUE Dong-sheng LI Wen-long and QU Hai-bin |
| |
| Affiliation: | Kaibao Pharmacy Co., Ltd., Shanghai 201401, China;Kaibao Pharmacy Co., Ltd., Shanghai 201401, China;Kaibao Pharmacy Co., Ltd., Shanghai 201401, China;Kaibao Pharmacy Co., Ltd., Shanghai 201401, China;Pharmaceutical Informatics Institute, Zhejiang University, Hangzhou 310058, China;Pharmaceutical Informatics Institute, Zhejiang University, Hangzhou 310058, China |
| |
| Abstract: | The high price of the reference substances is an obstacle for the HPLC analysis of Lonicerae Japonicae Flos. To solve this problem, a new method based on the standard reference extract (SRE) was proposed. In this study, the extract of Lonicerae Japonicae Flos was calibrated, and the long term stability was investigated. Different concentration solutions of SRE were prepared for establishment of the calibration profiles, and 6 organic acids were determined. T-test was used for the comparison of the determination results via reference substances and SRE, and the results demonstrated that there is no significant difference between the two methods. The presented method can be used for the quality control of Lonicerae Japonicae Flos, and will also offer reference to resolve similar problems. |
| |
| Keywords: | standard reference extract (SRE) Lonicerae Japonicae Flos quality control organic acids |
|
|
