| 用SRAP标记分析正品大黄的遗传关系 |
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| 引用本文： | 陈大霞,李隆云,钟国跃,秦松云,王昌华,余再柏.用SRAP标记分析正品大黄的遗传关系[J].中国中药杂志,2008,33(20):2309-2309. |
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| 作者姓名： | 陈大霞 李隆云 钟国跃 秦松云 王昌华 余再柏 |
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| 作者单位： | 重庆市中药研究院,重庆,400065 |
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| 基金项目： | 国家中医药管理局攻关项目 |
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| 摘 要： | 目的：从分子水平上分析3种正品大黄之间的遗传关系。方法：以12个不同来源地的大黄为材料，通过SRAP分析，利用TREECONW软件分析遗传相似系数，UPGMA方法聚类，构建亲缘关系系统图。结果：24对引物组合共得到272条扩增条带，其中有199条呈现多态性，占73.2%，从DNA分子水平显示出供试材料间存在着丰富的遗传多样性，SRAP标记能有效分辨3种正品大黄。遗传相似系数变化范围为0.578 4～0.941 6。聚类分析表明正品大黄的分类结果与传统形态分类结果是一致的。结论：SRAP标记为正品大黄鉴定和遗传关系研究提供了新的DNA分子标记技术手段。
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| 关 键 词： | 大黄 正品大黄 SRAP 遗传关系 |
| 收稿时间： | 1/1/1900 12:00:00 AM |
| 修稿时间： | 1/1/1900 12:00:00 AM |
Study on genetic relationship of official Rheum by SRAP |
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| CHEN Da-xia; LI Long-yun; ZHONG Guo-yue; QIN Song-yun; WANG Chang-hua; YU Zai-bo.Study on genetic relationship of official Rheum by SRAP[J].China Journal of Chinese Materia Medica,2008,33(20):2309-2309. |
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| Authors: | CHEN Da-xia; LI Long-yun; ZHONG Guo-yue; QIN Song-yun; WANG Chang-hua; YU Zai-bo |
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| Abstract: | Objective: To determine the genetic relationship of three species of official Rheum in molecular level. Method: Twelve samples from three species of official Rheum were employed to be analyzed by the approach of sequence-related amplified polymorphism (SRAP). Systematic relationships were constructed based on the UPGMA method by TREECONW software. Result: A total of 272 bands were scored and 199 bands of them were polymorphic, which were up to 73.2% polymorphic ratio. Genetic similarity coefficient was changed from 0.578 4 to 0.941 6.The results indicated that there was abundant genetic diversity among the tested materials. The clustering analysis revealed that the results between SRAP marker and the traditional morphological characteristics was almost the same. Conclusion: SRAP marker is suitable for variety identification and genetic relationship research in official Rheum. |
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| Keywords: | Rheum official Rheum SRAP genetic relationship |
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