加味桃核承气汤对Ⅱ型糖尿病大鼠胰岛素抵抗的影响

目的：观察加味桃核承气汤对Ⅱ型糖尿病（ＮＩＤＤＭ）的影响。方法：采用链脲佐菌素和高热量饲料的方法模拟ＮＩＤＤＭ大鼠模型，研究加味桃核承气汤对ＮＩＤＤＭ大鼠肝细胞膜胰岛素介体释放、脂肪细胞葡萄糖氧化和胰岛素敏感性指数的影响。结果：加味桃核承气汤可明显降低空腹血糖、胰岛素、摄食量和饮水量（Ｐ＜０．０５～０．０１），提高胰岛素敏感性（Ｐ＜０．０５），增加肝细胞膜释放的抑制腺苷酸环化酶活力的胰岛素介体量（Ｐ＜０．０５），提高基础的和胰岛素刺激的大鼠脂肪细胞葡萄糖氧化能力（Ｐ＜０．０５）。结论：加味桃核承气汤治疗可提高ＮＩＤＤＭ大鼠靶细胞对胰岛素的敏感性和反应性，即可使受体和受体后胰岛素抵抗减轻，但不能使之完全逆转。

英文摘要:

To investigate the effect of Supplemented Taohe Chengqi Decoction (STHCQD) in treating non-insulin dependent diabetes mellitus (NIDDM). Methods: The model of rats with NIDDM was formed with injection of streptozotocin and fed on high calorie diet to study the effects of STHCQD on the release of insulin mediator from liver cell membranes, the glucose oxidation in adipocytes as well as the insulin sensitivity. Results: (1 ) Fasting serum glucose, serum insulin, intake of food and water were significantly decreased (P<0. 05～0. 01 ) in STHCQD-treated diabetic rats as compared with untreated diabetic rats, while the insulin sensitivity was significantly increased (P<0. 05 ). (2) The liver cell membranes from STHCQD-treated diabetic rats released the quantity of insulin receptor which inhibited adenylate cyclase activity , but this effect was blunted in untreated diabetic rats(P<0. 05). (3)A significantly increased glucose oxidation in adipocyte of STHCQD-treated diabetic rats was found as compared with those of untreated diabetic rats(P<0. 05). Conclusions:STHCQD therapy increased sensitivity and responsiveness of target cells to insulin, i. e, it might decrease insulin resistance at receptor sites and postreceptor sites in rats with NIDDM, but could not reverse the insulin resistance.

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