世界中医药

目的：观察补肾通督胶囊对肾虚寒凝型类风湿性关节炎患者外周血Th17/Treg细胞及血清白细胞介素17A（IL-17A）、转化生长因子-β1（TGF-β1）、IL-10的影响。方法：入选类风湿性关节炎患者随机双盲分为治疗组（36例）和对照组（35例），治疗组口服补肾通督胶囊，对照组口服雷公藤多苷片，均服药12周，采用流式细胞术观察比较治疗前后两组患者外周血Th17/Treg及采用酶联免疫吸附法（ELISA）观察血清IL-17A、TGF-β1、IL-10的变化。结果：治疗后两组与各自治疗前相比，Th17及Th17/Treg显著降低（P<0.01），Treg显著升高（P<0.01）；治疗后两组间比较无统计学意义（P>0.05）。治疗后两组与各自组治疗前相比，IL-17A显著降低（P<0.01），TGF-β1、IL-10显著升高（P<0.01）；治疗后两组间比较，治疗组TGF-β1与IL-10升高更加明显（P<0.01），IL-17A无统计学意义（P>0.05）。结论：补肾通督胶囊可以明显降低肾虚寒凝型类风湿性关节炎患者外周血Th17/Treg水平，降低血清IL-17A含量，而使血清TGF-β1、IL-10含量升高。

English Summary:

To investigate the effect of Bushen Tongdu capsule on peripheral Th17/Treg ratio and serum IL-17A,TGF-β1 and IL-10 of patients with rheumatoid arthritis(RA).Methods：RA patients were divided randomly and double-blinded into treatment group (n=36) that were treated with Bushen Tongdu capsules and control group (n=35) that were treated with Tripterygium glycosides tablets. Patients in both groups were treated for 12 weeks. The changes of peripheral blood Th17/Treg ratio and serum IL-17A,TGF-β1 and IL-10 were observed by flow cytometry and ELISA in patients of both teams before and after the treatments.Results：After treatments, Peripheral Th 17%，Th17/Treg ratio significantly decreased and Treg% significantly evaluated（P<0.01） in both groups, and there was no statistically significant difference between the two groups after the treatments（P>0.05）. After treatments, the level of serum IL-17A significantly decreased, and TGF-β1 and IL-10 significantly evaluated in both groups（P<0.01）. Comparing the two groups, TGF-β1 and IL-10 evaluated more in treatment group (P<0.01）, while there was no statistically significant difference between the two groups on the changes of IL-17A（P>0.05）.Conclusion：Bushen Tongdu capsule can reduce RA patients` peripheral Th17/Treg ratio and serum IL-17A content, and it also can evaluate serum TGF-β1 and IL-10 content.

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