Abstract:ObjectiveTo study the application and effectiveness of large class problembased learning (PBL) in the prejob training on healthcareassociated infection knowledge for medical students.MethodsMedical students of 2010 session were as the subjects of study, and were randomly divided into control group (n=163) and trial group(n=163). Control group adopted conventional lecturebased learning (LBL), trial group adopted PBL. After finishing the course, two groups of students conducted closedbook exam, and a questionnaire survey was also conducted among students in trial group.ResultsThe score of theoretical exam of trial group was higher than that of control group ([87.99±4.49] vs [81.98±5.73],P=0.001). Large class PBL method can improve students’ active learning enthusiasm, promote initiative research and solving problems, promote communication and cooperation among students, as well as connection between learning and practice.ConclusionLarge class PBL is much better than LBC, but exam system of students’ participation before class needs to be explored constantly during the implementation process.