世界中医药

目的：建立高压液相色谱法(HPLC)同时分析并测定泌感合剂中落新妇苷、野黄芩苷、野黄芩素、木犀草素、芹菜素、异鼠李素的含量。方法：采用Agilent ZORBAX SB-C18色谱柱(4.6×250 mm，5 μm)；流动相：甲醇(A)-0.4%磷酸水（B），梯度洗脱；检测波长320 nm；流速1.0 mL/min；柱温23 ℃。结果：落新妇苷、野黄芩苷、野黄芩素、木犀草素、芹菜素、异鼠李素分别在0.144~1.44 μg（r=0.999 9）、0.196~1.96 μg（r=0.999 7）、0.076~0.76 μg（r=0.999 3）、0.034~0.34 μg（r=0.999 3）、0.032~0.32 μg（r=0.999 8）、0.026~0.26 μg（r=0.999 5）范围内进样量与峰面积线性关系良好；平均回收率在分别为95.6%、96.3%、94.5%、92.3%、97.8%、95.2%；相对标准偏差（RSD）分别为1.7%、1.5%、2.2%、1.2%、2.0%、1.6%。结论：该方法操作简单、稳定、可重复，可用于同时分析测定泌感合剂中6种黄酮类成分的含量。

English Summary:

To develop a method for simultaneous determination of astilbin，scutellarin，scutellarein，luteolin，apigenin，and isorhamnetin in Migan Mixture by high-performance liquid chromatography(HPLC）.Methods:Separation was performed on an Agilent ZORBAX SB-C18 column(4.6 mm×250 mm，5 μm) at 23 ℃.The mobile phases were methanol(A)-0.4% phosphoric acid(B) with gradient elution.The flow rate was 1.0 mL·min-1 and the detection wavelength was 320 nm.Results:Astilbin，scutellarin，scutellarein，luteolin，apigenin，and isorhamnetin showed good linearity between the injection volume and the peak area at 0.144~1.44 μg(r=0.999 9)，0.196~1.96 μg(r=0.999 7)，0.076~0.76 μg(r=0.999 3)，0.034~0.34 μg(r=0.999 3)，0.032~0.32 μg(r=0.999 8)，and 0.026~0.26 μg(r=0.999 5)，respectively.The average recoveries were 95.6%，96.3%，94.5%，92.3%，97.8%，and 95.2%，with RSDs of 1.7%，1.5%，2.2%，1.2%，2.0%，and 1.6%，respectively.Conclusion:This method is simple，stable，and repeatable，which can be used to determine the content of six flavonoids in Migan Mixture by HPLC.

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