| 高浓度葡萄糖、脂肪酸和糖皮质激素对PDX-1表达和胰岛素分泌功能的影响 |
| |
| 引用本文： | 肖常青,邓宏明,黄媛.高浓度葡萄糖、脂肪酸和糖皮质激素对PDX-1表达和胰岛素分泌功能的影响[J].中华内分泌代谢杂志,2006,22(6):590-592. |
| |
| 作者姓名： | 肖常青 邓宏明 黄媛 |
| |
| 作者单位： | 530021,南宁,广西医科大学第一附属医院糖尿病中心 |
| |
| 基金项目： | 广西自然科学基金资助项目（0229027） |
| |
| 摘 要： | SD大鼠胰岛细胞分别在含5.6mmol／L葡萄糖、33mmol／L葡萄糖、0．6mmol／L软脂酸或20nmol／L地塞米松培养液中培养3d，RIA测定基础和葡萄糖刺激后胰岛素分泌量，免疫组化法测定细胞内胰十二指肠同源盒蛋白1（PDX-1）蛋白表达和原位杂交法测定细胞内PDX-1 mRNA表达。结果显示高浓度葡萄糖、脂肪酸和糖皮质激素对PDX-1蛋白表达和胰岛素分泌均有抑制作用。
|
| 关 键 词： | 胰十二指肠同源盒蛋白1 胰岛素分泌 胰岛细胞培养 葡萄糖毒性作用 脂毒性作用 |
| 收稿时间： | 2005-05-30 |
| 修稿时间： | 2005年5月30日 |
Effects of high glucose, fatty acid and glucocorticoid media on PDX-1 expression and insulin secretion |
| |
| XIAO Chang-qing,DENG Hong-ming,HUANG Yuan.Effects of high glucose, fatty acid and glucocorticoid media on PDX-1 expression and insulin secretion[J].Chinese Journal of Endocrinology and Metabolism,2006,22(6):590-592. |
| |
| Authors: | XIAO Chang-qing DENG Hong-ming HUANG Yuan |
| |
| Institution: | The Center of Diabetes, The First Affiliated Hospital, Guangxi Medical University, Nanning 530021 |
| |
| Abstract: | Islet cells isolated from SD rats were incubated separately in mediums containing 5.6 mmol/L glucose,33 mmol/L glucose,20 nmol/L dexamethasone or 0.6 mmol/L palmitate for 3 d.Basal and glucose- induced insulin releases were measured by radioimmunoassay.Pancreatic duodenal homeobox-1 ( PDX-1 ) protein expression was detected using immunochemistry assay.PDX-1 mRNA expression was determined by in situ hybridization.The results showed that the expression of PDX-1 and insulin secretion were depressed by high glucose concentration,dexamethasone or fatty acid. |
| |
| Keywords: | Pancreatic duodenal homeobox-1 Insulin secretion Islet cell culture Glucotoxicity Lipotoxicity |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
