白色念珠菌二相性与口腔致病性关系的实验研究

目的　探讨白色念珠菌二相性与口腔致病性的关系。方法　分别将孢子相、菌丝相白色念珠菌与人口腔颊粘膜细胞 (BEC)进行粘附试验 ,比较二相性白色念珠菌对BEC的粘附率 ;用兔抗人sIgA血清中和人唾液中sIgA ,12 5Ⅰ sIgA放射免疫分析法检测唾液中sIgA的浓度 ,比较含sIgA及不含sIgA的唾液对二相性白色念珠菌粘附BEC的影响。 结果　菌丝相白色念珠菌对BEC的粘附率显著高于孢子相 (P <0 .0 0 1) ;含sIgA的唾液抑制孢子相及菌丝相白色念珠菌粘附BEC的能力强于不含sIgA的唾液 (P <0 .0 0 5 ) ,且唾液中sIgA抑制孢子相白色念珠菌粘附BEC的能力强于抑制菌丝相白色念珠菌粘附BEC的能力 (P <0 .0 5 )。结论　菌丝相白色念珠菌的口腔致病性强于孢子相

Experimental study on relationship between dimorphism of Candida albicans and oral pathogenicity

Objective To study the relation between dimorphism of Candida albicans and oral pathogenicity.Methods Yeast forms and hyphal forms of Candida albicans were used respectively to bind to buccal epithelial cells(BEC) in vitro and compare the adherence rates of hyphal forms with that of yeast forms;Rabbit anti-human serum was used to neutralize salivary sIgA and~(125) I-sIgA radio immuno-assay was used to check the consistency of salivary sIgA for researching the effect of salivary sIgA on the adhesion of dimorphic Candida albicans.Results The adherence of hyphal forms of Candida albicans to BEC was stronger than that of yeast forms(P<0.001);Salivary sIgA could inhibit the adherence of two forms of Candida albicans to BEC(P<0.005)and it was much easier to inhibit the adherence of yeast forms of Candida albicans to BEC(P<0.05).Conclusion The oral pathogenicity of hyphal forms was greater than that of yeast forms in Candida albicans.