血管新生抑制蛋白Vasostatin（120—180aa）防治兔角膜新生血管的研究

目的探讨重组人血管抑制因子Vasostatin（120-180aa）对于碱烧伤诱导的家兔角膜新生血管的抑制作用。方法采用1mol／LNaOH溶液烧伤家兔角膜，建立碱烧伤诱导的家兔角膜新生血管的动物模型；将家兔分为A、B、C、D4组，每组10只眼。伤后24h后分别给予1×PBS缓冲液，20、40、80tc,／mL Vasostatin（120-180aa）球结膜下注射，2次／周，共4周。测量各时间点角膜新生血管的生长面积，观察血管的生长情况。结果各时间点角膜新生血管的面积，B组、c组及D组均低于A组（P〈0．05），C组及D组均低于B组（P〈0．05），C组与D组相比，差异无显著性（P〉0．05）。结论重组人Vasostatin（120—180aa）蛋白可有效抑制角膜新生血管的形成。

Inhibitory effect of recombinant human vasostatin(120-180aa) on rabbit corneal neovascularization

Objective To investigate the inhibitory effect of recombinant human vasostatin (120-180aa) on alkaline-induced corneal neovascularization.Methods Corneal neovascularization animal model was established using 1 mol/L NaOH solution in rabbit.The rabbit eyes were divided into 4 groups:A group (10 eyes),B group (10 eyes),C group (10 eyes) and D group (10 eyes).1×PBS solution buffer,20,40 and 80 μg/mL vasostatin(120-180 aa) protein was subconjunctivally injected respectively in rabbits 24 hours after burning for twice a week.The areas of rabbit corneal neovascularization were measured at each time point.Results The areas of corneal neovascularization in B group,C group and D group were significantly lower than that A group at each time point(P<0.05),and those of C group and D group were lower than B group (P<0.05),but there was not significant difference between C group and D group (P>0.05).Conclusion The recombinant human vasostatin(120-180 aa) can effectively inhibit alkaline-induced corneal neovascularization.