桦木酸对巨噬细胞免疫功能和抗氧化作用的研究

目的为了研究桦木酸对小鼠腹腔巨噬细胞免疫功能和抗氧化作用的影响。方法从白桦树的树皮中提取与合成桦木酸,采用体外培养方法,测定了桦木酸对巨噬细胞能量代谢水平、吞噬能力、胞外一氧化氮(NO)释放量、肿瘤坏死因子(TNF-α)分泌量与谷胱甘肽过氧化物酶(GSH-Px)活性以及胞内超氧化物歧化酶(SOD)与溶菌酶(LSZ)活性的影响。结果桦木酸在2.5～10μg/ml浓度范围内,能够显著性地(P0.05)促进小鼠腹腔巨噬细胞增殖,5～20μg/ml桦木酸能促进巨噬细胞吞噬中性红的能力(P0.05);桦木酸在1.25～10μg/ml浓度范围内,能增强胞外NO的释放量与GSH-Px活性以及胞内SOD与LSZ的活性(P0.05),以1.25μg/ml桦木酸作用最强(P0.01),桦木酸在1.25～20μg/ml浓度范围内,能显著地提高TNF-α的分泌量。结论桦木酸能激活小鼠腹腔巨噬细胞,提高其吞噬能力和能量代谢水平,增强抗氧化能力,提高机体的免疫力。

EFFECTS OF BETULINIC ACID ON IMMUNE FUNCTION AND ANTIOXIDANT ACTIVITY OF MOUSE PERITONEAL MACROPHAGES

Objective To investigate the effects of betulinic acid on immune function and antioxidant activity of mouse peritoneal macrophages.Method The effects of betulinic acid from the bark of white birch on phagocytosis capability,energy metabolism level,nitrous oxide (NO) release,the product of tumor necrosis factor-α (TNF-α),glutathione peroxidase (GSH-Px),superoxide dismutase (SOD) and lysozyme (LSZ) activity were assayed by adding different concentration of betulinic acid into cultured mouse peritoncal macrophages in vitro.Results Compared to the control group,phagocytosis capability was increased at the dose of 2.5-10 μg/ml betulinic acid (P<0.05) and energy metabolism level was significantly enhanced at the dose of 5-20μg/ml betulinic acid (P<0.05),and the NO release,GSH-Px,SOD,LSZ activity were significantly higher at the dose of 1.25-10 μg/ml betulinic acid (P<0.05),and the product of TNF-α was augmented at the dose of 1.25-20 μg/ml betulinic acid.Conclusion Betulinic acid is a possible immune stimulant because it can activate macrophages and enhance its oxidation resistance.