丝裂原活化蛋白激酶p38在急性胰腺炎患者外周血单个核细胞中的变化和意义

目的探讨急性胰腺炎患者外周血单个核细胞(PBMC)内丝裂原活化蛋白激酶p38(p38 MAPK)信号水平的变化及其与疾病严重程度的关系。方法收集轻型胰腺炎(MAP组)29例、重症胰腺炎(SAP组)23例和对照组21例,实时荧光聚合酶链反应检测患者PBMC p38 MAPK基因表达水平,Western blot检测PBMC p38 MAPK和磷酸化p38 MAPK(P-p38 MAPK)蛋白的水平。结果 SAP组p38 MAPK mRNA表达水平和总蛋白水平高于对照组和MAP组(P<0.05);MAP组p38 MAPK mRNA表达水平和总蛋白水平与对照组比较差异无统计学意义(P>0.05)。SAP组P-p38 MAPK总蛋白水平高于对照组和MAP组(P<0.01,P<0.05),MAP组P-p38 MAPK总蛋白水平高于对照组(P<0.05)。结论急性胰腺炎患者PBMC内p38 MAPK mRNA表达水平和总蛋白水平变化较小;p38 MAPK磷酸化水平显著升高,而且与病情程度密切相关。

Significance of mitogen activated protein kinase p38 in peripheral blood mononuclear cells of patients with acute pancreatitis

Objective To investigate the relationship of mitogen activated protein kinase p38(p38 MAPK)levels in peripheral blood mononuclear cell(PBMC) and disease severity in patients with acute pancreatitis(AP). Methods Twenty-nine patients with mild acute pancreatitis(MAP group),twenty-three patients with severe acute pancreatitis(SAP group),and twenty-one healthy volunteers(control group)were evaluated in this study.Levels of p38 MAPK mRNA in PBMC were measured by real-time polymerase chain reaction.Protein levels of p38 MAPK and phosphorylation-p38 MAPK(P-p38 MAPK) were detected by Western blot. Results Levels of p38 MAPK mRNA and p38 MAPK protein in SAP group were higher than control group and MAP group(P<0.05),while there was no difference between MAP group and control group(P>0.05).Levels of P-p38 MAPK protein in SAP group were higher than control group and MAP group(P<0.01,P<0.05);the levels of P-p38 MAPK protein in MAP group were higher than control group(P<0.05). Conclusion It is phosphorylation levels but not total levels of p38 MAPK that significantly increased in PBMC from acute pancreatitis,and P-p38 MAPK takes a close relationship with the grade severity of AP.