| GDNF cDNA在神经及非神经细胞系中的表达 |
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| 引用本文： | 马端端,王晓民,王昕虹,陈光慧,万有,韩济生. GDNF cDNA在神经及非神经细胞系中的表达[J]. 北京大学学报(医学版), 1997, 0(1) |
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| 作者姓名： | 马端端 王晓民 王昕虹 陈光慧 万有 韩济生 |
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| 作者单位： | 北京医科大学神经科学研究中心!100083 |
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| 摘 要： | 目的：研究重组GDNFcDNA在神经及非神经细胞系中的表达。方法：重组克隆，基因转染及RT－PCR。结果：CV1及SH－SY-5Y细胞中无GDNF内源性表达，转基因后出现GDNF表达条带。NG108－15细胞有GDNF的内源性表达，转基因后表达条带增强。结论：重组GDNFcDNA在神经及非神经细胞系中均有较强表达，提示以此制备的工程细胞在Parkinson’s病基因治疗中可能具有重要作用。
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| 关 键 词： | GDNFcDNA 基因转移 聚合酶链反应 Parkinson病／治疗 基因疗法 |
EXPRESSION OF GDNF cDNA IN NERVE AND NON-NERVE CELL LINES |
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| MA Duanduan, WANG Xiaomin, WANG Xinhong, CHEN Guanghui, WAN You, HAN Jisheng. EXPRESSION OF GDNF cDNA IN NERVE AND NON-NERVE CELL LINES[J]. Journal of Peking University. Health sciences, 1997, 0(1) |
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| Authors: | MA Duanduan WANG Xiaomin WANG Xinhong CHEN Guanghui WAN You HAN Jisheng |
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| Abstract: | To study the expression of recombinant GDNF cDNA in nerve and non-nerve celllines. Methods:DNA recombination, gene transfection and RT-PCR. Results: There is no endogenousGDNF expression in CV1 and SH-SY-5Y cell lines. After being trans fected by recombinant GDNF cDNA, the result of RT-PCR shows there appears GDNF expressive band. There is endogenous GDNFexpression in NG-108-15 cell line, after being trans fected by recombinant cDNA, the expression ofGDNF is enhanqed. Conclusion: The recombinant GDNF cDNA can be expressed in nerve and nonnerve cell lines mentioned above, It could be useful in gene therapy for Parkinson's Disease. |
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| Keywords: | GDNF cDNA Gene transfer Polymerase chain reaction Parkinson disease/ther Gene therapy |
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