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Improved PCR for detection of the highly leukotoxic JP2 clone of Actinobacillus actinomycetemcomitans in subgingival plaque samples
Authors:Poulsen Knud  Ennibi Oum-Keltoum  Haubek Dorte
Affiliation:Department of Medical Microbiology and Immunology, University of Aarhus, DK-8000 Aarhus C, Denmark. kp@microbiology.au.dk
Abstract:The JP2 clone of Actinobacillus actinomycetemcomitans is associated with early-onset periodontitis in certain ethnic populations of African origin. Here, we describe and evaluate a set of primers for PCR to assay for the presence of A. actinomycetemcomitans and to discriminate between JP2-like strains and other genotypes in subgingival plaque samples.
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