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TGF-β1对人肝癌细胞系凋亡的调控作用研究
引用本文:王春雷,黄志强,周宁新,Kevin Behrns. TGF-β1对人肝癌细胞系凋亡的调控作用研究[J]. 肿瘤防治研究, 2004, 31(9): 526-528
作者姓名:王春雷  黄志强  周宁新  Kevin Behrns
作者单位:1. 100853,北京,解放军总医院全军肝胆外科研究所
2. Chapel Hill,USA,University of North Carolina
摘    要: 目的 本研究旨在明确人肝癌细胞系中TGF β1的作用及其与凋亡的关系。 方法 本研究选用了 3种含有不同 p5 3基因状态的人肝癌细胞系 ,应用TUNEL技术对TGF β1诱导的肝癌细胞的凋亡进行了定量检测。结果 TGF β1仅能诱导HepG2细胞 (野生型p5 3)凋亡 ,这提示凋亡与 p5 3基因的表达具有明确的联系。结论 HepG2细胞系比Huh 7和Hep3B系细胞更易发生TGF β1诱导的凋亡 ,TGF β1通过p5 3依赖性途径诱导肝癌细胞系发生凋亡

关 键 词:转化生长因子β1  凋亡  肝癌细胞系  细胞培养
文章编号:1000-8578(2004)09-0526-03
收稿时间:2003-08-18
修稿时间:2003-09-27

Study on TGF-β1-Mediated Apoptosis of Human Hepatoma Cell Lines
Kevin Behrns. Study on TGF-β1-Mediated Apoptosis of Human Hepatoma Cell Lines[J]. Cancer Research on Prevention and Treatment, 2004, 31(9): 526-528
Authors:Kevin Behrns
Affiliation:1.Department of hepatobiliary surgery; PLA general hospital; Beijing 100853; China; 2.Chapel Hill; USA; University of North Carolina;
Abstract:Objective The aim of the present study was to determine the relationship between the effect of TGF-β1 and apoptosis in human hepatoma cell lines.Methods Three human hepatic carcinoma cell lines, involving different status of the p53 gene respectively, were used in this study. TGF-β1-induced apoptosis in hepatic carcinoma cell lines was quantitated using TUNEL assay.Results Addition of TGF-β1 induced apoptosis only in HepG2 cells (wild type p53). It was indicated that there is positive correlation between...
Keywords:Transforming growth factor-β1  Apoptosis  Hepatoma cell line  Cell culture
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