| pBBS212-AFP／HSP70真核载体的构建及鉴定 |
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| 引用本文： | 王小平,陈瑞芬,宋爱利,刘彦仿.pBBS212-AFP／HSP70真核载体的构建及鉴定[J].肿瘤研究与临床,2002,14(6):363-365. |
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| 作者姓名： | 王小平 陈瑞芬 宋爱利 刘彦仿 |
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| 作者单位： | 1. 首都医科大学，北京 100054
2. 第四军医大学，陕西 西安 710032 |
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| 摘 要： | 目的 :构建真核表达载体 p BBS2 12 - AFP/ HSP70 ,进行肝癌基因工程疫苗主动免疫的初步研究。方法 :RT- PCR扩增 AFP目的基因片段 ,与同酶切的 p BBS2 12载体连接 ,构建真核表达载体 p BBS2 12 - AFP/ HSP70。用碱裂解法和酶切对筛选阳性克隆进行鉴定。结果 :成功构建p BBS2 12 - AFP/ HSP70真核载体 ,酶切显示 AFP基因片段正确插入载体。结论 :p BBS2 12 - AFP/HSP70真核载体的构建为今后进行基因疫苗的实验研究奠定了良好的基础。
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| 关 键 词： | 重组基因 真核载体 肝癌 |
| 文章编号： | 1006-9801(2002)06-0363-03 |
| 修稿时间： | 2002年9月23日 |
The Construction of an Eukaryotic Expression Vector pBBS212-AFP/HSP70 |
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| WANG Xiao ping,CHEN Rui fen,SONG Ai li,et al..The Construction of an Eukaryotic Expression Vector pBBS212-AFP/HSP70[J].Cancer Research and Clinic,2002,14(6):363-365. |
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| Authors: | WANG Xiao ping CHEN Rui fen SONG Ai li |
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| Institution: | WANG Xiao ping,CHEN Rui fen,SONG Ai li,et al.The Capital University of Medical Sciences |
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| Abstract: | Objective:To study the recombinant polypeptide vaccine active immunotherapy to hepatocarcinoma,an eukaryotic expression vector for AFP gene fragment was constructed.Methods:The gene fragment of AFP with corresponding endonuclease site was amplified by way of RT PCR and recombinated with the pBBS212 vector in the same endonuclease site.Emzymic hydrolysis screened positive clone.Results:Emzymic hydrolysis verified that the human gene AFP was correctly cloned to the vector.Conclusion:The successful constructing eukaryotic vector pBBS212 AFP/HSP70 will lay a better basis for carrying out further studies on the active immunotherapy of recombinant polypeptide and DNA vaccine to hepatocarcinoma. |
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| Keywords: | Recombined gene Eukaryotic vector |
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