| 三氧化二砷负向调控MDSC的肿瘤免疫抑制功能 |
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| 引用本文： | 高清敏,蒋京伟,林浩,周鑫莉,黄若凡,梁晓华.三氧化二砷负向调控MDSC的肿瘤免疫抑制功能[J].中国肿瘤生物治疗杂志,2016,23(2):195-200. |
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| 作者姓名： | 高清敏 蒋京伟 林浩 周鑫莉 黄若凡 梁晓华 |
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| 作者单位： | 复旦大学附属华山医院肿瘤科,上海200040;复旦大学上海医学院肿瘤学系,上海200040 |
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| 基金项目： | 上海市科委自然科学基金资助项目（No.13ZR1405000） |
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| 摘 要： | 目的:研究三氧化二砷(arsenic trioxide,As2O3)是否通过抑制髓系来源抑制性细胞(myeloid-derived suppressor cells,MDSCs)负向调控MDSCs诱导的肿瘤免疫耐受作用.方法:C57BL/6小鼠皮下注射黑素瘤B16细胞和肝癌H22细胞构建移植瘤模型,As2O3处理,观察移植瘤生长情况,流式细胞术检测荷瘤小鼠脾脏内MDSCs和其他免疫细胞的免疫表型,流式细胞术检测2 μmol/L As2O3对B16模型小鼠来源的MDSCs分化的影响.取B16模型小鼠随机分为As2O3处理及对照组,混合淋巴细胞反应检测MDSCs对T细胞免疫抑制活性的改变,ELISA检测B16模型小鼠血清及MDSCs培养上清中TNF-α、IL-10的水平.结果:As2O3抑制B16和H22模型鼠的肿瘤生长,延长B16模型小鼠的生存期,并可显著下调小鼠脾脏内MDSCs的比例.体外经2 μmol/L As2O3处理5d后,B16模型小鼠来源的MDSCs中成熟DCs(CD11c+ CD40+)的比例较对照组显著升高(27.38±4.57)％ vs (17.44±4.51)％,P=0.0078];As2O3组来源的MDSCs对T细胞的免疫抑制活性明显低于对照组(P =0.016);As2O3组小鼠血清及MDSCs培养上清中TNF-α(F=5.78,P=0.014)和IL-10(F=17.83,P=0.045)的含量均较对照组显著降低.结论:As2O3可通过诱导MDSCs向成熟表型分化、下调其免疫抑制活性等,负调控MDSCs的肿瘤免疫抑制功能.
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| 关 键 词： | 三氧化二砷 髓系来源抑制性细胞 B16细胞 H22细胞 |
| 收稿时间： | 2015/9/13 0:00:00 |
| 修稿时间： | 2016/2/22 0:00:00 |
Arsenic trioxide negatively regulating tumor immune suppression function of myeloid-derived suppressor cells |
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| GAO Qingmin,JIANG Jingwei,LIN Hao,ZHOU Xinli,HUANG Ruofan and LIANG Xiaohua.Arsenic trioxide negatively regulating tumor immune suppression function of myeloid-derived suppressor cells[J].Chinese Journal of Cancer Biotherapy,2016,23(2):195-200. |
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| Authors: | GAO Qingmin JIANG Jingwei LIN Hao ZHOU Xinli HUANG Ruofan and LIANG Xiaohua |
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| Institution: | Department of Oncology, Huashan Hospital Affiliated to Fudan University, Shanghai 200040, China;Department of Oncology, Shanghai Medical College of Fudan University, Shanghai 200040, China,Department of Oncology, Huashan Hospital Affiliated to Fudan University, Shanghai 200040, China;Department of Oncology, Shanghai Medical College of Fudan University, Shanghai 200040, China,Department of Oncology, Huashan Hospital Affiliated to Fudan University, Shanghai 200040, China;Department of Oncology, Shanghai Medical College of Fudan University, Shanghai 200040, China,Department of Oncology, Huashan Hospital Affiliated to Fudan University, Shanghai 200040, China;Department of Oncology, Shanghai Medical College of Fudan University, Shanghai 200040, China,Department of Oncology, Huashan Hospital Affiliated to Fudan University, Shanghai 200040, China;Department of Oncology, Shanghai Medical College of Fudan University, Shanghai 200040, China and Department of Oncology, Huashan Hospital Affiliated to Fudan University, Shanghai 200040, China;Department of Oncology, Shanghai Medical College of Fudan University, Shanghai 200040, China |
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| Abstract: | |
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| Keywords: | arsenic trioxide (As2O3) myeloid-derived suppressor cell (MDSC) B16 cell H22 cell |
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