| miR-99b通过下调mTOR的表达抑制胃腺癌细胞系SGC-7901的增殖及迁移 |
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| 引用本文： | 王战豪,潘逼然,张彤彤,郭元彪,张莉萍.miR-99b通过下调mTOR的表达抑制胃腺癌细胞系SGC-7901的增殖及迁移[J].中国肿瘤生物治疗杂志,2016,23(2):230-234. |
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| 作者姓名： | 王战豪 潘逼然 张彤彤 郭元彪 张莉萍 |
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| 作者单位： | 1. 重庆医科大学附属第一医院医学检验科,重庆,400016;2. 重庆医科大学附属成都第二临床学院实验医学科,成都,610031 |
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| 基金项目： | 国家自然科学基金资助项目(No. 81270465)；成都市卫生局计划资助项目(No. 2013059) |
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| 摘 要： | 目的:探讨hsa-miR-99b在胃腺癌细胞系的表达及其影响胃腺癌细胞系增殖和迁移的潜在作用机制.方法:Real-time PCR检测miR-99b在人胃上皮细胞系GES-1和人胃腺癌细胞系SGC-7901、BGC-823、MGC-803中的表达.用人工合成的miR-99b模拟物转染SGC-7901细胞构建过表达miR-996细胞系,转染对照模拟物作为对照组,CCK-8法检测过表达miR-99b的SGC-7901细胞的增殖情况,Transwell法检测SGC-7901细胞系的迁移情况,Western blotting检测SGC-7901细胞系中哺乳动物雷帕霉素靶蛋白(mammalian target of rapamycin,mTOR)的表达情况.结果:miR-99b在胃腺癌细胞系中的表达水平显著低于胃上皮细胞系,其中以SCG-7901细胞最甚.转染miR-99b模拟物48 h后,实验组SCG-7901细胞增殖能力低于对照组(0.20±0.00 vs 0.27±0.02,P＜0.05);与转染对照模拟物的细胞相比,转染24 h后,实验组SCG-7901穿膜细胞数低于对照组(226.67 ±25.17) vs (523.33±25.17)个,P＜0.05].miR-99b可下调人胃腺癌细胞系SGC-7901中mTOR蛋白的表达.结论:miR-99b在胃腺癌细胞系中的表达明显下调,miR-99b抑制人胃腺癌细胞系SGC-7901增殖和迁移的作用可能与下调mTOR表达有关.
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| 关 键 词： | miR-99b 胃腺癌 细胞增殖 细胞迁移 哺乳动物雷帕霉素靶蛋白 |
| 收稿时间： | 2015/12/23 0:00:00 |
| 修稿时间： | 2016/2/24 0:00:00 |
inhibit the proliferation and migration of human gastric adenocarcinoma SGC-7901 cells through down-regulating the expression of mTOR |
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| WANG Zhanhao,PAN Biran,ZHANG Tongtong,GUO Yuanbiao and ZHANG Liping.inhibit the proliferation and migration of human gastric adenocarcinoma SGC-7901 cells through down-regulating the expression of mTOR[J].Chinese Journal of Cancer Biotherapy,2016,23(2):230-234. |
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| Authors: | WANG Zhanhao PAN Biran ZHANG Tongtong GUO Yuanbiao and ZHANG Liping |
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| Institution: | Department of Clinical Laboratory, The First Affiliated Hospital of Chongqing Medical University,Chongqing 400016, China,Department of Laboratory Medicine,The Second Clinical College of Chengdu Affiliated to Chongqing Medical University, Chengdu 610031, Sichuan, China,Department of Laboratory Medicine,The Second Clinical College of Chengdu Affiliated to Chongqing Medical University, Chengdu 610031, Sichuan, China,Department of Laboratory Medicine,The Second Clinical College of Chengdu Affiliated to Chongqing Medical University, Chengdu 610031, Sichuan, China and Department of Clinical Laboratory, The First Affiliated Hospital of Chongqing Medical University,Chongqing 400016, China |
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| Abstract: | |
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| Keywords: | miR-99b gastric adenocarcinoma cell proliferation cell migration mammalian target of rapamycin (mTOR) |
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