大鼠肝移植麻醉与无肝期补液的研究

应用流式细胞分析术研究内毒素对大鼠枯否细胞膜结合型肿瘤坏死因子影响，同时对枯否细胞产生的分泌型肿瘤坏死因子也进行了检测作为对照。结果：正常枯否细胞在无任何刺激时也能表达一定数量（16．6％）的膜结合型肿瘤坏死因子，在受内毒素刺激后经历一明显的下降期后逐步升高．而枯否细胞在受内毒素刺激后分泌型TNF在4h达到高峰，而后逐渐回落。两型肿瘤坏死因子对内毒素的不同的反应性提示二者不同的病理及生理意义．

Experimental study of rat orthotopic liver transplantation: anesthesia and infusion in anhepatic period

This experiment focused on the choice of proper anesthesia drug and its dosage,and the study of the effect of infusion in recipient anhepatic period on various physiological indexes. Methods:We analysed donor and recipient anesthesia procedure, dosage and effect after having performed 150 trans-plants using 300 rats- Eighty SD species rats were divided into two groups, donor and recipient. The latterwas divided into four subgroups, control group C with no infusion in anhepatic peri0d, physical salinegroup G,,NaHCO3 solution group G, and salvia miltiorrhiza solution group G, in dosage of 8 ml/h via supe-rior vena cava catheter in anhepatic period- Infusion begoan at anhepatic period t0 observe changes of physi-ological index. Results: Ketamine dosage of donor analgesia was 100~ 150 mg/kg of choice- Infusion ofsaline, NaHCO3, and salvia miltiorrhiza solution in dosage of 8 ml/h via superior vena cava catheter in an-hepatic period could markedly ameliorate hemokinetics at the end of this period with no significant affectionto body temperature- NaHCO3 could raise pH value compared with control and ameliorate liver functioneven in postoperative reperfusion period while have no obvious effect on PO2, PCO, and SatO23. Salvia mil-tiorrhiza solution could make PO2 higher and PCO2 lower relatively at the end of anhepatic period as well asimprove liver function after reperfusion. Conclusion: Atropine im before recipient operation can markedlyreduce the morbidity of lung infection. Infusion in anhepatic period can c0rrect hemodynamical disorders.Salvia miltiorrhiza solution is of choice.