人参皂甙对烫伤脓毒症大鼠细胞免疫功能的影响

目的：探讨人参皂甙对烫伤脓毒症后细胞免疫功能的影响及其作用机制。方法：健康雄性SD大鼠66只，随机分为脓毒症组（24只）、人参皂甙组（24只）和对照组（18只）。将大鼠用沸水致背部30％总体表面积Ⅲ度烫伤，烫伤后12h腹腔注射内毒素（5mg／kg）予以“二次打击”，制成烫伤脓毒症模型。用流式细胞仪检测大鼠外周血中CD4^＋T细胞、CD8^＋T细胞、CD25^＋T细胞和CD4^＋CD25^＋调节性T细胞（Tr细胞）的细胞数。结果：“二次打击”后大鼠外周血中CD4^＋T细胞、CD25^＋T细胞比例显著下降，CD8^＋T细胞、CD4^＋CD25^＋Tr细胞比例显著升高；此过程随时间而加重，各时间点与对照组比较差异均有显著性（P均〈0．01）；用药后24h、72h占淋巴细胞百分比人参皂甙组CD8^＋T细胞、CD4^＋CD25^＋Tr细胞占淋巴细胞百分比显著低于脓毒症组（P均〈0．01），CD4^＋T细胞、CD25^＋T细胞比例显著高于脓毒症组（P均〈0．01）。结论：烫伤和内毒素“二次打击”后外周血中CD4^＋CD25^＋Tr细胞表达增强，人参皂甙可有效抑制其表达，显著改善烫伤脓毒症时细胞免疫功能障碍。

Effects of ginsenosides on cyto-immunologic function in scalded rats with sepsis

Objective To investigate the mechanism and effects of ginsenosides on cyto-immune(function) in scalded rats with sepsis.Methods Sixty-six male SD rats were randomly divided into scald sepsis group(n=24),ginsenosides group(n=24),and control group(n=18).The model of scald sepsis was(replicated) by scald injury at 30% full-thickness total body surface area and administration of endotoxin((5 mg/kg)) into the peritoneal cavity immediately 12 hours after scald injury.Then the numbers of CD4~+,CD8~+,CD25~+ T cells and CD4~+ CD25~+ regulatory T cells in peripheral blood of rats were counted by flow(cytometry).Results After the second attack,the administration of endotoxin,CD4~+,CD25~+ T cells in(peripheral) blood of rats were decreased significantly and CD8~+,CD4~+ CD25~+ regulatory T cells in(peripheral) blood of rats were increased significantly at all the time points compared with the control group,and(deteriorated) further with the time going on(all P<0.01).The proportions of CD4~+ CD25~+ regulatory T cells in ginsenosides group were significantly lower than those in scald sepsis group at 24 and 72 hours(after)(treatment),and the proportions of CD4~+ and CD25~+ T cells were significantly higher than those in scald(sepsis) group(all P<0.01).Conclusion The expression of CD4~+ CD25~+ regulatory T cells in peripheral blood is(enhanced) after scald injury and administration of endotoxin,while the ginsenosides can effectively(inhibit) the(expression),thus it obviously improve the immune dysfunction in scalded rats with sepsis.