Construction of a recombinant feline herpesvirus type 1 expressing Gag precursor protein of feline immunodeficiency virus |
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| Authors: | E Sato N Yokoyama K Maeda Y Inoshima M Kohmoto Y Ikeda T Miyazawa T Mikami |
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| Institution: | (1) Department of Veterinary Microbiology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan, JP;(2) Department of Microbiology, Faculty of Agriculture, Yamaguchi University, Yamaguchi, Japan, JP |
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| Abstract: | Summary. We constructed a deletion mutant of feline herpesvirus type 1 (FHV-1) and a recombinant FHV-1. The deletion mutant is the
virus with a region (367 bp) deleted from the start codon of thymidine kinase (TK) gene to the SmaI site within the TK gene, and the other is a recombinant FHV-1 expressing Gag protein of feline immunodeficiency virus (FIV),
in which a cDNA encoding the Gag protein of FIV was inserted at the TK deletion site of the former deletion mutant. These
viruses were designated as C7301ddlTK and C7301ddlTK-gag, respectively. Growth kinetics of these viruses in Crandell feline kidney cells was similar to that of the parent C7301
strain. By immunoblot analysis, C7301ddlTK- gag was confirmed to express the FIV Gag precursor protein in the cells.
Accepted November 12, 1997 Received August 26, 1997 |
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