| 脲酶传导速率法测定血清尿素 |
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| 引用本文： | 陈松劲,李红胜.脲酶传导速率法测定血清尿素[J].现代检验医学杂志,2002,17(2):3-4. |
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| 作者姓名： | 陈松劲 李红胜 |
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| 作者单位： | 浙江省嘉兴市第二医院,浙江嘉兴,314000 |
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| 摘 要： | 目的 了解脲酶传导速率法测定血清尿素的实验特点。方法 采用脲酶传导速率法测定血清尿素，并与酶偶联法进行比较。结果 批内精密度为2.67%,2.06%和1.17%，批间精密度分别为3.02%，2.19%和1.82%，线性范围达46.80mmol/L，平均回收率100.71%，脲酶法（Y）与酶偶联法（X）比较：Y=1.0872X-0.4893,r=0.9995，且两者差异无显著意义（P＞0.05)。结论 脲酶传导速率法测定尿素特异、快速、干扰因素少，适合临床应用。
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| 关 键 词： | 脲酶传导速率法 测定 尿素 传导性电极 |
| 文章编号： | 1671-7414(2002)02-003-02 |
| 修稿时间： | 2001年10月15 |
Urease Conductivity Rate method for Detemination of Urea in Serum |
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| CHEN Song jin,LI Hong sheng.Urease Conductivity Rate method for Detemination of Urea in Serum[J].Journal of Modern Laboratory Medicine,2002,17(2):3-4. |
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| Authors: | CHEN Song jin LI Hong sheng |
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| Abstract: | Objective To understand the characteristics of urease conductivity rate method of urea in serum.Methods To apply urease conductivity rate method for determination of urea in serum and to compare it with enzymoanalysis.Results In this method,within run CV s of 2 67%,2 06% and 1.17%;Between run CV s of 3 02%,2 19% and 1 82% The linear range of urea concentration was up to 46 80mmol/L.The mean rate of recovery was 100 71%.Comparison with enzymoanalysis (X) ,yield a correlable equation of Y =1 087 2 X -0 489 3, r =0 999 5, P >0 05.Conclusion This method has advantage of specific reaction and rapid report for clincal application |
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| Keywords: | urea conductivity electrode |
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