罗红霉素对BALB/c小鼠肠道菌群的影响

目的应用PCR-DGGE技术评价罗红霉素对BALB/c小鼠肠道菌群的影响。方法选用SPF级BALB/c小鼠,按5mg/0.1kg每天灌服水溶性罗红霉素10d,停药7d。分别在实验的0d、3d、10d以及停药7d收取每只小鼠粪便,提取基因组DNA,应用PCR-DGGE(聚合酶链式反应——变性梯度凝胶电泳)技术获得肠道菌群分子指纹图谱,进行相似性、多样性分析及优势条带的序列分析。结果 PCR-DGGE可明确将实验0d、3d、10d以及停药7d小鼠分为4个簇,且灌服罗红霉素的小鼠肠道菌群结构多样性明显减少,序列分析表明灌药前后小鼠粪便标本中的拟杆菌和丁酸梭菌分别为优势菌型。结论罗红霉素对BALB/c小鼠肠道菌群有显著影响。

The effects of roxithromycin on the intestinal microbiota of BALB/c mice

Objective To study the effects of roxithromycin on the intestinal microbiota of BALB/c mice by PCR-DGGE technique.Methods Ten BALB/c mice were chosen,water-soluble roxithromycin was given to mice at 5mg/0.1kg of body weight ten days,and stopping drug for 7days.Then the feces were collected at 0d,3d,10d,and natural recovery 7d,respectively.After extraction of bacterial genomic DNA from feces,the diversity,similarity analyses and the sequence analysis of the dominant bands were carried out by DGGE profilings with PCR-DGGE(polymerase chain reaction-denaturing gradient gel electrophoresis) technique.Results The bacterial composition and structure could be divided into four clusters among four periods by PCR-DGGE distinctly,and we found that there were lower bacterial richness in given roxithromycin.Sequence analysis indicated that Bacteroides and Clostridium butyricum were dominant organisms.Conclusion There are significant effects of roxithromycin on the intestinal microbiota of BALB/c mice.