液相色谱-串联质谱法测定人血浆中多潘立酮浓度

目的建立高效液相色谱-串联质谱测定人血浆中多潘立酮(第2代胃动力药)浓度的方法。方法用TC-C18色谱柱,流动相为甲醇(含0.025%甲酸)-1 mmol.L-1甲酸铵溶液(含0.05%甲酸),用梯度洗脱进行分离,流速为1.0 mL.min-1。用正离子化模式,多重反应监测(MRM)扫描方式进行检测定量。结果多潘立酮的线性范围为0.1～50.0 ng.mL-1,日内、日间精密度均小于15%,提取回收率大于80%。结论本方法灵敏、准确、快速,可用于人血浆中多潘立酮浓度的测定和药代动力学研究。

Determination the concentration of domperidone in human plasma by LC - MS/MS

Objective To develop a LC-MS/MS method for the determination the concentration of the domperidone in human plasma.Methods Domperidone and the internal standard were treated by methanol precipitation to remove protein component from human plasma,and then separated on an Agilent TC-C18 column with gradient elution.The mobile phase consisted of methanol(containing 0.025% formic acid)-1 mmol·L-1 ammonium formate solution(containing 0.05% formic acid).The flow rate was 1.0 mL·min-1.Detection was carried out by multiple reaction monitoring on LC-MS/MS system.Results The assay was linear over the range 0.1-50.0 ng·mL-1.Intra-and inter-day precision were both less than 15%,respectively.The recoveries of domperidone were more than 80%.Conclusion It is a rapid,sensitive,selective and reliable method.The assay can be applied for the determination of domperidone in human plasma and the study on pharmacokinetics.