猪血小板衍生生长因子的高效液相色谱分析及对血管内皮细胞DNA合成的影响

目的：测定猪血小板衍生生长因子的相对分子质量和纯度并观察其对培养的人血管内皮细胞DNA合成的影响。方法：用高效液想象以谱凝胶过滤柱，标准蛋白制备标准曲线，测定猪血小板衍生生长因子的相对分子质量和纯度；采用培养的人脐静脉血管内皮细胞，应用^3H-TdR掺入的方法。观察猪血小板衍生生长因子对血管内皮细胞DNA合成的影响。结果：测得的猪血小板衍生生长因子的相对分子质量为29120，纯度为89.46%；猪血小板衍生生长因子可促进处于静止状态的人血管内皮细胞DNA的合成，在40ng/ml的质量浓度时DNA的全盛最为明显，且于血小板衍生生长因子作用48h时合成最为显。结论：猪血小板衍生生长因子的相对分子质量为29120，可明显促进培养的血管内皮细胞DNA的合成。

High performance liquid chromatography analysis of porcine platelet-derived gro wth factor and its effect on DNA synthesis of human vascular endothelial cells

Objective: To determine the molecular weight and purity of porcine platelet derived growth factor (pPDGF) and to investigate its effect on DNA synthesis of human umbilical vein endothelial cells. Methods: In the present experiment, the high performance liquid chromatography was used and the molecular weight and purity of pPDGF were studied. Human umbilical vein endothelial cells was cultured and effects of pPDGF on DNA synthesis of endothelial cells was observed by 3H TdR incorporation in vitro . Results: The findings of high performance liquid chromatography showed that the molecular weight of pPDGF was 29 120 and the purity was 89.46%, and pPDGF significantly promoted DNA synthesis of quiescent endothelial cells with a maximal response at a concentration of 40 ng/ml at 48 h. Conclusion: The molecular weight of pPDGF is 29 120, and it can promote DNA synthesis of cultured human umbilical vein endothelial cells. [