羊膜包裹的自体神经-肌复合移植物桥接大鼠坐骨神经缺损

目的： 探讨羊膜包裹的自体神经-变性骨骼肌复合移植物桥接修复大鼠坐骨神经缺损的可行性。方法：Wistar大鼠54只，分别以羊膜包裹的“神经-肌”并联桥（A组）、自体神经 (B组)和变性骨骼肌桥（C组），桥接10 mm右侧坐骨神经缺损。术后行Fast Blue逆行示踪，神经丝（NF）免疫组织化学和胫前肌湿重、再生纤维数目、直径及髓鞘厚度等检测。结果： A和B组脊髓和背根节荧光标记细胞均多于C组； C组再生神经丝排列稀疏、紊乱，而A和B组稠密、整齐；胫前肌湿重、单位面积再生有髓纤维数目、直径和髓鞘厚度在A与B组比较，差异无显著性(P>0.05)；与C组相比，差异均有显著性(P<0.05)。结论：羊膜包裹的自体神经-肌复合移植物能很好地桥接修复大鼠坐骨神经缺损。

Bridging rat sciatic nerve defects with the composite nerve-muscle autografts wrapped with human amnion matrix membrane

Objective To explore the possibility of bridging the sciatic nerve defects with the composite nerve-muscle (NM) autografts wrapped with human amnion matrix membrane (HAMM) in rats.Methods Fifty-four Wistar rats were divided randomly into 3 groups (n=18), and about 10 mm of right sciatic nerve defects were bridged with the composite NM autografts, the nerve autografts and the denatured skeletal muscle autografts respectively (Group A,B,and C), and all grafts were wrapped with HAMM. After the operation, the regenerated nerves were assessed by Fast blue retrograde tracing, neurofilament (NF) immnohistochemical staining, regenerated axons counting, measuring the diameter and myelin thickness of the regenerated axons, and quantifying the wet weight of tibialis anterior muscle. Results In Group A and B, there were more fluorescent-labeled cells in dorsal root ganglion and spinal cord than in Group C. The regenerated NFs were sparse and disordered in Group C, but dense and regular in Group A and B. Group A and Group B showed results superior to Group C as to other histologic and morphologic characters (P<0.05), whereas there was no significant difference between Group A and B (P> 0.05). Conclusion The composite NM autografts wrapped with HAMM in this study can well bridge and repair sciatic nerve defects in rats.