Changes of NF-κB activity in colon carcinoma cells treated with different crude extracts of abrotani herba

Changes of NF-κB activity in colon carcinoma cells treated with different crude extracts of abrotani herba

Objective  To study changes of NF-κB activity in colon carcinoma cell lines treated with different crude extracts of abrotani herba obtained through solvent extraction methods. Methods  Crude extracts of abrotani herba were extracted with ligarine, chloroform, acetoacetate and n-butanol in separating funnel. Exposure concentration of crude extracts were obtained through detecting viability of HT-29 cells by MTT. Then HT-29 cells and Lovo cells were treated with different crude extracts respectively. Changes of NF-κB activity in HT-29 cells and Lovo cells using different crude extracts were observed by EMSA. Results  Successfully extracted different crude extracts of abrotani herba and called them ligarine extract, chloroform extract, acetoacetate extract, n-butanol extract and remaining extract for short. NF-κB activity was significantly inhibited in HT-29 cells treated with chloroform extract, there were no significant differences in other groups compared with the control. The same change of NF-κB activity was observed in Lovo cells using different crude extracts of abrotani herba. Conclusion  NF-κB activity can be inhibited in colon carcinoma HT-29 cells and Lovo cells treated with chloroform extract obtained from abrotani herba through the method of solvent extraction. Supported by a grant from the Project of Tackling Key Problems in Science and Technology Foundation of Chongqing (No. 9398).