细胞外Ca^2＋浓度对培养腮腺腺细胞分化和功能的影响

为研究高于生理浓度的细胞外Ｃａ２＋对腮腺腺细胞分化和分泌功能的影响，以１０倍和２０倍生理浓度的Ｃａ２＋作用于培养中的腮腺腺细胞，应用免疫组织化学技术，观察细胞外Ｃａ２＋对细胞连接蛋白及与分化有关的蛋白表达的影响。结果显示，细胞粘接分子（ＣＡＭ）、缝隙连接蛋白（Ｃｘ３２）随细胞外Ｃａ２＋升高而表达升高；ｃｊｕｎ蛋白随Ｃａ２＋浓度增高表达更广泛；而表皮生长因子受体（ＥＧＦγ），ｃｆｏｓ及腮腺特异性分泌蛋白α淀粉酶、富含脯氨酸蛋白（ＰＲＰ）表达无明显变化。研究结果提示，细胞外Ｃａ２＋在一定程度上可促进细胞分化，但对细胞分泌功能无明显影响。

The role of calcium on parotid cell differentiation and secretion in vitro

To study the role of calcium on parotid cell differentiation and secretion, high extracellular calcium was used to treat the parotid gland cells in culture. Immunohistochemical staining was applied to examine the expression of proteins involved in cell-cell interaction, cell differentiation and parotid gland secretary proteins. The results demonstrated that the expression of CAM and Cx32 increased and more cells had positive reaction to c-jun oncoprotein. No effect of calcium on the expression of EGF-gamma, c-fos, alpha-amylase and PRP was observed. The data indicate that extracellular calcium play an important role on cells differentiation. However, it does not affect the cell secretion.