硒对糖尿病大鼠肝脏磷脂酶D基因表达的影响

目的:研究硒对糖尿病大鼠代谢相关基因表达的调控作用。方法:对前期研究分离到的与补硒50μg/kgbwd有关的糖尿病大鼠差异显示的基因片段进行克隆、测序及同源性分析,选择目的基因进行RT-PCR验证,以观察各组大鼠之间肝脏中基因表达的变化。结果:差异显示基因片段Se-4的碱基序列与磷脂酶D(PLD)基因片段的同源性为100%。RT-PCR验证结果显示:PLDmRNA在糖尿病对照组、糖尿病补硒组大鼠肝脏中的表达水平较正常对照组明显增高,但糖尿病补硒组PLDmRNA的表达较糖尿病对照组有所降低(P<0.05)。结论:硒可抑制糖尿病大鼠肝脏中PLDmRMA的表达,这可能是硒改善糖尿病糖、脂代谢紊乱的分子机制之一。

EFFECTS OF SELENIUM ON GENE EXPRESSION OF PHOSPHOLIPASE D GENE IN LIVER IN DIABETIC RATS

Objective: To study the regulation of selenium on gene expression of phospholipase D (PLD) gene in relation to liver metabolism of diabetic (DM) rats. Method: Differential display cDNA fragments were isolated from former research project in diabetic rats which were supplemented orally with 50 g/kg bw d selenium for 60d, and were cloned, sequenced, and the homology was analyzed. RT-PCR was made using the primers designed according to the sequences of cDNA fragments. Results: In differential display cDNA fragment, Se-4 and PLD were homologous with sequence identities of 100%.The expression levels of PLD mRNA in DM group and DM Se group was higher obviously than that of NC group(P<0.05). The expression levels of PLD mRNA in DM Se group were lower than those of DM group (P<0.05). Conclusion: Selenium supplementation could inhibit the expression of PLD mRNA in liver of diabetic rats. It may be one of the molecular mechanisms that selenium can improve the disorders of glucose and lipids metabolism in diabetic rats.