复方岗梅合剂的制备及质量控制

目的:制备复方岗梅合剂并建立其质量控制方法。方法:以广东土牛膝、岗梅根、甘草制备复方岗梅合剂;采用薄层色谱法对其中的岗梅根、甘草进行定性鉴别;用高效液相色谱法测定其中甘草酸的含量。结果:制得的合剂为棕黑色溶液;薄层色谱对岗梅根、甘草特征斑点定性鉴别清晰,供试品色谱中,在与对照药材色谱相应的位置上显相同的荧光斑点;甘草酸进样量在3.6μg～21.6μg范围内与峰面积积分值线性关系良好,平均回收率为99.95%(RSD=1.62%)。结论:本制剂制备工艺简单,质量控制方法简便、准确、重现性好。

Preparation and Quality Control of Compound Roughhaired Holly Mixture

OBJECTIVE:To prepare Compound Roughhaired Holly Mixture and establish its quality control standards.METHODS:Qualititive identification of the principal agents like Roughhaired Holly root and glycyrrihizic were performed by TLC ;The content of glycyrrihizic acid was determined by HPLC.RESULTS:The preparation was brownish-black.The spots were clear and centralized,The linear range was 3.6μg～21.6μg;the average recovery was 99.95%(RSD=1.62%).CONCLUSIONS:The preparing technique of Compound Roughhaired Holly Mixture was simple;the quality control method was stable,accurate and feasible.