| 库拉索芦荟多糖纯化方法研究 |
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| 引用本文： | 陈伟,陈俊,林新华,黄丽英,罗红斌.库拉索芦荟多糖纯化方法研究[J].药物生物技术,2004,11(3):184-186. |
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| 作者姓名： | 陈伟 陈俊 林新华 黄丽英 罗红斌 |
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| 作者单位： | 福建医科大学药学系,福建,福州,350004 |
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| 基金项目： | 福建省科技厅重点攻关课题 ( 2 0 0 2 10 15 ) |
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| 摘 要： | 醇沉法提取库拉索芦荟粗多糖，分别用分部醇沉法和SephadexG-100凝胶柱色谱纯化粗多糖。结果表明经Sephade。G-100凝胶柱色谱分离纯化可得到均一的库拉索芦荟多糖。经苯酚-硫酸法测定多糖含量为81．11％。在sephadex G-100凝胶柱色谱纯化中用蒸馏水为洗脱液，紫外法200nm检测，方法简便、快速、灵敏度高。
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| 关 键 词： | 库拉索芦荟 多糖 纯化 |
| 文章编号： | 1005-8915(2004)03-0184-03 |
Study on the Purification of the Polysaccharide from Aloe Barbadensis Miller |
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| CHEN Wei,CHEN Jun,LIN Xin-hua,HUANG Li-ying,LUO Hong-bin.Study on the Purification of the Polysaccharide from Aloe Barbadensis Miller[J].Pharmaceutical Biotechnology,2004,11(3):184-186. |
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| Authors: | CHEN Wei CHEN Jun LIN Xin-hua HUANG Li-ying LUO Hong-bin |
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| Abstract: | The polysaccharide from Aloe barbadensis Miller was precipitated with ethanol and further purified by stepwise precipitation or on Sephadex G-100 column. The results showed that crude polysaccharide could be purified on Sephadex G-100 column. Its polysaccharide content was 81.11% detected colorimetrically by the phenol-sulfuric acid method. The polysaccharide was eluted with distilled water and detected by ultraviolet spectrophotography at 200()nm when purified on Sephadex G-100 column.This method was convenient, rapid and sensitive. |
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| Keywords: | Aloe barbadensis Miller Polysaccharide Purification |
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