| 眼镜蛇毒细胞毒素CTX-d的分离纯化及抗肿瘤活性 |
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| 引用本文： | 陈纯,陈崇宏. 眼镜蛇毒细胞毒素CTX-d的分离纯化及抗肿瘤活性[J]. 海峡药学, 2007, 19(3): 38-41 |
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| 作者姓名： | 陈纯 陈崇宏 |
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| 作者单位： | 福建医科大学药学院药理系,福州,350004;福建医科大学药学院药理系,福州,350004 |
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| 摘 要： | 目的 分离纯化眼镜蛇毒细胞毒素,测定其体内外抗癌作用.方法 应用柱层析及RP-HPLC,从眼镜蛇毒粗毒中分离纯化细胞素素(CTX).体内外抗癌作用利用噻唑兰(MTT)法及对荷瘤小鼠U14瘤的抑瘤作用.结果 分离纯化获得的CTX-d不混有PLA2,在体内外实验中显示明显的抗肿瘤作用.结论 结合阳离子交换层析和RP-HPLC可从眼镜蛇毒中高效分离获得不含PLA2的CTX.
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| 关 键 词： | 眼镜蛇毒 细胞毒素(CTX-d) 分离纯化 抗肿瘤活性 |
| 文章编号： | 1006-3765（2007）03-0038-04 |
Purification and antitumor activity of CTX-d from Naja atro venom |
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| CHEN Chun,CHEN Chong-hong. Purification and antitumor activity of CTX-d from Naja atro venom[J]. Strait Pharmaceutical Journal, 2007, 19(3): 38-41 |
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| Authors: | CHEN Chun CHEN Chong-hong |
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| Affiliation: | Department of Pharmacology, Fujian Medical University, Fuzhou 350004, China |
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| Abstract: | OBJECTIVE To purify cytotoxin(CTX) from Naja naja atra venom,and to study its antitumor activity.METHODS CTX-d was fractionated and purified from Naja naja atra venom by column chromatography and RP-HPLC.MTT was used to detect the antitumor effect of CTX-d in vitro,in vivo U14 bearing mice was used to test the effects of CTX-d. The purified CTX-d without PLA2 showed potent antitumor activity both in vivo and in vitro.CONCLUSION The method of successive cation-exchange and RP-HPLC can efficiently purify CTX without PLA2 from Naja naja atra venom. |
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| Keywords: | Cytotoxin(CTX) Purification Antitumor activity |
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