Copaiba oil suppresses inflammation in asthmatic lungs of BALB/c mice induced with ovalbumin |
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| Affiliation: | 1. Faculty of Pharmacy, Department of Pharmaceutical Sciences, Federal University of Juiz de Fora, R. José Lourenço Kelmer s/n, Campus Universitário, 36036-900 Juiz de Fora, MG, Brazil;2. Laboratory of Experimental Immunology and Pathology, Reproduction Biology Center (CBR), Federal University of Juiz de Fora, R. José Lourenço Kelmer s/n, Campus Universitário, 36036-900 Juiz de Fora, MG, Brazil;3. Department of Chemistry, Faculty of Philosophy, Sciences and Letters of Ribeirão Preto, University of São Paulo, Av. Bandeirantesn° 3900, 14040-901 Ribeirão Preto, SP, Brazil;4. Health Department, Faculty of Medical Sciences and Health of Juiz de Fora (SUPREMA), Alameda Salvaterra n° 200, Salvaterra, 36.033-003 Juiz de Fora, MG, Brazil;1. School of Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, People׳s Republic of China;2. Key Laboratory of Structure-Based Drug Design & Discovery Ministry of Education, Shenyang Pharmaceutical University, Shenyang 110016, People׳s Republic of China;3. Zhejiang Respiratory Drugs Research Laboratory of State Food and Drug Administration of China, Medical College of Zhejiang University, Hangzhou 310058, People׳s Republic of China;4. Liaoning Province Institute of Pharmaceutical Research, Shenyang 110015, People׳s Republic of China;1. Pharmacology Area, Department of Basic Sciences in Health, Faculty of Medicine, Federal University of Mato Grosso, UFMT, 78060-900 Cuiabá, MT, Brazil;2. Physiology Area, Department of Basic Sciences in Health, Faculty of Medicine, Federal University of Mato Grosso, UFMT, 78060-900 Cuiabá, MT, Brazil;3. Histology and Cell Biology Area, Department of Basic Sciences in Health, Faculty of Medicine, Federal University of Mato Grosso, UFMT, 78060-900 Cuiabá, MT, Brazil;4. Pharmacy Graduation Curse, Faculty of the Northwest of Mato Grosso, Juína Association of Higher Education, AJES, 78320-000 Juína, MT, Brazil;5. Laboratory of Natural Research Products, Faculty of Medicine, Federal University of Tocantins, UFT, 77020-210 Palmas, TO, Brazil;6. Post-Graduate Studies in Agroenergy, Federal University of Tocantins, UFT, 77020-210 Palmas, TO, Brazil;1. Universidade de Franca, Avenida Dr. Armando Salles Oliveira, 201—Parque Universitário, 14404-600 Franca, São Paulo, Brazil;2. Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Avenida do Café S/N, 14040-903 Ribeirão Preto, São Paulo, Brazil;1. Departamento de Biologia Animal, Instituto de Biologia, Universidade Estadual de Campinas, Campinas, São Paulo, Brazil;2. Departamento de Bioquimica e Biologia Estrutural, Instituto de Biologia, Universidade Estadual de Campinas, Campinas, São Paulo, Brazil;3. Faculdade de Farmácia, Universidade Federal de Juiz de Fora, Minas Gerais, Brazil;4. Departamento de Medicina Tropical, Universidade Federal de Pernanbuco, Pernanbuco, Brazil;1. Univ. Grenoble Alpes, LGP2, F-38000 Grenoble, France;2. Federal University of São Carlos (UFSCar), São Carlos, SP, Brazil;3. University of São Paulo (USP), São Carlos, SP, Brazil |
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| Abstract: | Asthma is a chronic inflammatory disease that represents high hospitalizations and deaths in world. Copaiba oil (CO) is popularly used for relieving asthma symptoms and has already been shown to be effective in many inflammation models. This study aimed to investigate the immunomodulatory relationship of CO in ovalbumin (OVA)-induced allergic asthma. The composition of CO sample analyzed by GC and GC–MS and the toxicity test was performed in mice at doses of 50 or 100 mg/kg (by gavage). After, the experimental model of allergic asthma was induced with OVA and mice were orally treated with CO in two pre-established doses. The inflammatory infiltrate was evaluated in bronchoalveolar lavage fluid (BALF), while cytokines (IL-4, IL-5, IL-17, IFN-γ, TNF-α), IgE antibody and nitric oxide (NO) production was evaluated in BALF and lung homogenate (LH) of mice, together with the histology and histomorphometry of the lung tissue. CO significantly attenuated the number of inflammatory cells in BALF, suppressing NO production and reducing the response mediated by TH2 and TH17 (T helper) cells in both BALF and LH. Histopathological and histomorphometric analysis confirmed that CO significantly reduced the numbers of inflammatory infiltrate in the lung tissue, including in the parenchyma area. Our results indicate that CO has an effective in vivo antiasthmatic effect. |
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