极限门静脉结扎后增生肝脏组织中NF-κB和IL-6表达变化的实验研究

目的:探讨核转录因子(Nuclear factor-kappa B,NF-κB)和白介素-6(Interleukin 6,IL-6)在90%极限门静脉分支结扎大鼠增生肝脏组织中的表达及其与肝再生的关系.方法:96只Sprague-Dawley(SD)雄性大鼠随机分成假手术对照组和门静脉结扎实验组.观察术后0.5、1、3、5、7、14、21和28 d保留侧肝脏重量变化,光学显微镜下观察保留侧肝细胞的形态变化,用免疫组化方法检测保留侧肝细胞的增殖细胞核抗原(Proliferating cell nuclear antigen,PCNA)及NF-κB和IL-6的表达,并进行统计分析.结果:①90%极限门静脉分支结扎后,结扎侧肝叶呈进行性萎缩变小,保留侧肝叶占总肝质重的比例与假手术对照组比较1-5 d升高,7 d达"平台期";②PCNA阳性细胞计数与假手术对照组比较,0.5-7 d表达增强(P<0.05),以后逐渐恢复正常;③保留侧肝叶NF-κB和IL-6的表达量与假手术对照组比较0.5-5 d表达增强,术后7-28 d差异无统计学意义(P>0.05);保留侧肝叶IL-6的表达量0.5d开始呈显著增加,并迅速达到高峰,随后1-5 d表达逐渐下降,但仍高于正常水平,术后7～28 d与对照组比较差异无统计学意义(P>0.05).④大鼠肝脏PCNA的表达在术后0.5、1、14、21、28 d组与肝内NF-KB表达呈正相关,在术后1、14、21 d组与肝内IL-6表达呈正相关,NF-K B的表达在术后1、7、21、28d组与肝脏IL-6表达呈正相关.结论:NF-κ B和IL-6蛋白在90%极限门静脉分支结扎后大鼠肝脏再生过程中发挥重要作用.

A study on expression of nuclear factory-kappa B and IL-6 after 90% portal vein ligation in rats

Objective: To evaluate the expression of NF- κB and IL-6 and its relationship with liver regenerationafter 90% portal branch ligation in rats. Methods:Ninety-six SD rats were randomly divided into Sham-PBL group and portal vein branches ligation group.The weight of both ligated and unligated lobes of liver were measured at POD 0.5,1,3,5,7,14,21 and 28. The morphological changes of the non-ligated liver lobes were observed by microscope.The expression of PCNA, NF- κB and IL-6 of the non-ligated liver lobes were studied with immunohistochemistry. The results were analyzed by medical statistics methods. Results:①Hepatic lobe at the ligated side diminished progressively after 90% portal branch ligation.The ratio of non-ligated lobes weight to the whole liver increased at POD 1-5 period, and got "the plateau stage"at POD7; ②PCNA index were increased at POD 0.5-7 (P<0.05), then gradually return to normal lately;③The expression of NF- κB and IL-6 of the non-ligated liver lobes were increased at PODO.5-5,then gradually return to normal in POD7-28;④The PCNA and NF-κB in liver had a positive correlation at POD0.5,1,14,21,28.The expressions of PCNA and IL-6 had a positive correlation at POD1,14,21.The expressions of NF-κ B and IL-6 had a positive correlation at POD1,7,21,28. Conclusion:The expression of NF-κB and IL-6 may has played a pivotal role in liver regeneration after 90% portal branch ligation in rats.