bFGF、VEGF在大鼠创伤海马脑组织中的表达

目的 研究碱性成纤维细胞生长因子(bFGF)和血管内皮细胞生长因子(VEGF)在大鼠创伤海马组织中不同时间的表达及其它们之间的关系，从分子水平探讨颅脑损伤后的病理机制，为临床治疗脑损伤的新途径提供实验基础。方法 改进Marmarou大鼠加速弥漫性脑损伤模型，取海马区创伤脑组织免疫组化染色观察bFGF、VEGF基因表达情况。结果 海马CAl区伤后6h，bFGF基因表达增加，12h达高峰；VEGF基因表达伤后24h达高峰，72h回复到对照水平。结论 bFGF、VEGF基因表达与脑损伤密切相关，作为生长因子，bFGF、VEGF可能参与颅脑损伤后神经元保护及损伤后修复过程。

The expression of bFGF and VEGF in rat hippocampal following brain injury

Objective To study the expression and relationship of bFGF and VEGF in hippocampal CAl sectors at different time in a rat model of traumatic brain injury (TBI) and to investigate the pathogenic mechanism of brain injury molecularly. Methods Impact-acceleration injury was produced in Wistar rats by the method described by Marmarou with some modificatioa Paraffin-embedded coronal sections of hippocampal CAl in these rats were analyzed immunohistochemically for bFGF and VEGF. Results The expression of bFGF mRNA began to increase 6 h after injury and rose to the climax in 12 h and 24 h after injury respectively for bFGF and VEGF which returned to baseline in 24 h. Conclusion These findings show a close relationship between bFGF or VEGF gene expression and the degree of brain injury. bFGF and VEGF, as growth factors, have a significant protective effect against brain injury following traumatic brain injury.