聚二乙醇酸与Degrapol对肌成纤维细胞粘附、生长的影响

目的　寻找一种更加理想的人工降解材料 ,为改进人工降解材料提供理论依据。方法　比较聚二乙醇酸( PGA)与 Degrapol对肌成纤维细胞粘附、生长、繁殖及产生胶原的影响。结果　 1细胞种植 2周和 6周 ,PGA组的细胞计数明显多于 Degrapol组 ( P<0 .0 1) ;2 6周 PGA组胶原含量明显高于 Degrapol组 ( P<0 .0 1) ;3 2周肌成纤维细胞粘附于 PGA上 ,分布广泛 ;6周后 PGA降解 ,胶原含量丰富。而 Degrapol则部分降解 ,胶原含量较少。结论　Degrapol作为一种新型人工降解材料 ,若用于构建组织工程心脏瓣膜 ,在细胞亲和力等方面尚有待进一步改进

Evaluation of Biodegradable Meshes: Polyglycolic Acid and Degrapol

Objective To search for a more perfect artificial degradable material. Methods The effects of polyglycolic acid (PGA) and Degrapol on cell adhesion, growth, reproduction and collagen synthesis were compared. Results The cell numbers of attachment and growth in PGA group were much more than in Degrapol group after seeding for 2, 6 weeks (P<0.01). The hydroxyproline contents in PGA group were higher than in Degrapol group after seeding for 6 weeks (P<0.01). The microscopic examinations showed more myofibroblasts attached and distributed on PGA fiber after seeding for 2 weeks. PGA meshes were degraded and tissue engineered heart valves were created with sufficient collagen after seeding for 6 weeks, but Degrapol meshes were partly degraded with insufficient collagen. Conclusion Degrapol, as a new kind of degradable meshes, should be improved on cell affinity.