内毒素经氢氧化钙处理后免疫原性改变的研究

目的观察Ca(OH)2作用前后内毒素(lipopolysaccharide, LPS)免疫原性的改变;并利用ThP-1单核细胞系建立体外培养模型,为进一步的研究提供实验基础.方法利用ThP-1单核细胞系建立体外培养模型,以肿瘤坏死因子α(TNF-α)的产量作为衡量刺激物免疫原性的指标,观察Ca(OH)2作用前后LPS免疫原性的变化.结果在观察的LPS浓度范围内(10 μg/L～10 mg/L),与Ca(OH)2作用1周后,LPS免疫原性较对照组明显减弱(P<0.001);随pH值升高,TNF-α的产量呈下降趋势,pH≥12.30时,与对照组相比差异无显著性(P>0.05).结论 LPS与Ca(OH)2作用一周后,免疫原性明显减弱.Ca(OH)2的高pH值在LPS的免疫原性改变过程中起重要作用.

Alteration of immunological properties of bacterial lipopolysaccharide by calcium hydroxide treatment

OBJECTIVE: To study alteration of immunological properties of LPS by Ca(OH)2 treatment, and to establish an experimental model in which ThP-1 cell line was cultured in vitro to observe the alteration of immunological properties of LPS. METHODS: ThP-1 cell line was used to establish a culture model in vitro. Amount of TNF-alpha released from stimulated cells acted as the index on immunological properties of the stimuli and alteration of immunological properties of LPS by Ca(OH)2 treatment. RESULTS: Within the range of observed LPS concentrations (10 micrograms/L-10 mg/L), the original immunological properties of LPS decreased significantly after treatment by Ca(OH)2 for a week (P < 0.001). When pH > or = 12.30, the amount of TNF-alpha excreted by monocyte did not differ significantly from the control group (P > 0.05). CONCLUSIONS: After treatment by Ca(OH)2 for a week, the original immunological properties of LPS decreased significantly. High pH value of Ca(OH)2 played a critical role during the alteration of immunological properties of LPS.